Abstract
Waste biomass from γ-polyglutamic acid production was used as an adsorbent to remove Cr(VI) from wastewater. Waste biomass was entrapped in sodium alginate to enhance performance. Orthogonal array design was used to optimize biosorption of Cr(VI) by immobilized waste biomass. The optimal adsorption conditions for immobilized waste biomass were as follows: pH 7.0, initial Cr(VI) concentration of 200 mg/L, 35 °C, waste biomass of 2 g/L, 60 min. Under these conditions, the absorption efficiency of Cr(VI) was 96.38 ± 0.45%. When the waste biomass was treated with 1 mol/L HCl for 1 h, the desorption rate could reach 94.42 ± 0.87%. It was shown that the adsorption kinetics followed the Freundlich adsorption model, indicating that the adsorption of Cr(VI) by bacteria was mainly based on multi-molecular layer adsorption. The absorption conditions of waste biomass were mild (pH 6.0–7.5, 20–35 °C) and easily operated. These investigations lay a foundation for reducing the pollution of γ-polyglutamic acid production, turning the biomass waste into a useful adsorbent for wastewater treatment.
Similar content being viewed by others
Introduction
Chromium (Cr) is a prominent metal pollution in modern industrialized world as a result of the extensive application of this metal in a variety of industries including metallurgy, mining, electroplating, printing and dyeing industries1. Wastewater discharged from these industries contain a significant amount of the hexavalent ionic form of this metal, which has attracted wide attention due to its toxicity, high carcinogenicity and mutagenicity2. Therefore, the effective treatment of wastewater containing Cr(VI) has become one of the hot issues in environmental sciences nowadays. Today, the most common methods for treating wastewater containing heavy metal ions are chemical precipitation, membrane filtration, liquid phase extraction, biosorption and ion exchange3. Among them, adsorption is the most promising method in the treatment of heavy metals, which already showed potentials in a many practices4. In particular, biosorption has attracted wide attention because of its low cost, easy operation and high removal rate5.
Gamma-polyglutamic acid (γ-PGA) is a water-soluble macromolecule material synthesized by microbial fermentation. It is synthesized by the polymerization of L-glutamic acid and D-glutamic acid via gamma-amide bonds6. Because of its reduced pollution to the environment, excellent biodegradability, film forming property, fibrogenicity, water-holding capacity and other special physical and chemical properties, it has now been widely used in medicine, agriculture, environmental protection, cosmetics, food and other industries7,8,9,10. With the increase of the demand and production of γ-PGA each year, a large number of waste biomass is produced, which unfortunately is another source of pollution if discharge directly without proper processing11.
Traditional methods for the treatment of Cr(VI) containing wastewater are expensive. In contrast, waste biomass is a byproduct of γ-PGA industry, and can drastically reduce treatment costs if it finds applications in the treatment of heavy metal-containing wastewater. Bacillus subtilis is the main producing strain of γ-PGA. Previous studies have reported the use of B. subtilis as an adsorbent. For example, Sukumar et al.12 reported the Cr(VI) removal using B. subtilis SS-1 isolated from soil samples of the electroplating industry. The removal efficiency was found to be 98.7% at 100 mg/L initial Cr(VI) concentration, pH 2 and 0.1 g/L biosorbent. Sivaprakash et al.13 reported that the B. subtilis biomass has the maximum biosorption rate of 48.64% at 100 mg/L initial chromium concentration, pH 2, 30 °C and 2 g/L biomass. The above-mentioned literature showed that B. subtilis had a strong adsorption capacity for Cr(VI). Therefore, the B. subtilis-containing waste biomass from γ-polyglutamic acid production has the potential to absorb Cr(VI) from wastewater, which was investigated in this study. The use of waste biomass to remove Cr(VI) from wastewater conforms with the concept of “treating wastewater with waste”. This concept has been applied by many scientists for the removal of Cr(VI). For example, Rossi et al.14 reported the removal of Cr(VI) using chemical and thermal treated discarded Saccharomyces cerevisiae at an efficiency of 99.66%. Mona et al.15 reported the removal of Cr(VI) by spent cyanobacterial biomass from a hydrogen fermenter at an efficiency of 80–90%. However, there is no report on the removal of Cr(VI) by waste biomass from γ-PGA production. The use of immobilized waste biomass in this work has an additional advantage in comparison with previous work: microbial cells are small, and are difficult to separate from aqueous solution, causing secondary pollution; while immobilizing cells can solve this problem and further lead to higher efficiency, stronger stability and better solid-liquid separation effect.
