Ablation of soft tissue tumours by long needle variable electrode-geometry electrochemotherapy: final report from a single-arm, single-centre phase-2 study

Standard electrochemotherapy (ECT) is effective in many tumour types but is confined to the treatment of small superficial lesions. Variable electrode-geometry ECT (VEG-ECT) may overcome these limitations by using long freely-placeable electrodes. Patients with bulky or deep-seated soft-tissue malignancies not amenable to resection participated in a single-arm phase-2 study (ISRCTN.11667954) and received a single course of VEG-ECT with intravenous bleomycin (15,000 IU/m2) and concomitant electric pulses applied through an adjustable electrode array. The primary outcome was radiologic complete response rate (CRR) per RECIST; secondary endpoints included feasibility, metabolic response, toxicity (CTCAE), local progression-free survival (LPFS) and patient perception (EQ-5D). During 2009–2014, we enrolled 30 patients with trunk/limb sarcomas, melanoma, Merkel-cell carcinoma, and colorectal/lung cancer. Median tumour size was 4.7 cm. Electrode probes were placed under US/TC guidance (28 and 2 patients, respectively). Median procedure duration was 80 minutes. Tumour coverage rate was 97% (29 of 30 patients). Perioperative side-effects were negligible; one patient experienced grade-3 ulceration and infection. One-month 18F-FDG-SUV decreased by 86%; CRR was 63% (95% CI 44–79%). Local control was durable in 24 of 30 patients (two-year LPFS, 62%). Patients reported an improvement in “usual activities”, “anxiety/depression”, and “overall health” scores. VEG-ECT demonstrated encouraging antitumour activity in soft-tissue malignancies; a single course of treatment produced high and durable responses, with low complications.


Patients and Methods
Patient selection. We prospectively enrolled the patients with measurable locally advanced or metastatic soft tissue tumours of any histotype who were not amenable to resection or unresponsive to conventional treatments according to the local multidisciplinary board. The size of the target lesion had to be comprised between 3 and 7 cm, while maximum tumour depth should not exceed 20 cm, with no infiltration across fascial planes. Eligible patients were adults with an Eastern Cooperative Oncology Group (ECOG) performance status of 0-2 and predicted life expectancy > 3 months, as well as adequate lung and renal function tests. The patients were excluded if any of the following conditions were present: a history of epilepsy, rapidly progressing visceral metastases, active infection, previous bleomycin chemotherapy up to the maximum cumulative dosage (400,000 IU), other local therapies within eight weeks; any systemic treatment four weeks before/after ECT. The protocol was approved by the local Ethics Committee (Comitato Etico Azienda Ospedaliera di Padova, No. 1886P; Supplemental Methods S1), and the study was conducted following the Good Clinical Practice Guidelines and Helsinki declaration (study identifier, ISRCTN.11667954). Treatment indication was agreed within the dedicated multidisciplinary team, and all patients provided signed informed consent.
Preoperative assessment and treatment planning. Patients underwent baseline evaluation through CT/MR and 18 F-PET-CT scan. ECT treatment plan relied on preoperative radiologic imaging, which included the following parameters: the most feasible approach for electrode insertion, the number of electrode probes (minimum two, maximum six, according to the characteristics of the pulse generator), their geometric configuration, and the distance between probes of each electrode pair. We aimed at reducing the total number of probes while ensuring optimal tumour coverage. When dealing with tumours indicatively larger than 27 cm 3 , the electrodes were retracted and reactivated along the same track to encompass the whole target volume, and additional placements were planned to extend the ablation volume further when required. Whenever possible, a 0.5-1 cm treatment safety margin was included to eradicate microscopic infiltration. The target lesion was assessed preoperatively by a radiologist to confirm the reliability of ultrasound (US) scan as a means to guide electrode placement in the operating theatre (Fig. 1); conversely, when US-based electrode tracking was deemed unsatisfactory, the procedure was scheduled in the interventional radiology suite, and the insertion of probes was performed under TC guidance.
