Heterologous expression of xanthophyll esterase genes affects carotenoid accumulation in petunia corollas

The majority of carotenoids in petals are xanthophylls and most of these xanthophylls are esterified with fatty acids. Although petunia (Petunia x hybrida) is an important ornamental plant, it cannot accumulate enough carotenoids to have deep-yellow flowers. Our previous study suggested that low esterification activity causes low carotenoid accumulation in petunia corollas. Here, we introduced xanthophyll esterase (XES) from the petals of Ipomoea obscura, tomato (Solanum lycopersicum), and marigold (Tagetes erecta) into a pale-yellow-flowered cultivar of petunia to see whether these affect carotenoid accumulation in petunia corollas. Carotenoid contents and the proportions of esterified xanthophylls were elevated in the corollas of XES-overexpressing (XES-OX) transformants. Expression analysis showed that the transcript levels of endogenous carotenoid biosynthetic genes, which included geranylgeranyl diphosphate synthase 2, ζ-carotene desaturase, and lycopene β-ring cyclase in corolla tubes were upregulated in XES-OX plants. In addition, we discovered a difference in the composition of esterified xanthophylls among XES-OX plants, which may be caused by differences in the substrate specificity of their respective XESs. We conclude that esterification is an important process for carotenoid accumulation and XES is a useful tool for the quantitative and qualitative control of carotenoid accumulation in petals.

the cells to accumulate massive amounts of carotenoids. Therefore, we consider that esterification may enhance xanthophyll accumulation and cause deeper yellow coloration in flowers. Ariizumi et al. identified a tomato (Solanum lycopersicum L.) mutant, pale yellow petal 1 (pyp1), which lacks esterified xanthophylls in flowers 17 . Complementation of the PYP1 gene into the pyp1 mutant recovered esterified xanthophylls to the level of wild-type (WT) plants, indicating that PYP1 functions as a xanthophyll esterase (XES). Furthermore, since the pyp1 mutant showed abnormal chromoplast development, they presumed that accumulation of esterified xanthophylls is important for normal chromoplast development. They proposed that PYP1 might be a key enzyme responsible for the high carotenoid accumulation in petals of various plants, including tomato.
Petunia (Petunia x hybrida) is an important ornamental plant and an excellent model plant for investigating floral pigmentation 23 . Although there are no deep-yellow-flowered petunia cultivars, there are pale-yellow-flowered cultivars that accumulate a small amount of carotenoids in their corollas 24,25 . To determine why petunias are unable to accumulate enough carotenoids to produce deep-yellow flowers, we previously compared carotenoid profiles and expression of carotenoid metabolic genes between pale-yellow-flowered petunia and deep-yellow-flowered calibrachoa (Calibrachoa x hybrida), a close relative of petunia 26 . We showed that the carotenoid contents and proportions of esterified xanthophylls in the corollas of petunia were significantly lower than in calibrachoa. In addition, we assumed petunia XES to have low substrate specificity for trans-xanthophylls, which are more abundant than cis-xanthophylls in corollas. These results suggest that a major reason for low carotenoid accumulation in petunia corollas is low esterification activity.
Here, we made transgenic petunia plants overexpressing XES from a yellow-flowered variety of Ipomoea obscura (L.) Ker Gawl., tomato, and marigold (Tagetes erecta L.). Petals of these plants had large amounts of esterified trans-xanthophylls with various compositions, suggesting that the introduced XESs had high esterification activity in respect of trans-xanthophylls and different substrate specificities 5,15,17 . We demonstrated that overexpression of these XES genes promoted the esterification of xanthophylls, development of carotenoid-lipoprotein sequestering substructures in chromoplasts, and overexpression of three endogenous carotenoid biosynthetic genes, resulting in an increase in total carotenoid content in the petunia corollas. Additionally, we showed that substrate specificity of XES affected the carotenoid profiles in the corollas of the transgenic plants.

Results
Isolation of full-length cDNA of XES from I. obscura and marigold and production of XESoverexpressing petunia plants. We isolated full-length cDNA sequences of XES from petals of I. obscura (IoXES) and marigold (TeXES). The deduced amino acid sequences covered 721 aa (IoXES) and 709 aa (TeXES); that of tomato PYP1 (SlXES) showed 59% similarity with that of IoXES and 55% similarity with that of TeXES. Tomato and petunia belong to the same Solanaceous family and SlXES and PhXES showed high similarity in the phylogenetic tree. In addition, IoXES and TeXES belong to different families, but the similarity of these 4 XESs was relatively high. An XES ortholog of Arabidopsis, known to function as phytyl ester synthase 1, and these 4 XESs were classified into different clades (Fig. S1).