In this study, the adsorption of Cr(VI) in wastewater was performed using immobilized waste γ-PGA biomass as adsorbent. The adsorption conditions of waste γ-PGA biomass were optimized by orthogonal array design. The desorption conditions, adsorption isotherm and adsorption kinetics were studied in order to provide reference for the practical treatment of Cr(VI) in wastewater.
Results
Effect of time on adsorption rate
The process diagram of the whole experiment is shown in Fig. 1.The effect of adsorption time on the adsorption rate was investigated in reactions with the following parameters: temperature 25 °C, initial Cr(VI) concentration 50 mg/L, 3 g/L biomass pellets, pH 5, shaking speed 150 rpm. The adsorption results are shown in Fig. 2(a). It was shown that the adsorption rate of Cr(VI) to the immobilized biomass increased rapidly with time in the first 20 min. The adsorption rate reached 90.01 ± 1.13% at 60 min, and then increases slowly with time, reaching equilibrium at 180 min, when the adsorption rate reaches 92.06 ± 0.97%. Therefore, the adsorption time was set at 60 min in further optimization of Cr(IV) adsorption experiments.
Effect of temperature on adsorption rate
The effect of temperature on the adsorption rate was investigated. The results are shown in Fig. 2(b). It can be seen that the adsorption rate maintained at a high level at 30–40 °C, and reached maximum of 91.01 ± 0.44% at 30 °C. When the temperature exceeded 30 °C, the adsorption rate decreased with the increase of temperature.
Effect of pH on adsorption rate
From Fig. 2(c), it can be seen that the adsorption rate of waste biomass for Cr(VI) increases significantly with the increase of pH value when the pH value is 2–7. The maximum adsorption rate of 94.40 ± 0.83% was reached at pH 7. It is suspected that when the pH is low, the functional groups on the cell wall are protonated, while when the pH increased, H+ would dissociated from the functional groups, exposing more negative charged groups, which was beneficial to the binding of metal ions. However, too high pH would cause metal ions to form hydroxide precipitation, sabotaging the adsorption process16. Therefore, the optimum pH for the adsorption of Cr(VI) by waste biomass was 7.
Effect of waste biomass concentration on adsorption rate
Figure 2(d) shows that the biomass content is positively correlated with the removal rate of Cr(VI), but negatively correlated with the adsorption capacity. The reason might be that when the amount of metal ions in the solution was fixed, the smaller the concentration of the adsorbent was, higher number metal ions were surrounded around it, higher concentrations of the adsorbent could combine with metal ions to an larger extent, leading, to greater adsorption amount of the adsorbent per unit mass17. With these considerations, the dosage of bacteria was set at 2 g/L.
Effect of initial Cr(VI) concentration on adsorption rate
Figure 2(e) shows that with the increase of initial concentrations of Cr(VI) in solution, the adsorption rate of Cr(VI) gradually decreases, while the adsorption capacity first increases and then decreases. This might be because the number of active sites adsorbed by immobilized biomass was limited. When the initial concentration of Cr(VI) in solution was 100 mg/L, the number of active sites adsorbed by immobilized biomass was excessive in relative to Cr(VI). At this time, the adsorption rate of Cr(VI) was the highest (95.3 ± 0.72%) and the adsorption capacity was low (50.4 ± 0.32 mg/g). With the increase of initial concentration of Cr(VI) in solution, the adsorption sites on the surface of biomass become relatively smaller. Therefore, the adsorption rate decreases and the adsorption capacity increases. When the number of Cr(VI) in solution is equal to the adsorption sites on the surface of biomass, the adsorption capacity reaches saturation, and then the maximum adsorption capacity is 99.5 ± 0.52 mg/g. Thereafter, the adsorption capacity decreases with the increase of initial concentration of Cr(VI) in solution.
Adsorption optimization by orthogonal experiment
An orthogonal experiment was performed to optimize the combination of various parameters. Five factors at four levels, time (40, 50, 60, 70 min), temperature (20, 25, 30, 35 °C), pH (6, 6.5, 7, 7.5), biomass concentration (1.5, 2, 2.5, 3%) and initial concentration of Cr(VI) (200, 250, 300, 350 mg/L), were selected for optimization (Table 1).