Procedure. The procedure was carried out under tailored anesthesiological regimens, depending on tumour features and patient preference. The administration of bleomycin was in keeping with the European Standard Operating Procedure of ECT (ESOPE), with a slow intravenous infusion of 15,000 IU/m 2 (8). After eight minutes, the electric pulses were delivered using 20 cm-long needle electrodes with a 1.2-mm diameter, connected to a CE certified pulse generator (Cliniporator VITAE, IGEA, Carpi, Italy). Each electrode was inserted into or around the target lesion under US or TC guidance with the support of an on-site radiologist (Fig. 2). Based on previously published experience, we initially approached tumours with an array of five or six electrode probes to obtain optimal tumour coverage and avoid recurrences (15). The treatment was preceded by a train of low-voltage pulses (test phase) to verify the actual flow of the electric current between each electrode pair and the failing probes were repositioned until achieving an adequate current flow. During treatment, the voltage amplitude between each couple of electrodes could range from 500 V to 3,000 V depending on their distance. In each application, electric www.nature.com/scientificreports www.nature.com/scientificreports/ pulses were delivered over some tens of seconds. When required, the electrode array was juxtaposed multiple times according to pre-treatment plan to achieve tumour coverage (Fig. 3). Throughout the study and every three procedures, procedural details and patient outcomes were reviewed in an audit meeting.
Outcome evaluation. The feasibility of the procedure was graded as treatment delivery success rate (i.e. the number of patients in whom the target tumour was covered with electric fields exceeding the threshold for reversible electroporation). This assessment implied both an intra-operative evaluation of the electric current produced between electrode pairs ( Supplementary Fig. S1) and post-operative evaluation of the distribution of the electric field intensity around the tumour ( Supplementary Fig. S2). Tumour response assessment comprised a metabolic evaluation with 18 F-PET-CT performed between postoperative day 28-35, and radiologic evaluation using CT/MR (performed at 30 and 60 days following the Response Evaluation Criteria in Solid Tumors [RECIST] v1.1). Subsequent radiologic evaluations were agreed with the referring medical oncologist every three/four/ six months depending on disease course, patient status and eventual ongoing treatments. Patients were followed up at one week, 1, 2, 3 and 6 months, and every three months thereafter; those with ulcerated tumours were followed up by a nursing team for wound dressing. Toxicity was scored according to the Common Toxicity Criteria for Adverse Events (CTCAE v4.03). Patient-reported outcomes were collected at baseline, one month and two months utilising the EuroQoL quality of life scale (EQ-5D-3L) of the Euro Quality of Life Group (https://euroqol. org/eq-5d-instruments/eq-5d-3l-about/). Bleomycin pharmacokinetics. Blood and tumour samples were collected to evaluate bleomycin concentration through high-performance liquid chromatography (HPLC). Two 10 ml venous blood samples were taken at 8 minutes and 28 minutes after bleomycin infusion and centrifuged at 3000 rpm for 10 minutes; collected plasma was stored frozen at −20 °C. A tru-cut biopsy of the tumour was performed 8 minutes after chemotherapy infusion. The specimens will be analysed using a recently introduced and more reliable technique developed by another research group 17 , and results will be presented separately.
Study design. This single-arm phase 2 study was conducted using an exact single-stage design, according to Fleming 18 . The primary endpoint was antitumor activity in terms of CR rate. With a type I error of 0.05 and a power of 0.80, we set the maximum accrual at 30 patients, the largest response probability at 0.65 and the smallest response probability at 0.40. The acceptance threshold for efficacy was CR achievement in 17 or more patients.

Statistical analysis.
Continuous data were compared using the Mann-Whitney test or the Kruskal-Wallis test; Δ-SUV values were evaluated with the Wilcoxon test and the correlations with the Spearman's rank correlation coefficient. Categorical data were compared using Fisher's exact test. ECOG and EQ-5D-3L scores were evaluated by one-way repeated measures ANOVA with rank transformation, and variation in pain levels with pairwise Fisher's test with Bonferroni adjustment for multiple comparisons. Local progression-free survival (LPFS) was defined as the time from treatment administration to in-field disease progression. Survival curves were estimated with the Kaplan-Meier method. The effects of the duration of the procedure and CR achievement on LPFS were investigated with Cox regression models. The proportional hazard hypothesis was verified using Schoenfeld residuals (with P = 0.68 for local response and P = 0.52 for procedure duration, where P > 0.05 confirms the assumption of proportionality). A P-value lower than 0.05 was considered statistically significant. Analyses were performed using R 3.5 (R Core Team 2018. R: A language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria. Available online at https://www.R-project.org/).

Series.