We then introduced constructs overexpressing IoXES, SlXES, or TeXES into petunia 'California Girl' , which has pale yellow flowers, and obtained 6, 10, and 8 independent lines of transformants (OX), respectively. We chose four IoXES-OXs, five SlXES-OXs, and five TeXES-OXs for further analyses. Each group consisted of one transformant whose corolla color was unchanged (#1 transformant in all lines) and others whose corollas were deeper yellow than the WT plants (Fig. 1A).

Comparison of carotenoid profiles between XES-OX and WT plants.
In corolla tubes and limbs ( Fig. 1B) and leaves, we compared carotenoid content between the WT and XES-OX plants (Figs. 1C,D and 2). All transgenic plants had significantly higher total carotenoid content than WT plants except for the tubes and limbs of the #1 plants (IoXES-OX #1, SlXES-OX #1, and TeXES-OX #1), the tubes of IoXES-OX #2 and #3, and the limbs of IoXES-OX #3 and #4 (Fig. 1C). TeXES-OX #5 had the highest carotenoid content in both tubes (3.11 × the WT value) and limbs (2.90×). Leaves of the SlXES-OX #5 accumulated significantly more carotenoids than those of IoXES-OX #4, TeXES-OX #5, and WT plants. HPLC analysis detected esterified xanthophylls in the non-saponified samples at a retention time of approximately 20 to 30 min in all limbs and tubes of XES-OX and WT plants tested (see examples in Fig. 2). The total amounts of esterified xanthophylls were significantly higher in all XES-OX plants except for #1 plants than in WT plants (Fig. 1C). Increase in esterification percentage was mostly associated with increase in total carotenoid content in the transgenic plants except in the limbs of IoXES-OX #3 and #4 (Fig. 1C,D). As observed for total carotenoid content, the tubes and limbs of the #1 plants showed a similar or lower esterification percentage than that of WT plants; the one exception was the limbs of IoXES-OX #1, which showed a significantly higher esterification percentage than WT. No esterified xanthophylls were detected in all leaves (Fig. 1C).
We tested the relationship between esterification percentage and total carotenoid content by Pearson's correlation analysis (Fig. S3). There was a weak positive correlation between esterification percentage and total carotenoid content in all limb and tube samples (r = 0.396, p = 0.030; WT was included in all analyses). The trends differed between IoXES-OX and SlXES-OX/TeXES plants. In SlXES-OX and TeXES-OX plants, there was a strong positive correlation between esterification percentage and total carotenoid content (SlXES-OX: r = 0.829, p < 0.001; TeXES-OX: r = 0.911, p < 0.001). In IoXES-OX plants, there was a moderately positive correlation between esterification percentage and total carotenoid content in all samples (r = 0.459, p = 0.182).
We detected esterified and free-form xanthophylls and β-carotene by HPLC (Fig. 2) and then compared the content and the esterification percentage of each carotenoid component between OX and WT plants (Fig. 3). The trends in content and esterification percentage of the various carotenoid components depended on the origin of the introduced XES, as outlined below.
In IoXES-OX plants #3 and #4, contents of zeaxanthin + antheraxanthin and β-cryptoxanthin were significantly higher than those in WT plants, whereas in all IoXES-OX plants, the contents of (all-E)-neoxanthin, (all-E)-violaxanthin, (9′Z)-neoxanthin, and (9Z)-violaxanthin were significantly lower than or not significantly different from those in WT plants. In all IoXES-OX plants except #1, percentages of xanthophyll esterification tended to be higher than those in WT plants. The contents of zeaxanthin + antheraxanthin and β-cryptoxanthin were closely associated with their esterification percentages.
We tested the relationship among the expression of each transgene, esterification percentage, and total carotenoid content in tubes by Pearson's correlation analysis. There was a very strong positive correlation between IoXES expression and esterification percentages (r = 0.965, p = 0.008; WT was included in all analyses). In contrast, there was a moderate negative correlation between IoXES expression and total carotenoid content (r = −0.496, p = 0.395). The result showed that the IoXES expression in IoXES-OX plants was closely associated with esterification percentages but not with carotenoid content. There was a very strong positive correlation between SlXES expression and esterification percentages (r = 0.864, p = 0.027), SlXES expression and total carotenoid content (r = 0.956, p = 0.003), TeXES expression and esterification percentages (r = 0.839, p = 0.037), and TeXES expression and total carotenoid content (r = 0.816, p = 0.048). The esterification percentage and total carotenoid content in SlXES-OX and TeXES-OX plants were significantly associated with transgene expression.
