Genetic manipulation of the oviductal cells. In (a), comparison of human and dog TP53 proteins, showing 79% homology in their sequence. (b), CRISPR-Cas9 mediated editing of TP53 gene in the oviduct-on-a-chip with a representation of all Insertions and Deletions (Indels) encountered by editing with both sgRNA 1 and 2 simultaneously. (c) depiction of the different Indels percentage. (d) section of Western blot for the TP53 gene in the WT and KO cells (full gel image and negative control presented as Supplementary Fig. S1). And (e) TP53 protein content normalized to B-actin (n = 4, paired sample t-tests, p = 0.024).