We enrolled 30 patients between September 2009 and October 2014. Most of them had malignant melanoma or STS (43% each) ( Table 1). Fifteen patients (50%) had visceral metastases, nine (30%) had also skin tumour involvement. Median tumour size was 4.7 cm (range, 3.0-7.0), with lower values in tumours located in the lower limbs (P = 0.002). Three patient were receiving systemic treatment at the time of the VEG-ECT: these included a melanoma patient under targeted therapy and two sarcoma patients who were receiving paclitaxel with a mixed response (i.e. confirmed stable disease [SD] or partial response [PR] on visceral metastases but symptomatic progression on soft tissue as per RECIST criteria).  (Fig. 1). The maximum distance between electrodes cannot exceed 3 cm due to safety reasons and the needle probes must be placed in a parallel fashion to ensure homogeneity to the electric field.

Scientific RepoRtS |
(2020) 10:2291 | https://doi.org/10.1038/s41598-020-59230-w www.nature.com/scientificreports www.nature.com/scientificreports/ Treatment. The procedure was completed in all cases. Treatment parameters are summarised in Table 2. The intent was palliative in all but one patient, who received neoadjuvant VEG-ECT, followed by curative surgery. The median duration of the procedure was 80 min (range, 40-130), with no significant difference between subcutaneous and subfascial tumours (80 min [range, 45-120] vs 70 min [range, 50-130], P = 0.95). We used a median of two placements of the electrode array (range, 2-4), and the number of placements correlated with the size of the treated tumour (Rho 0.54, P = 0.002). In 18 patients (60%), one or more electrode probes had to be repositioned due to suboptimal electric current ( Supplementary Fig. S1). The post-procedural review of treatment data stored in the software of the pulse generator indicated that tumour coverage with electric fields was not achieved in one of 30 patients ( Supplementary Fig. S2), thus leading to a treatment delivery success rate of 97%. Four patients with malignant melanoma (n = 3) or STS (n = 1) received concurrent standard ECT within the same procedure for synchronous skin metastases. Following the on-trial audit meetings, we implemented some technical and procedural details. First, we introduced an echogenic trocar tip to electrode design to facilitate its insertion and enable US tracking within tissues. Second, we introduced some dedicated devices to stabilise the electrode array and to avoid the displacement of electrode probes due to muscle contraction ( Supplementary Fig. S3). Finally, to prevent any delay resulting from electrode repositioning and maximise tumour exposure to chemotherapy within the therapeutic window of bleomycin (20-40 minutes), we postponed chemotherapy infusion at the end of the "test pulse" phase.

Safety and toxicity.
There were no serious adverse events, either intraoperatively nor during the hospital stay, which lasted a median of 2 days (range, 1-4) (Supplementary Table S1, and Figs. S4, S5). A melanoma patient with a subcutaneous tumour in the lumbar region experienced intraoperative grade-2 bradycardia and received treatment with atropine; her following progress was uneventful.
Post-treatment pain. Overall, post-treatment pain was fluctuating ( Supplementary Fig. S5). In particular, after ECT, nine patients (30%) experienced no pain, whereas 16 (53%) and 5 (17%) reported mild and moderate pain, respectively. Pain level declined during hospital stay (P = 0.02), increased after one month (P = 0.002), and finally improved at two months (P = 0.02). At six months, only 8/29 evaluable patients had mild/moderate pain compared to 16/30 at baseline (P = 0.04). One melanoma patient, who underwent treatment for a proximal arm metastasis, reported grade-2 sensory neuropathy, which lasted three months and required corticosteroids, gabapentin and vitamin B12. Higher pain scores in the CTCAE scale and the EQ-5D questionnaire were associated with the increasing number of electrode placements (P < 0.001), previous chemotherapy exposure (P = 0.005), and use of six vs five probes (P = 0.04); we observed no association between postoperative pain and the anesthesiological technique (Supplementary Table S2).

Discussion
The results of this first-in-field study demonstrate that VEG-ECT can overcome two well-known limitations of standard ECT -large tumour size and deep anatomical location -and may represent an active and safe locoregional therapy for patients with soft tissue tumours when other treatments are not practicable. Over the last few years, electroporation-based procedures have evolved considerably 8,14 . Among the currently available approaches (standard ECT, irreversible electroporation [IRE], calcium electroporation, VEG-ECT and gene electrotransfer [GET]), standard ECT has become the most popular technique in clinical practice thanks to the ease of application, efficacy and safety coupled with an increasing number of indications 8,14 . However, the standard equipment does not allow targeting bulky (i.e., >3 cm) and deep-seated (i.e., >3 cm from the skin plane) tumours.
