Figure 5 | Scientific Reports

Figure 5

From: Copper(II)-binding equilibria in human blood

Figure 5

Separation of human serum proteins by SEC. 4 x diluted human serum (a), 10 μM Cu•CP (b), 10 μM HSA plus 10 μM Cu(II) (c), 2.5 μM α2M tetramer plus 10 μM Cu(II) (d), 10 μM HSA, 2.5 μM α2M tetramer plus 10 μM Cu(II) (e). Conditions: incubation buffer 50 mM HEPES, 50 mM NaCl, pH 7.4; LC-ICP MS: column - 10 × 300 mm Superdex 200; elution buffer 200 mM NH4NO3, pH 7.4; flow rate 0.4 ml/min; injection volume 10 μl; UV at 280 nm (left axis and black lines) and Cu-63 (right axis and red line), monitored by ICP MS. Figure was created by program Origin 9 Pro (https://www.originlab.com/).

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