Predicting the effects of invasive ecosystem engineering species in new bioregions has proved elusive. In part this is because separating biological effects from purely physical mechanisms has been little studied and yet could help predict potentially damaging bioinvasions. Here we tested the effects of a large bio-engineering fanworm Sabella spallanzanii (Sabella) versus worm-like structures (mimics) on gas and nutrient fluxes in a marine soft bottom sediment. Experimental plots of sediment in Hauraki Gulf (New Zealand) were used to test the hypothesis that ecosystem engineers negatively influence benthic ecosystem function through autogenic mechanisms, facilitating activity by biofouling organisms and competitive exclusion of native infauna. Enhanced physical structure associated with Sabella and mimics increased nitrogen fluxes, community metabolism and reduced denitrification from 23 μmol m−2 h−1 to zero at densities greater than 25 m2. Sabella plots on average had greater respiration (29%), NH4 release (33%), and greater NO3 release (52%) compared to mimics, suggesting allogenic (biological) mechanisms occur, but play a secondary role to autogenic (physical) mechanisms. The dominance of autogenic mechanisms indicates that bio-engineers are likely to cause significant impacts when established, regardless of fundamental differences in recipient regions or identity of the introduced bio-engineer. In the case of Sabella spallanzanii, compromised denitrification has the potential to tip the balance of net solute and gas exchanges and cause further ecological degradation in an already eutrophic system.
Ecosystem engineers create, destroy or otherwise modify habitats, often exerting a strong positive control on the structure and functioning of entire ecosystems1,2 and are recognised as some of the most damaging groups of invasive species3,4. However, when ecosystem engineers invade new environments the spectrum of physical, physico-chemical and ecological consequences makes it difficult to interpret the net balance of changes and quantify or predict impacts4,5. Even when much is known about a single introduced bio-engineering species, the effects on recipient ecosystems that vary across latitudes, regions, and local environmental gradients6,7,8 may, themselves, be highly variable9,10. Despite this context-dependence, impacts observed in one location are regularly used to infer potential impacts of introduced species at other locations7,11. In fact, serial invaders (e.g., Styela clava, Sabella spallanzanii, Undaria pinnatifida, Sargassum muticum, Carcinus maenas, Caulerpa taxifolia) are often used as model species for generalising traits that result in significant impacts to native ecosystems and are frequently included in ‘unwanted-organism lists’12,13. Elucidating the dominant mechanisms of ecological impacts from bio-engineers may demonstrate the utility of global lists of unwanted marine organisms for prioritising management efforts.
The impacts of introduced species on the ecology of recipient communities are most commonly reported as changes in native species populations, e.g., declines or extirpations of native species following outbreaks of non-native predators or competitors3,9,14. This approach is problematic when attempting to translate introduced species impacts on a global scale, as each recipient ecosystem has highly variable species assemblages and ecosystem structures. This is particularly true when native ecosystem engineers are affected by invasions. Research on native communities shows that the presence and abundance of ecosystem engineers (rather than species richness per se) is the predominant driver of ecosystem function (e.g., earthworms1, legumes15, marine bioturbators16). In many examples, ecosystem engineers that enhance the heterogeneity of above or below surface structures, contribute disproportionally to ecosystem functions, and may buffer anthropogenic stressors17. Introduced species that disrupt native ecosystem engineers will, therefore, have greater consequences on the functioning of native ecosystems18. In this sense, identifying the alteration of ecosystem functions performed by native functional groups (e.g., bioturbators, habitat-formers) may enable generalisation of introduced species impacts over broad scales, allow more accurate predictions of potential impact, and improve prioritisation of limited resources for management of marine bioinvasions.