Our findings provide new insights into a novel electroporation-based treatment, VEG-ECT, and support the results of two previous studies in patients with liver and bone metastases. Edhemovic et al. reported an 85% CR rate with no relevant side effects in 16 patients with colorectal liver metastases (median tumour size, 1.7 cm) who were treated by either VEG-(n = 13) or standard (n = 3) ECT during an open surgical procedure 13 . In 2016, Bianchi et al. reported on 29 subjects with painful bone metastases who underwent one or repeated (34% of patients) VEG-ECT sessions under fluoroscopy or CT guidance 16 . The authors observed disease stabilisation in most cases and a significant improvement of pain control in 84% (20/24) of patients.
In the present study, the response rate after a single course of treatment was 93%, which is in line with our previous research on standard ECT in superficial STS, in which we treated 34 patients with a median tumour size of 4 cm and achieved a 92% response rate (12). Most notably, the results from the present study indicate a nearly doubled CRR (63% vs 32%), which was obtained through a single treatment session -whereas half of standard ECT patients underwent retreatment -and low complications. Importantly, the high rate of resolved tumours, which is likely the effect of the improved tumour coverage with electrodes, may reduce the need for retreatments and the associated toxicity, as demonstrated by the lower occurrence of side effects compared to standard ECT, where one-third of patients faced grade-3 toxicity 12 . Figure 5. Swimmer plot graph of response duration. Each bar represents a patient, and its length is proportional to the duration of local tumour control. Twenty-three of 30 patients received further oncologic treatments following VG-ECT after a median of 7 months (range, . These included the following: cytotoxic chemotherapy, n = 17; immunotherapy, n = 4; radiotherapy, n = 3; targeted therapy, n = 2; standard ECT, n = 1 surgery, n = 1; ILP, n = 1). The patient represented by the first upper bar underwent surgical resection (♦) of the target lesion treated with VG-ECT. Legend: CR, complete response; durable responder, a patient with CR/PR/SD persisting for at least six months; PR, partial response; ILP. Isolated limb perfusion; SD, stable disease.

Scientific RepoRtS |
(2020) 10:2291 | https://doi.org/10.1038/s41598-020-59230-w www.nature.com/scientificreports www.nature.com/scientificreports/ Although the functional disability caused by soft tissue metastases is generally mild, still they can impair patient quality of life due to day-to-day experience, progressive growth and ulceration as observed in more than one-third of our patients (Table 1). Since these lesions are a hallmark of advanced disease 1 , any palliative intervention should cause as little morbidity as possible, and the indication to treatment should be judicious. Interestingly, patient-reported outcomes in our study confirm the tolerability of VEG-ECT and suggest a potential palliative benefit.
Moreover, due to the novelty of the technique, incremental improvement and amelioration of its therapeutic ratio can be expected. According to our data, the number of probes and the number of placements of the whole electrode array correlated with post-treatment pain and local toxicity (Supplementary Table S2, S3). This observation suggests that researchers should exploit technical advancements aimed at reducing the invasiveness of the procedure (e.g. by reducing the number of probes through image-guided treatment planning or by using deployable electrode probes) to pursue future clinical application and maximise patient benefit 14 .
Remarkably, we achieved a durable remission in 80% of our cases (Fig. 5), which is a positive result in such an advanced disease setting, although the contribution of subsequent oncologic treatments and the follow-up schedule could have influenced the patient outcome. Local tumour control was slightly lower compared to our previous experience with standard ECT (2-year LPFS, 62% vs 72.5%); however the two populations are not comparable; moreover, we believe that, in selected cases, retreatment could have been proposed safely -although out of the study protocol -to prolong tumour control. However, the feasibility and safety of retreatment with VEG-ECT needs confirmation 16 .
At present, the clinical experience with this technique is still limited. In their pioneering study, Miklavcic et al. treated a 2-cm soft tissue metastasis from melanoma in a lower limb using four probes and achieved a 50% pathological response 15 . By comparing pre-treatment planning with measurements taken during the procedure, they revealed that small inaccuracies in electrode placement were responsible for suboptimal (i.e. 94%) tumour coverage. This observation has important implications for clinical practice because large tumour size is a common feature of soft tissue malignancies and a critical aspect in the application of electroporation-based treatments. An analysis of patients with soft tissue metastases treated at the Ohio University Medical Center revealed that 32% (38/118) had lesions larger than 3 cm, while in the experience of the Royal Orthopaedic Hospital of Birmingham, 78% (78/100) of patients had tumours exceeding five centimetres 2,19 . Finally, we confirm here that tumour size remains significantly associated with response to ECT, also when using the VEG modality ( Table 3).