We then analyzed the expression levels of 20 endogenous carotenoid metabolic genes in the tubes and leaves of XES-OX and WT plants (Figs. 5 and 6). Among the genes that encode carotenoid biosynthetic enzymes, Among the carotenoid catabolic genes, we analyzed expression of carotenoid cleavage dioxygenase (CCD) 1 and 9-cis-epoxy carotenoid dioxygenase (NCED) 2, but not CCD4a or CCD4b, because we have previously clarified that expression of CCD4a is not detected at all and expression of CCD4b is not associated with carotenoid accumulation in corollas of 'California Girl' , the petunia cultivar used in this study (Kishimoto et al., 2018). NCED2 expression was lower in all XES-OX plants than in WT plants; this trend was significant for all plants except IoXES-OX #1. NCED2 expression tended to be inversely proportional to total carotenoid content. CCD1 expression showed no significant difference between XES-OX and WT plants, except for the significantly higher expression in IoXES-OX #2.
Plastid ultrastructure in the corollas of WT and XES-OX plants. By   www.nature.com/scientificreports www.nature.com/scientificreports/ used in this study (Fig. 1). In WT plants, plastids in mesophyll and epidermal cells had several plastoglobules (globular-type carotenoid-lipoprotein-sequestering substructures) with high electron density inside the granules and a thylakoid membrane with a few grana stacks (Fig. 6A,B). In the plastids in TeXES-OX #5 mesophyll cells, the thylakoid membrane was less clear than those in WT mesophyll cells (Fig. 6C). In the plastids in TeXES-OX #5 epidermal cells, plastoglobules had lower electron density inside the granules and the thylakoid membrane was hardly visible (Fig. 6D).

Discussion
We previously reported that trans-xanthophylls in petunia corollas have lower esterification percentages than cis-xanthophylls, which suggests that PhXES has low substrate specificity for trans-xanthophylls 26 . Here, we introduced XES genes from I. obscura, tomato, and marigold to increase the proportion of esterified xanthophylls in petunia corollas. Petal of these plants accumulate large amounts of trans-xanthophylls with various compositions 5,15,17 , which suggest that their XESs have high esterification activity in respect of trans-xanthophylls. We previously compared the amino acid sequences of XES in tomato and petunia and found that there are differences in the sequence in the presumed important domains for enzyme activity 26 . We assume that those differences affect the substrate specificity and esterification activity of SlXES and PhXES. We hypothesized that the difference in composition of esterified xanthophylls in I. obscura, tomato, and marigold is caused by differences in the substrate specificity of their respective XESs and that by introducing XES genes from these species into petunia, we could potentially esterify different types of trans-xanthophylls. We showed that the expression of introduced XES genes from I. obscura, tomato, and marigold affected the proportions of esterified xanthophylls in the tubes and limbs of XES-OX corollas (Figs. 1C and 4).
There was a strong positive correlation between esterification percentage and total carotenoid content in TeXES-OX and SlXES-OX plants (Fig. S3). In IoXES-OX plants, esterification percentages tended to be higher than in the SlXES-OX and TeXES-OX plants; however, the correlation between the esterification percentage and carotenoid content was weaker than that in TeXES-OX and SlXES-OX plants. In addition, some IoXES-OX plants had lower carotenoid content than WT plants despite high esterification percentages. It is necessary to investigate why IoXES-OX plants had lower efficiency for xanthophyll ester accumulation than SlXES-OX and TeXES-OX plants.
There was a strong positive correlation between the expression level of all the transgenes and esterification percentages, and the heterologous expression of XES caused an increase in the esterification percentage. However, we considered that xanthophylls in XES-OX plants were not efficiently esterified. We used the 35 S promoter to drive the constitutive expression of transgenes and the expression level of the transgenes in tubes was nearly 10 times greater than that of the endogenous XES gene, but the maximum esterification percentage was only 66.5% in the tube of IoXES-OX #4. If the esterification percentage is increased up to 100% as in marigold, a bright yellow corolla with a total carotenoid content of approximately 200 µg·gFW −1 is expected, according to the regression equation obtained in this study (Fig. S3). However, as it does not reach such a high percentage, there are likely to be other factors that further increase the esterification percentage and future research is needed to investigate the other factors.
