Oviduct-on-a-chip model, leakage test and epithelial cell culture. In (a) different parts of the device with bottom (basolateral, pink) and top (apical, blue) compartments, and the assembled microfluidic device with a porous membrane in the middle; each device comprises two independent chambers. Right graphic shows a transverse cut of the chamber, indicating the oviductal epithelium growing on the top of the porous membrane in the apical compartment. In (b) leakage test showing the percentage of devices not leaking as a function of the applied shear stress; note lower shear stress used in brain- and liver-on-a-chip platforms41,42. In (c), immunofluorescence with reconstructed side-views of TP53WTcells grown in the oviduct-on-a-chip for 2 weeks showing nuclei (Hoechst33342, blue), actin filaments (phalloidin, green), and cilia (acetylated alpha tubulin, red). In (d), relative mRNA expression of TP53, Ki67 and PAX8 of fresh collected cells and non-edited cells cultured in the oviduct-on-a-chip for 2 weeks. Scale bar = 25 µm.