Adsorption results indicated that the influential order of the five factors on the adsorption rate was A > D > B > E > C (Table 1). According to variance analysis, the time (p < 0.05) for the adsorption rate was significant, whereas B, C, D and E were not significant factors (Table 2). It can be see from Table 1 that k3 is time, k4 is the largest in temperature, k3 is the largest in pH, k2 is the largest in biomass concentration, and k1 is the largest in initial concentration of Cr(VI). Therefore, the optimal adsorption condition was deduced as: biomass 2 g/L, 60 min, 35 °C, initial Cr(VI) concentration of 200 mg/L and pH 7.0. In order to verify the optimal condition, adsorption under this condition was studied, and the adsorption rate reached 96.38 ± 0.45%. Therefore, this deduced condition was proved to be the best combination of different parameters.
Adsorption kinetics analysis
Adsorption kinetics is mainly used to analyze the relationship between adsorption rate and adsorption time. Adsorption rate controls the time needed to reach adsorption equilibrium. It is an important parameter to measure adsorption. In the study of adsorption kinetics, Lagergren quasi-first-order kinetic model and Lagergren quasi-second-order kinetics model are usually used to describe the relationship between the mass concentration of metal ions and time. The quasi-first-order kinetic model is based on the assumption that diffusion is controlled by adsorption. The quasi-second-order kinetics model is based on the assumption that the adsorption rate is controlled by chemical adsorption process. The linear forms of quasi-first-order kinetic model and second-order kinetics model are as follows18:
where k1 was pseudo-first order rate constant (h−1), k2 was quasi-second-order rate constant (g·mg−1·h−1), t was adsorption time (h), qt was adsorption amount at t time (mg·g−1), qe was adsorption amount at equilibrium (mg·g−1).
According to the parameters of Table 3, the fitting effect of quasi-second-order kinetic equation was better and R2 is higher, which indicated that quasi-second-order kinetic model could describe the adsorption process of waste biomass to Cr(VI) more accurately. According to the mechanism of quasi-second-order kinetic equation, it could be inferred that physical diffusion and chemical adsorption coexist in the process of adsorption of Cr(VI) by waste biomass. Chemical adsorption was dominant, and the effect of physical diffusion on the rate of chemical adsorption could be neglected.
Adsorption isotherm
Under the optimal adsorption conditions, the adsorption isotherm of chromium on bacteria was studied. The isothermal adsorption model can be used to describe the affinity between adsorbate and adsorbent and the surface adsorption characteristics19.
Langmuir adsorption model:
Freundlich adsorption model:
where qe was the equilibrium adsorption capacity (mg/g), ce was the equilibrium concentration of Cr(VI) in solution (mg/L), a and b were Langmuir adsorption constants, K and n were Freundlich adsorption constants.
Langmuir and Freundlich isothermal adsorption models are used to fit the experimental data20. The adsorption parameters are shown in Table 4. The R2 fitted by Freundlich isothermal model is 0.990. These results suggest that the adsorption of Cr(VI) by bacteria accorded with Freundlich adsorption model in a certain concentration range, indicating that the adsorption of Cr(VI) by bacteria was mainly based on multi-molecular layer adsorption.
Desorption characteristics of immobilized waste biomass
Results (Table 5) showed that deionized water, alkali solution and salt solution had poor desorption effect, while acid solution had better desorption effect, in particular 1 mol/L hydrochloric acid. The desorption rate was 94.42 ± 0.87% under this condition. This might be due to the fact that H+ in acid solution can compete with metal ions for binding sites on the surface of biomass cells, resulting in metal ions desorption. By using desorbents, the immobilized biomass can be recycled, improving efficiency. It is further shown that this method was non-destructive and could be used for multiple adsorption-desorption cycles.
Discussion
With the increase of γ-PGA demand, the production of γ-PGA increased year by year11. In the production of γ-PGA, a large number of waste biomass is produced. If it is discharged directly, the environment will be polluted severely. Traditional treatment method of waste biomass is generally used as fertilizer. However, the added value of the treated biomass as fertilizer is relatively low, and there are many similar products and the competition is fierce2. By developing it as a biological adsorbent, the added value can be greatly increased.