One of the key ecosystem services performed by marine soft-sediment systems is the processing and potential loss of nitrogen19. The sediment-water interface is a focal point for critical ecosystem functions in benthic marine systems16,20, with dissolved nutrients (e.g., ammonium, phosphate) moving across the surface21,22 and particulate material accumulating beneath it. The balance of the fluxes in and out of the sediment is heavily modified by the seafloor biota which physically bioturbate the sediment matrix, altering the thickness and surface area of redox boundaries, and movement of water (i.e., active transport, bioirrigation23). The magnitude of exchange processes can be disrupted by elevated inputs of dissolved and particulate materials (e.g., through anthropogenic eutrophication or sedimentation24), or through changes to the biotic structure of benthic communities23. Species that are functionally unique (physically or physiologically) can cause major shifts in the balance of particulate and dissolved material with far-reaching consequences to the provision of ecosystem services25.
There are several examples of alteration of ecosystem functions by benthic invaders, for example: reduced nutrient uptake capacity by an introduced seagrass26; reductions in water column chlorophyll a by a introduced mollusc27; and reduced denitrification efficiency by a introduced tubeworm28. Yet the mechanisms responsible for shifts in ecosystem functioning are often less clear than the magnitude of change. Introduced species are capable of affecting ecosystem functioning through several mechanisms; direct trophic impacts29, indirect trophic impacts30, competition31 or facilitation of native or introduced organisms32, autogenic engineering and allogenic engineering3. Here we focus on the dominant mechanisms of ecosystem engineers: allogenic and autogenic. Autogenic engineering refers to the physical alteration of the environment by the bodies of ecosystem engineers (e.g., creation of biogenic habitat33,34). Allogenic engineering is the alteration of physical environmental properties via mechanical (e.g., filtering of particulate matter35,36 or chemical means (e.g., deposition of faeces28). For example, autogenic engineering can be realized through reductions or loss of key native macroinvertebrates through competitive exclusion37, which has the potential to modify the sediment-water interface and reduce the rate of several critical functions. Equally, introduced ecosystem engineers can facilitate both native and introduced species with potential for both positive32 and negative consequences38. Species that modify and create habitats provide a resource (e.g., space), which in the context of marine soft-sediment systems, has the potential to exclusively or simultaneously affect above sediment and below sediment organisms and processes. Autogenic engineering can also be realized through modification of the physical environment, which include changes to hydrodynamics that can influence boundary layer thickness, orbital bed velocities39,40, sediment accumulation/erosion41, and changes in light penetration to the benthos35,36. Allogenic engineering includes chemical modification effects such as increased production of organic rich biodeposits or enhanced excretion of ammonium28; interception and consumption of organic material27; and alteration of pore water solute concentrations and gradients (via oxygen respiration, ammonium excretion).
Experimental manipulations of introduced species and structural equivalents (i.e., mimics) allow the disentanglement of physical and biological effects3, while the simultaneous measurement of flux rates and community composition shifts may uncover the relative influence of chemical alteration by introduced species and loss of key processes when native species are displaced. Experiments separating the structural complexity of introduced species from metabolic processes have revealed that physical engineering is an important contributor to the impacts of introduced species34,42. However, to our knowledge no study has manipulated densities (i.e., a gradient of densities) of real and mimic structure-forming introduced species in situ and measured both flux rates and community composition shifts. There are, however, studies which have examined the relative contribution of living bivalves and non-living shells to denitrification rates, which indicate that internal tissues and shell material are colonised by denitrifying bacteria43,44. We postulate that an experimental design manipulating introduced species density and incorporating live introduced species and structural equivalents (mimics) will enable an assessment of the relative impact pathways of introduced bio-engineering species on nitrogen cycling of benthic systems, with broad relevance for inferring impacts to recipient ecosystems.