As expected from previous studies with ECT, the only toxic effects were local site reactions. Of note, however, bulky lesions may develop a considerable amount of tissue necrosis due to ECT sustained antitumour activity, with the inherent risk of ulceration, infection, and delayed healing (Supplementary Table S1). Although numbers are too small to draw firm conclusions, we found that local toxicity correlated with pre-existing tumour ulceration, sarcoma histotype, number of electrode placements as well as the duration of the procedure (Supplementary Table S3). The  Table 2. Treatment (n = 30 procedures). *The duration of the procedure was retrieved from electronic medical records and calculated as the interval between the end of the preparatory phase of anaesthesia (whatever the technique applied) and the end of the application of electric pulses. °The bolus of bleomycin (15,000 IU/m 2 ) was administered intravenously over one minute. † The number of electrodes correlated with tumour size (P = 0.002); in the five-electrode group, the median tumour size was 45 mm (range, , while in the sixelectrode group it was 55 mm (range, 40-70 mm). ‡ Number of planned applications of the whole electrode array to treat the target tumour. ⁋ Patients who needed unplanned reinsertion of one or more probes during the procedure due to suboptimal current flow in the test pulse phase or during treatment delivery.
Scientific RepoRtS | (2020) 10:2291 | https://doi.org/10.1038/s41598-020-59230-w www.nature.com/scientificreports www.nature.com/scientificreports/ higher toxicity recorded in sarcoma patients could be explained by the more frequent use of radiotherapy in this subgroup, coupled with the higher presence of baseline tumour ulceration. Overall, these observations could inform patient selection in future studies and help to improve the palliative benefit of VEG-ECT by excluding or informing the subjects with ulcerated or skin-infiltrating tumours, reducing bleomycin doses in those previously irradiated, or implementing treatment planning through the reduction of the number of electrode probes.
Nowadays, thanks to the continuous improvement of patient survival produced by systemic therapies, soft-tissue metastases are being encountered more frequently. They occur in 1.2-6.6% of STS patients 1,19 and, together with skin and lymph nodes, represent a primary site of diffusion of cutaneous melanoma [20][21][22][23] , and a possible modality of spread for carcinomas (e.g. lung, breast, kidney, and colon) and lymphoproliferative disorders 1,2,19,24 . The management of these lesions is challenging because there are few clear-cut solutions and a multitude of therapeutic options. Generally, surgical resection deserves primary consideration, particularly in patients with oligometastatic disease, but systemic treatment still represents the sole option in the majority of cases 3,21,25 . In patients with ulcerated or bleeding tumours, a short course of intense radiotherapy may confer palliative benefit 26 . Alternatively, hyperthermia, hypoxia, chemotherapy or radiation can be applied in synergistic approaches including hyperthermic isolated limb perfusion, isolated limb infusion, and regional hyperthermia plus radiotherapy or chemotherapy [27][28][29] . In the past, local immunotherapy with interleukin-2 has been used sporadically, achieving CRRs exceeding 60% in melanoma 30 . Finally, also ablative therapies lend themselves to the treatment of soft tissue metastases. These interventional radiology procedures include radiofrequency, microwave and cryoablation, as well as arterial embolisation and IRE 4,5,31,32 . These techniques greatly vary in complexity and fundamental principles, and should only be applied within a shared multidisciplinary algorithm 33 .  Table 3. Tumour response according to patient characteristics and treatment parameters. SD, stable disease; PR, partial response; CR, complete response; Tx, treatment; CT, chemotherapy; ILP, isolated limb perfusion. *Data expressed as number (%) or median (IQR). °Number of planned placements of the whole electrode array to cover the target volume. † Patients in whom it was necessary to replace one or more probes due to low electric current.
