Mimicking mycobacterial-infected neutrophil necrosis that occurs in active TB patients. (a) Human neutrophils isolated from peripheral blood were infected with M. bovis BCG and either left untreated or treated with H2O2 to induce necrotic cell death. Necrotic cell death was assessed by measuring the activity of the cytoplasmic enzyme LDH in culture supernatants (n = 3 *p > 0.01**p > 0.001). (b) Viability assessed by CFU of mycobacteria recovered from M. bovis BCG-infected neutrophils that were either treated with H2O2 to induce necrotic cell death or left untreated and succumbed to default apoptotic cell death. CFU were determined upon exposure to ambient air for the indicated time periods (n = 3, **p > 0.001, ***p > 0.0001). (c) Morphological analysis of human neutrophils either left uninfected or infected with M. bovis BCG without or with H2O2 treatment by H&E staining and SEM (n = 3).