The tube-forming polychaete worm Sabella spallanzanii (Gmelin, 1791, Polychaeta: Sabellidae) was first detected in Waitemata Harbour, Auckland, New Zealand in 200945 and has since spread rapidly across artificial, hard, and soft-sediment benthic habitats towards open coast environments. This species contrasts starkly to native communities in these areas which have been modified by fishing activities, sedimentation, and nutrient input, but also differ greatly from structure forming bivalve species that once occurred in high densities46. Current evidence suggests that Sabella greatly increases NH4 excretion and oxygen consumption, reduces denitrification efficiency, and is associated with shifts in infaunal composition28. These findings were interpreted as a response to the filtering capacity of Sabella and the biofouling supported by its tubes but separating the role of the living worm, biofouling attached to its tube and alterations to infauna is less straightforward. The rapid expansion observed in northern New Zealand has important implications for the greater Hauraki Gulf marine ecosystem surrounding Auckland (New Zealand) which has seen dramatic increases in nutrient additions from land-use change47. Threats to nutrient removal capacity at the sediment-water interface may exacerbate eutrophication and hypoxia issues in the region47. Disentangling the relative mechanisms of impact, including critical density thresholds, has important implications for managing the effects of organic nutrients on coastal ecosystems, particularly the setting of acceptable limits of nitrogen inputs from anthropogenic activities. Furthermore, identifying the dominant ecosystem engineering mechanisms will help determine the potential for broader inference of introduced species impacts on global scales, where impacts related to reductions in native engineers, or facilitation of native or introduced species have context specific impacts, but impacts related to physical and chemical alteration have general relevance to benthic ecosystems.
The three theoretical models of impact associated with introduced ecosystem engineers relate to two mechanisms of autogenic engineering (‘1’ Displacement or facilitation of biotic communities; ‘2’ Alteration of the physical environment) and one mechanism of allogenic engineering (‘3’ Chemical alteration). Each mechanism is expected to produce a unique combination of responses across live bio-engineers (Fig. 1a for models ‘1’, ‘2’, and ‘3’) and mimics (Fig. 1b for models ‘1’, ‘2’, and ‘3’) and would differentially impact flux rates and community composition if acting alone. For impact mechanism ‘1’, displacement or facilitation of biotic communities, it would be expected that the presence of live Sabella (Fig. 1, model ‘1’ a) and mimics (Fig. 1, model ‘1’ b) would affect community composition and subsequently flux rates. For impact mechanism ‘2’, alteration of hydrodynamic regimes, both Sabella (Fig. 1, model ‘2’ a) and mimics (Fig. 1, model ‘2’ b) would each impact flux rates through elevated accumulation of organic material39,40, but would have minimal consequences on biotic community composition. Impact mechanism ‘3’, chemical alteration, would affect flux rates in the live Sabella treatment only (Fig. 1, model ‘3’ a) and not mimics (Fig. 1, model ‘3’ b), but with no differences in community composition between treatments. Here we use an experimental density gradient of Sabella and mimics and examine shifts in flux rates and key fauna to test these three theoretical models of impact mechanisms. We hypothesize that the autogenic physical impact pathways will be the dominant mechanism of impact (model ‘1’ and ‘2’).
Nutrient cycling and community oxygen consumption rates were generally greater in the presence of live Sabella spallanzanii (‘Sabella’) compared to structural equivalents (‘Mimics’). Flux rates of oxygen, nitrogen, and phosphorus had a linear positive relationship with worm density, but denitrification rates declined with increasing densities of Sabella and mimics (Fig. 2). There were, however, some key differences in solute fluxes between Sabella and mimics. Community respiration (O2, Fig. 2a; Table 1) and NO3 flux rates were greater in Sabella plots (Fig. 2e; Table 1), while dissolved reactive phosphorus (DRP) flux rates were greater in plots with mimics (Fig. 2c). Sabella density had little effect on DRP but increased with density of mimics (Fig. 2c, Table 1). Ammonium (NH4, Fig. 2d, Table 1) and total DIN flux (Fig. 2f, Table 1) increased with density of both Sabella and mimics (Fig. 2b, Table 1), but the significant density × treatment interaction suggested that the increase in fluxes of Sabella plots was greater than mimics. Rates of denitrification declined with increasing density of Sabella and mimics and at densities greater than 20–25 worms per m2 denitrification rates approached zero for both Sabella and mimics (Fig. 2b).