Introduced XES genes were expressed in the leaves of transformants, but xanthophylls contained in the leaves were not esterified at all (Fig. 1C). Ytterberg et al. performed proteome analysis in plastoglobules of red pepper and Arabidopsis and detected the amino acid sequence of an XES ortholog (homolog of Accession no. At1g54570) and other carotenogenic enzymes 27 . It is presumed that XESs catalyze esterification reactions in hydrophobic conditions inside plastoglobules, in the same way as other carotenoid biosynthetic enzymes in non-green tissues, and chloroplast structures in leaves are inadequate for XES activity.
Petals of the yellow-flowered variety of I. obscura predominantly accumulate as (all-E)-β-cryptoxanthin, most of which is present in the esterified form 5 . In the petals of marigold, nearly 100% of carotenoids are esterified (all-E)-lutein 15   www.nature.com/scientificreports www.nature.com/scientificreports/ of (all-E)-neoxanthin and (all-E)-violaxanthin did not increase substantially in SlXES-OX plants compared with WT. Furthermore, esterified zeaxanthin + antheraxanthin was significantly and dramatically increased, even though zeaxanthin and antheraxanthin are detected in trace amounts in tomato petals. We consider it likely that one factor that prevented the accumulation of esterified neoxanthin and violaxanthin in the SlXES-OX plants was the low biosynthesis of these xanthophylls. Our expression analysis showed that the transcript level of the zeaxanthin epoxidase (ZEP) was low in the tubes of XES-OX and WT plants. Low ZEP activity might have prevented the conversion of zeaxanthin to violaxanthin, which resulted in increased accumulation of zeaxanthin. The effect of low ZEP expression on carotenoid accumulation is also discussed below. In the TeXES-OX plants, unlike the IoXES-OX and SlXES-OX plants, a slight increase in violaxanthin and neoxanthin in both trans-and cis-forms was observed. This may be because TeXES mainly targets lutein, an α-carotene derivative. As IoXES and SlXES mainly target β-carotene derivatives, biosynthetic intermediates, such as β-cryptoxanthin and zeaxanthin, may be converted to the ester form before reaching neoxanthin and violaxanthin. In contrast, it is likely that TeXES has a lower effect on β-carotene derivatives than the other two XESs, and neoxanthin and violaxanthin are increased regardless of low ZEP activity.
We presume that esterified xanthophylls may not continue in the steps of carotenoid biosynthesis because of their sequestration into plastoglobules or the substrate specificity of carotenoid biosynthetic enzymes, such as by hydroxylation and epoxidation enzymes. This means that xanthophylls preferentially esterified by XES may be dominantly accumulated. Here, we showed that IoXES plants preferably esterified β-cryptoxanthin and enhanced its accumulation in petunia corollas, which originally accumulate only a trace amount. β-Cryptoxanthin, which is formed by the hydroxylation of one of the β-rings of β-carotene by CHYB, has attracted much attention as a functional and nutritional component [28][29][30] . When humans ingest esterified β-cryptoxanthin, an ester bond is cleaved during digestion. The concentration of free β-cryptoxanthin in the blood after ingestion increases regardless of whether it was an ester form or free form. Therefore, the presence or absence of an ester bond does not affect the function of nutrient components 31 . However, only a few plants are known to accumulate β-cryptoxanthin 32 , presumably because most plants have XES orthologs with no or low esterification activity for β-cryptoxanthin. In plants without this activity, free-form β-cryptoxanthin is expected to be continuously hydroxylated to form zeaxanthin. In contrast, in plants with high levels of this activity, such as IoXES-OX plants and I. obscura, β-cryptoxanthin can be esterified, which prevents it from being converted to zeaxanthin. Therefore, we propose that IoXES may be a powerful tool for enhancing the accumulation of β-cryptoxanthin in flowers and fruits containing xanthophylls.
In our comparison of the ultrastructure of plastids (the carotenoid storage organelles) between TeXES-OX #5 and WT plants (Fig. 6), we observed that the plastoglobules in both epidermal and mesophyll cells of TeXES-OX #5 had lower electron densities than those in WT plants. Ariizumi et al. previously reported the same tendency: plastids in petals of WT tomato plants are filled with numerous plastoglobules with low electron density, whereas those in pyp1 plants, which lack XES activity, contain small and high-electron-density plastoglobules 17 . We assume that, in XES plants, an increase in the accumulation of esterified xanthophylls into plastoglobules causes a decrease in their electron density.