The comparison of different biosorbents that remove Cr(VI) was shown in Table 6. The order of adsorption rate of different biosorbents was Rossi et al. > Sukumar et al. > this study > Mona et al. > Sivaprakash et al. Although Rossi et al. reported a high adsorption rate of adsorbents, the following problems still exist: a long adsorption time (180 min), and the adsorbent could not be reused. There were similar problems existing with adsorbent reported by Sukumar et al. Regarding the optimum temperature and pH for adsorption, this study differed greatly from that of B. subtilis in the literature. The specific reasons might be as follows: (1) different strains of B. subtilis; (2) B. subtilis used in this study was a strain producing PGA, PGA was a heavy metal absorbent or chelating agent, and residual PGA in waste biomass might change the adsorption characteristics of bacteria. The adsorbent in this study outcompete the other adsorbents in the following ways: (1) The absorbent here has high adsorption rate. Although there was a certain gap between the adsorption rate (99.96% or 98.7%) reported in the literature (Table 6), the gap was not obvious. (2) The absorbent here has a low cost because it can offset the disposal costs of waste biomass from γ-PGA production. (3) The adsorbents in this study could be reused. In the experiment, the adsorbent was reused for 8 times continuously, and it still had good adsorption capacity (data not shown). (4) Short adsorption time (60 min). From Table 6, it could be seen that the adsorption time reported in other literatures was mostly 140–180 min, and the adsorption time in this study was only 60 min, greatly improving the adsorption efficiency. (5) Mild adsorption condition (pH 6.0–7.5, 20–35 °C). From the results of orthogonal experiments, pH and temperature were not significant factors, indicating that within the range of values, the changes of temperature and pH had little effect on the adsorption rate. It was also shown that the waste biomass had better adsorption rate in a wide range of pH (6–7.5) and temperature (20–35 °C). (6) Great economic potential. With the increase of γ-PGA production, a large number of waste biomass is produced. This provides a rich source of raw materials for adsorbent production. The adsorption process reported in this work is simple, has high adsorption efficiency, no secondary pollution, and has good operability. The adsorbent can be further reused, significantly reducing the cos. Therefore, the waste biomass from polyglutamic acid production shown here is an excellent adsorbent with great potentials for application.
In conclusion, the large amount of waste biomass from the polyglutamic acid industry was used as an adsorbent to adsorb Cr(VI) by trapping it in sodium alginate in an immobilization approach. Orthogonal array design was used to optimize the parameters on biosorption processes of Cr(VI) removal by immobilized waste biomass. The optimal adsorption conditions for immobilized waste biomass were as follows: pH 7.0, initial Cr(VI) concentration of 200 mg/L, 35 °C, biomass of 2 g/L, 60 min. Under these conditions, the absorption efficiency of Cr(VI) was 96.38 ± 0.45%. When the waste biomass was treated with 1 mol/L HCl for 1 h, the desorption rate could reach 94.42 ± 0.87%. It showed that the adsorption accorded with Freundlich adsorption model, indicating that the adsorption of Cr(VI) by bacteria was mainly based on multi-molecular layer adsorption. Physical diffusion and chemical adsorption coexist in the process of adsorption of Cr(VI) by waste biomass, chemical adsorption was dominant. The absorption conditions of waste biomass were mild (pH 6.0–7.5, 20–35 °C) and easily operated. The above results showed that the waste biomass from γ-PGA production was an excellent adsorbent. These investigations would lay a foundation for reducing the pollution of γ-PGA production, exploring a late-model for adsorbent production.
Materials and Methods
Preparation of biomass
Waste γ-PGA biomass was collected from Bioengineering Experiment Center of Shandong Jianzhu University, JiNan, China.
Waste biomass was washed twice with distilled water. After centrifugation for 10 min at 4000r/min, the cells were collected, washed twice with l% HCl, washed twice with distilled water, dried at 60 °C, ground into powder after cooling, and then dried for storage.
Preparation of immobilized bacteria pellets
Sodium alginate(3 g) was put into a flask containing 100 g distilled water and heated in a water bath at 80 °C for about 2 h to form a homogeneous and transparent solution.
The solution was cooled to 40 °C and mixed with 2 g waste biomass powder. The solution was absorbed by syringe and dripped into saturated boric acid solution containing 4% calcium chloride in ice-water bath. The solution was solidified for 24 h, then washed and stored for later use21,22.