Multiple linear regression results revealed that densities of Sabella and mimics, or interactions between treatment and density, were the primary drivers of changes to ecosystem functions (Table 1). Model selections revealed variation between flux variables, with O2 flux explained by density, and a significant treatment × density interaction. Sediment organic content also explained O2 flux, or co-varied with experimental treatments. N2 flux was explained by density, with the clay composition also explaining or co-varying with treatments. DRP flux was driven by density, and a treatment × density interaction, with phaeopigments also explaining or co-varying. NH4 flux was explained by density and a treatment × density interaction. Like NH4, NO3 was explained by density, and a significant treatment × density interaction. Total DIN showed similar explanatory variables as NH4, likely driven by the much higher flux rates of NH4 compared to NO3. Although there was no significant relationship between chlorophyll a and density, there was a significant treatment affect, with Sabella plots exhibiting elevated sediment chlorophyll a (Supplementary Fig. S1).
Unlike other flux variables, denitrification efficiency (the proportion of nitrogen fluxed into the water column as N2 compared with the total dissolved inorganic nitrogen [Ross et al.28]) was not affected linearly with increasing worm density and showed a fast decline with increases in Sabella and mimic densities (Fig. 3). Beyond approximately 10 worms per m2, denitrification efficiency fell below 5% for Sabella plots. Mimics had a broader range of denitrification efficiency with increasing density, but beyond densities of 20 per m2 denitrification efficiency was below 5%.
Combined flux profiles (i.e., the multivariate response of combined fluxes; NH4, NO3, N2, O2, DRP) showed high separation between treatments, with much greater separation between mimics and Sabella. The integrated flux profiles of several plots with high densities of living Sabella (establishment densities of 35–50 worms per m2) were noticeably separated from all other plots. Overall there was a significant difference in the flux profiles between treatments (Psuedo-F1,23 = 13.5, p(perm) = 0.001), and density (Psuedo-F1,23 = 2.6, p(perm) = 0.03). Flux rates of Sabella plots were explained by higher biomass, phaeopigments, chlorophyll a and smaller sediment fractions (Fig. 4, Supplementary Table S2). Variation in flux rates was also related to variations in species assemblages (Fig. 4). Flux rates of control plots were significantly associated with the presence of a burrowing crab (Pilumnus novaezealandiae), a burrowing holothurian (Taeniogyrus dendyi), and the brushworm Phylo novaezealandiae (Fig. 4). Conversely flux rates of plots containing Sabella and mimics were associated with biofouling organisms such as ascidians, hydroids and porifera (Fig. 4). Flux rates of mimic plots were also associated with the small polychaete family Myriochele and the burrowing heart urchin Echinocardium cordatum (Fig. 4).
Despite shifts in species identity across treatments, there were neutral trends in species richness and biodiversity metrics (Shannon diversity and Pielou’s evenness) with increasing densities (Supplementary Fig. S3). There was, however, a significant increase in overall biomass with increasing densities of Sabella, and mimics (Supplementary Fig. S3, Panel A). Although the accumulated biomass with increasing densities of Sabella included the live worms themselves, the strong trend of increasing biomass with density of mimics showed the large contribution of biofouling organisms (e.g., ascidians, sponges and hydroids attached to Sabella or mimics).
Six months after establishing experimental density gradients of non-native fan worms (Sabella spallanzanii) and structural analogues (mimics), several changes to seafloor sediment characteristics, bioturbating species and ecosystem functions were observed. Notably, effluxes of dissolved inorganic nitrogen (ammonium and nitrate) across the sediment-water interface, and rates of community respiration increased significantly with worm density. In contrast, rates of denitrification (a microbially mediated ecosystem function that eliminates bioavailable inorganic nitrogen from the system) declined. This was the first study of the effects of Sabella on ecosystem functioning in New Zealand, where it was first recorded 10 years prior. Our findings were consistent with effects predicted for invasive ecosystem engineers4 and similar to results of experiments on Sabella conducted in Port Phillip Bay, Australia by Ross et al.28. We observed much greater reductions in denitrification, and reduced flux of dissolved reactive phosphorus compared to Ross et al.28. However, much like the findings from Guy-Haim et al.4 there were neutral effects of an invasive ecosystem engineer on several biodiversity metrics, likely associated with simultaneous reductions in infaunal biodiversity and gains in biofouling biodiversity.