Scientific RepoRtS | (2020) 10:2291 | https://doi.org/10.1038/s41598-020-59230-w www.nature.com/scientificreports www.nature.com/scientificreports/ VEG-ECT may represent an attractive option for surgical oncologists and interventional radiologists, with some potential merits. First, tissue preservation. Being a non-thermal ablative modality, it relies on reversible electroporation and a selective cytotoxic effect on replicating cells. Its safety in bone and liver tissue is documented in animal models and human studies 13,16,34,35 . A second advantage includes the coverage of large target volumes. Indicatively, a 5-electrode array (four peripheral electrodes in a 3-cm square fashion with a centrally placed electrode) with a 4-cm active tip is capable of targeting a 36-cm 3 tissue volume. Third, the possibility to refine the ablation zone by selectively placing additional electrode pairs. Importantly, this enables VEG-ECT to achieve precise tumour coverage and include adequate safety margins while respecting surrounding tissues. Finally, repeatability and combination with standard ECT within the same procedure offer the unique opportunity to treat tumours of different size and depth within the same procedure. This combination is particularly advantageous in patients with melanoma or sarcomas who present with soft tissue metastases, which are heterogeneous in size and anatomical location and often are scattered.
At the same time, the limitations of this approach must be acknowledged. First, VEG-ECT entails the application of multiple electrodes, which could make the procedure cumbersome and time-consuming in some patients. This, nevertheless, soon could be overcome by the adoption of expandable catheters or more sophisticated tools for treatment planning 14 . Another concern relates to the insertion of relatively large probes and delivery of high voltages, which makes VEG-ECT more invasive and, conceivably, less tolerable compared to standard ECT. As a result, clinicians should adjust the anesthesiological management. In our experience, however, mild general or spinal anaesthesia without neuromuscular-blocking drugs ensured patient comfort and prevented anatomic motion during the procedure. Of note, the direct intense muscle stimulation produced by electric pulses may displace electrode probes and compromise treatment application. Adequate tumour coverage with electric fields requires not only accurate electrode insertion 12,36 but also their stability during the phase of pulse delivery, which generally lasts some tens of seconds. Finally, treatment planning, image-guidance, individual electrode placement, and coordination with the software of the pulse generator inevitably add some complexity to the procedure. Nevertheless, by determining the best approach and electrode configuration, these are fundamental aspects of VEG-ECT technique and result particularly helpful when dealing with tumours located in challenging anatomical sites.
Interestingly, different supporting tools and navigation systems have been proposed to add precision and safety to VEG-ECT. These advancements will be particularly helpful in the treatment of intra-abdominal tumours such as liver malignancies and pancreatic cancer, or some forms of head and neck cancer 14 . As to soft tissue malignancies, according to our experience, an accurate and safe electrode placement can be achieved through US guidance, at least in patients with suprafascial lesions, while CT assistance can be reserved for selected patients with intramuscular lesions.
Our results have the obvious limitations of a single-arm phase 2 design and include its small scale, patient heterogeneity, the absence of a control group and independent radiological review, the novelty of the procedure, which inevitably implied a learning phase as in other therapies 32 and on-study technique modifications. Admittedly, other variables might have influenced treatment outcome, including distribution of bleomycin and electric current within inhomogeneous tumour tissues 37 , and a discrepancy between pre-treatment plan and actual electrode placement. On this regard, a preliminary experience in patients with liver metastases suggests that US scan holds promise for real-time assessment of the electroporation process 38 , while experimental evidence in animal models indicates that magnetic resonance electrical impedance tomography (MREIT) may be a reliable instrument to predict electric field distribution 39 . Conversely, PET-CT scan performed at one month confirmed the strong antitumour effect of ECT but provided no additional predictive information regarding final treatment outcome; therefore, we believe that a single radiological evaluation may be appropriate to evaluate VEG-ECT response in routine clinical practice.
Finally, the availability of a highly active and safe locoregional therapy is particularly intriguing in the light of the proliferation of new targeted therapies and immunotherapies 14,40 , which have improved patient outcome and are opening new avenues for synergistic approaches aimed at consolidating local disease control and prolonging patient survival 6,7,41 .
Collectively, our data provide evidence that the novel ECT approach is feasible and safe in patients with locally advanced or metastatic tumours of the soft tissue. A single course of treatment led to complete resolution in 60% of cases, with low complications. These outcomes support VEG-ECT as a promising locoregional therapy for patients with bulky or deep-seated soft tissue metastases not amenable to surgical resection. Improvement of treatment planning and image guidance, real-time assessment of tissue electroporation, and further technical evolution have the potential to streamline the procedure. Additional detailed investigations are warranted to standardise the technique, define selection criteria and incorporate VEG-ECT into novel effective strategies. Our results highlight the benefit of VEG-ECT, advance the state-of-the-art in this field and lay the groundwork on which to plan future studies.

Data availability
The individual patient data set is available upon reasonable request from the corresponding author.