Adsorption experiment
Different concentrations of Cr(VI) solutions (50–400 mg/L) were prepared, and pH values of solutions were adjusted to 2–10. Cr(VI) solution (20 mL) was absorbed and transferred into 100 mL flask. Then 3 g waste biomass pellets were put into the flask. In a water-bath oscillator at 10–50 °C and 150 rpm, Cr(VI) was absorbed by waste biomass pellets for 20–200 min. After reaching the reaction time, the sample was taken out and filtered. The residual metal ion concentration in the filtrate was measured by ultraviolet-visible spectrophotometer. The experiments were repeated three times, and the adsorption rate of Cr(VI) by waste biomass was calculated by the concentration of ions in the solution before and after adsorption:
where R was the adsorption rate (%), ρ0 was the mass concentration of Cr(VI) in solution before adsorption (mg/L), ρ1 was the mass concentration of Cr (VI) in solution after adsorption (mg/L).
Adsorption capacity:
where Q was the adsorption capacity (mg/g), ρ0 was the mass concentration of Cr(VI) in solution before adsorption (mg/L), ρ1 was the mass concentration of Cr(VI) in solution after adsorption (mg/L), v was the volume of solution(L), m was the dry biomass (g).
Orthogonal array design
Adsorption was optimized by orthogonal design and L 16(45) orthogonal table was shown in Table 121. According to the previous single-factor optimization experiments, they were used as the basis for selecting the level of orthogonal design. The five independent factors for adsorption (time, temperature, pH, biomass concentration and initial Cr(VI) concentration) were studied at three different levels and sets of 17 experiments were carried out (Table 1). All experiments were carried out in triplicate. The statistical analysis of the model was represented as an analysis of variance (Table 2).
Desorption test
After the immobilized biomass adsorbed Cr(VI) in the solution under the optimum adsorption conditions, the yeast was filtered from the adsorption solution. The biomass were equally placed in different desorbents (10 mL), oscillated elution at room temperature for 2 h. The desorption rate was calculated as follows: desorption rate (%) = desorbed Cr(VI) mass/adsorbed Cr(VI) total mass (%).
Analysis method
The concentration of Cr(VI) was determined according to the method reported in literature12.
References
Zhang, C., Wu, D. J. & Qiu, X. L. Stimulatory effects of amino acids on gamma-polyglutamic acid production by Bacillus subtilis. Scientific Reports 8, https://doi.org/10.1038/s41598-018-36439-4 (2018).
Zhang, L. et al. Effects of poly-gamma-glutamic acid (gamma-PGA) on plant growth and its distribution in a controlled plant-soil system. Scientific Reports 7, https://doi.org/10.1038/s41598-017-06248-2 (2017).
Wang, L. L. et al. Conformations and molecular interactions of poly-gamma-glutamic acid as a soluble microbial product in aqueous solutions. Scientific Reports 7, https://doi.org/10.1038/s41598-017-13152-2 (2017).
Yang, S. T. et al. Investigation of radionuclide Ni(II) sequestration mechanisms on mordenite by batch and EXAFS spectroscopy study. Science China Chemistry 55, 632–642 (In Chinese) (2012).
Li, Z. Y., Guo, S. Y. & Li, L. Study on the process, thermodynamical isotherm and mechanism of Cr(III) uptake by Spirulina platensis. Journal of Food Engineering 75, 129–136, https://doi.org/10.1016/j.jfoodeng.2005.04.003 (2006).
Cai, D. B. et al. A novel approach to improve poly-gamma-glutamic acid production by NADPH Regeneration in Bacillus licheniformis WX-02. Scientific Reports 7, https://doi.org/10.1038/srep43404 (2017).
Sung, M. H. et al. Natural and edible biopolymer poly-γ-glutamic acid: synthesis, production, and applications. Chemical Record 5, 352–366 (2010).
Ratha, P. & Jhon, D.-Y. Factors increasing poly–glutamic acid content of cheongguk-jang fermented by Bacillus subtilis 168. Food Science and Biotechnology 28, 103–110, https://doi.org/10.1007/s10068-018-0424-z (2019).
Massaiu, I. et al. Integration of enzymatic data in Bacillus subtilis genome-scale metabolic model improves phenotype predictions and enables in silico design of poly–glutamic acid production strains. Microbial Cell Factories 18, https://doi.org/10.1186/s12934-018-1052-2 (2019).
Cui, H., Dai, Y. & Lin, L. Enhancing antibacterial efficacy of nisin in pork by poly–glutamic acid/poly-l-lysine nanoparticles encapsulation. Journal of Food Safety 38, https://doi.org/10.1111/jfs.12475 (2018).