Invasive species with ecosystem engineering traits have been linked to high magnitude ecological impacts3,14,42. In our study, Sabella and structural equivalents (mimics) altered the composition of benthic infauna and epibiota and facilitated tube fouling organisms, which was strongly associated with changes to biogeochemical cycling. Our findings support the autogenic engineering hypotheses that changes in habitat availability for other organisms is a dominant component of structure forming introduced species impacts. There was evidence of reductions in large bioturbating species by Sabella and mimics and increasing biofouling biomass (impact mechanism ‘1’). Interestingly in this example the facilitation of largely native fouling organisms (exceptions were two introduced ascidians Symplegma brackenheilmi and Styela clava) was associated with negative consequences to important ecological functions (e.g., denitrification). However, the process of denitrification is driven by bacterial activity and it is unclear from this study how bacterial denitrification is being impacted by Sabella or mimics.
Rates of denitrification decreased linearly with increasing structures, both living (Sabella) and analogues (mimics), while denitrification efficiency declined exponentially. This contrasts with the positive linear increase in sediment denitrification with increasing bivalve biomass (clams, Ruditapes philippinarum) and no net change to denitrification efficiency48. Likewise, denitrification potential was either unchanged or increased in the presence of the burrowing bivalve Austrovenus stutchburyi49. Both of these examples include burrowing clams which have little above sediment structure, however, another bivalve Atrina zelandica which protrudes from sediments can also potentially contribute to enhanced denitrification50,51. Density gradients of above-sediment, structure-forming bivalves Atrina zelandica have also been shown to physically influence sediment dynamics through reductions in resuspension39, yet similar mechanisms of physical changes between bivalve molluscs and tube-forming polychaetes (i.e., Sabella) are having contrasting biogeochemical outcomes.
Disentangling cause and effect when examining changes in fluxes and biogeochemistry is difficult due to the complexity of linked interactions and opposing processes (e.g., photosynthesis and respiration). The addition of live Sabella likely affected solute fluxes in both direct (respiration, excretion) and indirect ways (altered hydrodynamics, sediment characteristics, and macro- and micro-organism communities), but the similarly large changes in benthic fluxes associated with mimics suggest that autogenic engineering mechanisms dominate. Species with characteristics of ecosystem engineers modify the physical environment through two mechanisms, autogenic and allogenic engineering1. Autogenic engineering is the alteration of physical aspects of the environment by the bodies of the engineers themselves (e.g., alteration of flow regimes by Sabella tubes or provision of habitat for other organisms), whereas allogenic engineering is the alteration of physical environmental properties via mechanical or chemical means (e.g., extraction of particulate material by Sabella)1. Shifts in bioturbating ecosystem engineers (e.g., Echinocardium cordatum16) suggest the autogenic influence of the introduced species (Sabella) may be partially mediated through shifts in the composition of native fauna, flora and microbiota52 which play important functional roles in nutrient cycling, particularly removal of bioavailable nitrogen (i.e., denitrification). Alterations of infaunal community composition was observed by Ross et al.28, and our results suggest this may be associated with a combination of crowding and interception of suspended particulate organic material by Sabella and biofouling on its tubes. The dominance of autogenic impact mechanisms may have broader implications for understanding the generality of impacts from bioinvasions and suggests that bio-engineering species which impact one region are highly likely to affect other bioregions.
Multivariate analysis of combined flux profiles (i.e., NO3, NH4, DRP, O2 and N2) showed that plots of living Sabella separated from plots of mimics indicating that the sum of solute changes was uniquely affected by Sabella. It is possible, that nuances of packaging and deposition of psuedofaeces53, or suitability of the tube or soft tissue for colonisation by nitrifying or denitrifying bacteria43,44 may be responsible for the separation of flux profiles between live Sabella and mimics. There is also evidence that Sabella produce a mucus with antibacterial properties, which could potentially upset the balance of nitrifying/denitrifying bacteria53, yet our study suggests that if this mechanism operates, it is secondary to the physical influence of tube structure. Combined, our results reveal that structure-forming introduced species represent a major threat to ecosystem functioning of marine ecosystems, as has been postulated by others3,4,14. Such traits are likely to cause significant impacts in many recipient ecosystems.
