Analysis of engraftment and repopulation post MEISi expanded HSC transplantation. Murine LSK cells expressing CD45.2 allele were treated with effective dose of MEIS inhibitors and expanded for 7 days in HSC medium. These expanded HSCs were injected to NOD/SCID mice expressing CD45.1allele in a retro-orbital way. After 1 and 4 months, peripheral blood analysis was performed. Flow cytometry analysis was done with anti-CD45.2-FITCH and anti-CD45.1-PE. (A) Engraftment analysis. Engraftment and repopulation of HSCs were quantified based on the percentage of CD45.2+CD45.1− cells in the peripheral blood. (B) Repopulation of blood lineages. Quantification of B cells, granulocytes/macrophages and T cells derived from CD45.2+ HSCs in the recipient were determined. n = 4.