Carvajal-Zarrabal, O. et al. Treatment of vinasse from tequila production using polyglutamic acid. Journal of Environmental Management 95, S66–S70, https://doi.org/10.1016/j.jenvman.2011.05.001 (2012).
Sukumar, C., Janaki, V., Kamala-Kannan, S. & Shanthi, K. Biosorption of chromium(VI) using Bacillus subtilis SS-1 isolated from soil samples of electroplating industry. Clean Technologies and Environmental Policy 16, 405–413, https://doi.org/10.1007/s10098-013-0636-0 (2014).
Sivaprakash, A., Aravindhan, R., Rao, J. R. & Nair, B. U. Kinetics and equilibrium studies on the biosorption of hexavalent chromium from aqueous solutions using Bacillus subtilis biomass. Applied Ecology & Environmental Research 7, 45–57 (2009).
Rossi, A. D. et al. Chromium (VI) biosorption by Saccharomyces cerevisiae subjected to chemical and thermal treatments. Environmental Science & Pollution Research, 1–8 (2018).
Mona, S., Kaushik, A. & Kaushik, C. P. Biosorption of chromium(VI) by spent cyanobacterial biomass from a hydrogen fermentor using Box-Behnken model. International Biodeterioration & Biodegradation 65, 656–663 (2011).
Wang, Z. Y., Zhang, J. & Ting-Ting, S. U. The biosorption of cadmium(II) by discarded brewers yeast. Journal of Biology (2007).
Yun, W. U., Zhang, Z. X., Zhou, J. Z., Xiao-Ming, S. U. & Gulinazi. Study on Biosorption of Ni~(2+) by the Immobilized Saccharomyces cerevisiae Waste Biomass. Journal of Xinjiang Agricultural University (In Chinese) (2009).
Xing, D. Adsorption Research of Copper Ion by Waste Tea from Aqueous Solution and its Kinetics of Adsorption. Cleaning World (2017).
Ong, S. T., Lee, C. K. & Zainal, Z. Removal of basic and reactive dyes using ethylenediamine modified rice hull. Bioresource Technology 98, 2792–2799 (2007).
Dong, X. J., Zhu, C. & Lin-Wei, Y. U. Biosorption of Cu~(2+) by Immobilized Cladosporium sp. Chinese Journal of Applied & Environmental Biology 11, 493–497 (2005).
Xie, D. D., Liu, Y. Y., Cheng lin, W. U., Chen, P. & Jin kun, F. U. Studies of Properties on the Immobilized Saccharomyces cerevisiae Waste Biomass Adsorbing Pt~(4+). Journal of Xiamen University 42, 800–804 (In Chinese) (2003).
Lee, M. T., Chen, W. C. & Chou, C. C. Medium improvement by orthogonal array designs for cholesterol oxidase production by Rhodococcus equi No. 23. Process Biochemistry 32, 697–703, https://doi.org/10.1016/s0032-9592(97)00031-9 (1997).
Acknowledgements
This research was financially supported by State Key Laboratory of Microbial Technology (M2012-14), Shandong University.
Author information
Authors and Affiliations
Contributions
C.Z. and D.J.W. conceived of the study. C.Z. designed experiments, analyzed data, and performed experiments with assistance from H.X.R., C.Z. and C.Q.Z. drafted the manuscript. All authors read and approved the final manuscript.
Corresponding author
Ethics declarations
Competing interests
The authors declare no competing interests.
Additional information
Publisher’s note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Rights and permissions
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
About this article
Cite this article
Zhang, C., Ren, HX., Zhong, CQ. et al. Biosorption of Cr(VI) by immobilized waste biomass from polyglutamic acid production. Sci Rep 10, 3705 (2020). https://doi.org/10.1038/s41598-020-60729-5
Received:
Accepted:
Published:
DOI: https://doi.org/10.1038/s41598-020-60729-5
This article is cited by
-
Mullein leaf as potential biosorbent for copper(II) ions removal from synthetic solutions: optimization, kinetic and isotherm
International Journal of Environmental Science and Technology (2023)
-
A study on the biosorption efficacy of Brevibacillus brevis US575 biomass isolated from tannery effluents
Biomass Conversion and Biorefinery (2023)
-
Membrane Biosorption: Recent Advances and Challenges
Current Pollution Reports (2020)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.