Nutrient remediation through denitrification is a critical service provided by marine benthic communities54. It has been proposed that Sabella could be used as a bioremediator of waste inputs from aquaculture and sewage due to its ability to remove organic matter and bacteria from the water column55,56. However, our study (and also Ross et al.28) urge extreme caution in this respect, as Sabella can compromise nitrogen cycling of sediments, and may in fact have a net negative effect on nutrient remediation. While denitrification is performed by denitrifying bacteria, the magnitude of N2 release is mediated by key infaunal biota16,57,58,59. Disruption of these key infauna by invasive habitat-structuring species has the potential to exacerbate problems associated with anthropogenic nutrient additions but has received almost no attention compared to the influence of terrestrial sediment and nutrient inputs24,60,61,62. New Zealand’s nitrogen balance worsened more than any other OECD country between 1998 and 2009, principally due to farming intensification63. Spread of Sabella throughout the Hauraki Gulf may result in greater nitrogen retention and thus potentially runaway eutrophication and increasing likelihood of hypoxic events. Compromised denitrification, increased inorganic matter content, and enhanced oxygen consumption have the potential to tip the balance of net solute and gas exchanges and cause further ecological degradation in an already eutrophic system47. To prevent further harm, resource managers may ultimately have to reduce inputs of nitrogen e.g., set lower limits of nutrient discharge, thus impacting terrestrial productivity with associated economic and social impacts.
Our results indicate a dramatic decline in denitrification efficiency at relatively low densities of Sabella and mimics, but other flux parameters had a linear response to density. While some solute flux rates (NO3, NH4) were affected by relatively low densities, N2 release, DRP release, and O2 consumption were greatly affected by worm densities above 10 per m2. The loss of these key ecosystem functions and subsequent consequences to wider ecosystem health provides significant incentive for management efforts to minimise the density of Sabella, and therefore, promote ecosystem functioning of benthic communities. There are, however, few feasible management approaches which don’t equally damage native epibiota, and the high levels of arsenic in Sabella spallanzanii tubes64 may deter native predators leaving them to spread unchecked. Given the potential cost of ongoing management over long periods, there is even greater incentive for sustained eradication efforts of bio-engineering introduced species at the early stages of incursion. Sabella has enormous potential for economic impacts to the aquaculture industry65, and our study suggests that the ecological impacts may be equally severe.
Field work for this research was performed by SCUBA divers in a subtidal (9 m depth) soft-sediment seafloor habitat in Rangitoto Channel (174.83877, 36.81350; North Island, New Zealand), approximately 10 km from where the first North Island populations of Sabella were recorded in 200945. The study site is protected from open ocean swell but has strong tidal currents (up to 0.9 m/s) and is periodically subjected to wind-driven waves.
To understand the effects of Sabella on soft-sediment ecosystem functions at the study site, live Sabella were collected from the Rangitoto Channel seafloor and transplanted into experimental plots at specific densities. To distinguish between the biological effects of live Sabella and the physical effects of the structure created by their tubes, structural ‘mimic’ Sabella were also transplanted into plots at the site. The Sabella mimics, made of flexible bungee cord, were similar to real Sabella in terms of length (25–30 cm), thickness (8 mm dia.), appearance (complete with faux feeding tentacles at the anterior end), clustering (singles, pairs, larger groupings), and movement characteristics (e.g., bending and swaying with water motion). Bungee cord was not tested for toxicity but was selected for inert materials (natural rubber and cotton). Steel pegs were used to anchor mimics and live worms in place when necessary. For the live Sabella treatments, only healthy undamaged worms with intact tubes were transplanted. Many of the Sabella collected at the study site were attached to mollusc shell fragments (sometimes several Sabella individuals per shell), and thus treatments were created by arranging naturally occurring clusters of worms into experimental plots to achieve the target densities.
