Figure 2 | Scientific Reports

Figure 2

From: Enhanced tenacity of mycobacterial aerosols from necrotic neutrophils

Figure 2

Properties of mycobacterium-containing aerosols: (a) CFU of mycobacteria collected from the different levels of the Andersen impinger upon aerosolization in PBS at a 28 l/min airflow (n = 5; p > 0.001). (b) Percentages of live vs. dead mycobacteria as determined by live/dead staining upon aerosolization in PBS assessed immediately after settling for 10 min at the different impinger levels in comparison to control samples directly transferred from culture into PBS for viability staining before nebulization (n = 3, out of 100 mycobacteria, p > 0.0015). (c) Frequency of different mycobacterial cell numbers within aerosol particles, as depicted by electron microscopy analysis counted on levels 1–8 (n = 3, p > 0.0001). (d) Representative 3D reconstruction of airborne mycobacteria collected at different levels and analysis by AFM (the mean and standard deviation of three independent experiments are shown).

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