In total, twenty-four 1 m2 experimental plots were established at the study site in September 2017. Plots were positioned two meters apart from one another along two perpendicular 30 m transects with a common vertex (n = 12 plots per transect). Live worms and Sabella mimics were transplanted to the plots at densities of 0, 10, 20, 35, and 50 per m2. Treatment positions were randomly interspersed along the transects. There were no mixed treatments containing both live and mimic Sabella. Experimental gradients in the densities of live and mimic Sabella were created by establishing four control plots (i.e., steel pegs only, with no live or mimic Sabella); two plots each with 10, 20 and 35 live Sabella; two plots each with 10, 20 and 35 mimic Sabella; and four plots each with 50 live and 50 mimic Sabella. Extra replicates in the end member treatments (i.e., four replicates each in the “0” and “50” treatments, rather than two each) limited the influence of outlying data points in these treatments and therefore provided greater statistical power. Given the potential for density-dependent mortality over time in the live Sabella treatments, the design also increased the likelihood of an even distribution of densities of live worms across the gradient at the end of the experiment when ecosystem functions and various environmental metrics were assessed.
End of experiment sampling
In March 2018, approximately six months after establishing experimental treatments, plots at the study site were re-sampled. The aim was to quantify the effects of Sabella on benthic rates and processes (assessed by examining fluxes of dissolved solutes across the sediment-water interface). and on sediment and benthic community characteristics that are known to interactively influence those rates and processes.
Fluxes of dissolved oxygen, elemental nitrogen (N2) and inorganic N and P were quantified using benthic incubation chambers, as described by Lohrer et al.16,66. One square aluminium chamber base (50 cm × 50 cm, wall 15 cm high) was positioned in the centre of each experimental plot. Chamber bases were pressed ~5–7 cm deep into the sediment, enclosing a 0.25 m2 patch of sediment and a known number of live or mimic Sabella. A DOpto dissolved oxygen logger sampling at 1 minute intervals and a SeaBird Electonics water pump (pulsed, non-directional stirring) was positioned on the interior edge of each base.
The next morning, watertight Perpex lids were clamped onto each chamber base to initiate incubations, sealing ~30 L of bottom seawater in with enclosed sediments in each plot. Opaque shade clothes were used to cover the chambers (i.e., dark incubations in all plots). Although DO was measured at 1-minute intervals with the loggers, two 60 ml water samples were also collected manually from each chamber (through syringe-activated sampling ports) to track changes in DO and other dissolved solutes (elemental nitrogen, ammonium, nitrate + nitrite, and phosphate) during the 3 hour incubation period.
Immediately upon surfacing, the concentration of DO in one of each pair of water samples was assessed using a handheld dissolved oxygen meter (YSI ProODO). The sample was then filtered across 0.8 mm Whatman glass fibre filter and stored frozen until later analysis of dissolved inorganic N and P (Astoria-Pacific 300 series segmented flow auto-analyser with detection limits of 1 mg/m3 for N and P). The second sample was decanted into triplicate 15 ml glass ‘exetainers’, which were filled to overflowing, preserved with 1 drop of mercuric chloride (HgCl2), and capped without any air bubbles or headspace. The capped exetainers were stored in a water bath (at 1–4 C below the temperature of ambient bottom water at the time of sample collection) until analysis for N2 gas concentration and [N2]/[Ar] ratios using membrane inlet mass spectrometry (MIMS67).
In all cases, fluxes of solutes were calculated as concentration change during incubation (µmol/L) times chamber volume (30 L), divided by elapsed incubation time (h) and area of sediment enclosed (0.25 m2).
At the end of the chamber incubations and after the lids had been lifted, two small sediment cores (3 cm internal diameter, 2 cm deep) were collected from each experimental plot: one for sediment grain size and organic matter content analysis68,69, the other for sediment chlorophyll a and phaeopigment content70. Two larger cores (12 cm internal diameter, 10 cm deep) were collected from each plot to sample benthic macrofauna communities. Macrofaunal samples were sieved across a 0.5 mm mesh screen; preserved in 70% isopropyl alcohol, stained with Rose Bengal, sorted, and identified to the lowest practicable level. All fauna >5 mm remaining in the chamber bases, including live Sabella and the Sabella mimics, were collected in mesh bags and brought to the surface for enumeration. Biomass (blotted wet weight) of the transplanted S. spallanzanii present in each chamber, and biomass of the organisms attached to the live and mimic Sabella, was also quantified.
Various univariate and multivariate statistical procedures were used to assess the influence of Sabella, six months after establishing experimental gradients in the densities of live worms and mimics. The effects of manipulated variables and environmental co-variables (grain size, OM content, sediment pigments, macrofauna) on fluxes of dissolved solutes, oxygen, ammonium, nitrate, total DIN (dissolved inorganic nitrogen), phosphate and N2 were analysed. Although gradients in the densities of live and mimic Sabella were categorical at the 1 m2 plot scale at the time of experimental setup (i.e., 0, 10, 20, 35 and 50 worms/mimics per plot in September 2017), the 0.25 m2 chambers (n = 24) did not enclose all of the worms/mimics transplanted into a plot, and mortalities were expected to occur in some of the live Sabella treatments. Therefore, densities of worms/mimics present in the chambers at the time of sampling (March 2018) were used as continuous predictor variables in analyses of flux variables. Multiple linear regression models were constructed in RStudio71 to partition the sources of variation in the solute flux response variables. Predictor variables included the experimental treatment (Sabella vs. mimics), density of worms (continuous predictor), sediment chlorophyll a content, sediment phaeopigment content, sediment organic content, and the contribution the large and small grainsize fractions to sediment composition (gravel and clay). Model predictor selection was performed by stepwise AIC selection criteria (forwards and backwards). Model assumptions were checked using fitted vs residual, QQ, and scale location plots. Diagnostics showed linear relationships (fitted vs residuals), residuals were normally distributed except for a few outliers (QQ), and data showed homoscedasticity (scale-location plots).
Alongside flux rates, the denitrification efficiency was also calculated to assess the relationship between DIN diverted away from benthic process through direct ammonia excretion (Ross et al.28). DE (%) was defined as the proportion of N fluxed into the water column as N2 compared with the total dissolved inorganic N (DIN; NH4, and NO3) released into the water column and was calculated from the following:
where N2 is the number of moles of N fluxed into the water column as N2, and DIN is the number of moles of DIN fluxed into the water column (Ross et al.28).
Distance based linear models (DistLM) were used to examine the relative influence of several explanatory environmental variables and community composition on flux rates. Flux rates were normalised and resemblance matrices were calculated using Euclidean distance similarities to produce a multivariate metric of flux profiles (i.e., the combined flux response of each replicate plot). The explanatory variables were; above sediment biomass; sediment chlorophyll a content; sediment phaeopigment content; sediment organics content; and proportion of seven grainsize fractions in the sediment. Distance based linear models were performed using the backward selection method and the AIC selection criteria. Flux rates were plotted using distance-based redundancy analysis (db-RDA), with the species and environmental variables driving variation plotted separately as vector overlays. The influence of the experimental factors, density and treatment on flux profiles were analysed using permutational analysis of variance (PERMANOVA). Both treatment (2 factors ‘Sabella’, and ‘Mimic’), and density (5 factors; 0, 10, 20, 35, and 50) were treated as fixed factors. “Analysis was performed on a Euclidean distance” resemblance matrix and used a reduced model with 999 permutations. All multivariate analyses were performed using the statistical package PRIMER 7.
The datasets generated during and/or analysed during the current study are available from the corresponding author on reasonable request.
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The field component of this research would not have been possible without the assistance of Samantha Parkes, Rod Budd and Mathew Cameron. We also thank Andrew Marriner, Kelly Carter and Katie Cartner for analysing nutrient samples, and for processing and analysing sediment samples. We also acknowledge MPI- New Zealand Biosecurity (Kathy Walls) for processing permits for the transplanting of Sabella spallanzanii. This research was funded by the New Zealand Ministry for Business, Innovation and Employment (MBIE) grant number C01X1511.
The authors declare no competing interests.
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Tait, L.W., Lohrer, A.M., Townsend, M. et al. Invasive ecosystem engineers threaten benthic nitrogen cycling by altering native infaunal and biofouling communities. Sci Rep 10, 1581 (2020). https://doi.org/10.1038/s41598-020-58557-8