There is increasing evidence that feedback and feed-forward mechanisms play important roles in the regulation of the carotenoid biosynthetic pathway 33,34 . Recently, in tomato, the interaction between various substrates and enzyme activities has become apparent by introducing heterologous carotenoid biosynthetic genes www.nature.com/scientificreports www.nature.com/scientificreports/ into carotenoid deficient mutants, e.g., tangerine (a loss-of-function mutant of carotenoid isomerase) and old gold crimson (a loss-of-function mutant of fruit-specific lycopene β-cyclase) 35,36 . For example, the introduction of the bacterial desaturase/isomerase gene (CRTI) into tomato deficient mutants reveals that the decrease in phytoene, which is a substrate for CRTI, causes negative feedback regulation and results in the decreased expression of PSY. Simultaneously, it became clear that the increase in trans-lycopene, a CRTI product, also causes feed-forward regulation that increases the expression of lycopene cyclases 35 . It is speculated that the reason cultivated carrots accumulate a higher amount of α-carotene than wild carrots is due to the positive feedback regulation of PSY expression caused by the loss-of-function mutation of cytochrome P450-type β-ring hydroxylase (CYP97A3) 37 . Among the endogenous carotenoid metabolic genes tested in this study (Fig. 5), the levels of the transcripts of the carotenoid biosynthetic genes (GGPS2, ZDS, and LCYB) were significantly higher in most of the XES-OX plants that had higher carotenoid content than those in WT plants. The putative carotenoid biosynthetic pathway in the corollas of XES-OX plants is shown in Fig. 7. We previously showed that the expression of PSY1 and lycopene ε-ring cyclase in petunia cultivars is significantly higher in pale yellow corollas than in white corollas 13 . We also showed that the higher carotenoid content in tubes than that in limbs in both white and pale-yellow corollas is associated with higher expression of GGPS1, PSY1, carotenoid isomerase, lycopene ε-ring cyclase, and cytochrome P450-type β-ring hydroxylase. The genes that showed higher expression in the XES-OX plants than that in the WT plants in this study are different from those that showed higher expression in tubes than in limbs in the previous study 26 . Therefore, we presume that the mechanism by which carotenoid accumulation is controlled in corollas differs between XES-OX plants and petunia cultivars. The increased expression of genes in XES-OX plants was likely to be induced by feedback from the accumulation of esterified xanthophylls or overall carotenoids. www.nature.com/scientificreports www.nature.com/scientificreports/ In deep-yellow-flowered calibrachoa, the content of β-carotene is higher than that in WT petunia 26 , asas we observed in SlXES-OX and TeXES-OX plants. The expression levels of isopentenyl diphosphate isomerase, PSY1, LCYB, cytochrome P450-type ε-ring hydroxylase, ZEP, and XES are higher in calibrachoa than those in petunia. A higher expression level of LCYB was observed in both calibrachoa 26 and XES-OX plants (Fig. 5) than that in WT petunia plants, which suggested that LCYB is a key enzyme in the synthesis of β,β-carotenoids (β-carotene and its derivatives) in the corollas of calibrachoa and petunia. In the SlXES-OX plants, the consistently higher contents of β,β-carotenoids, such as β-carotene and zeaxanthin + antheraxanthin, might be due to the higher expression of LCYB in these plants than that in IoXES-OX and TeXES-OX plants (Figs. 3 and 5). Here, we propose that both the increased percentage of xanthophyll esterification and the enhanced carotenoid biosynthetic gene expression cause an increase in the carotenoid accumulation in XES-OX plants. XES-OX plants did not reach the carotenoid level observed in calibrachoa corollas, perhaps because the expression of ZEP was lower in both XES-OX and WT plants than that in calibrachoa (Figs. 1C, 5 and 6) 26 . PhXES and SlXES are expected to have a higher esterification activity in regard to neoxanthin and violaxanthin than that of the other xanthophylls because of the high esterification percentage of xanthophylls in petunia 26 and tomato 17 . However, SlXES-OX plants cannot utilize the ability to esterify neoxanthin and violaxanthin due to the low accumulation of these two components. If ZEP expression was to be increased in the corollas of XES-OX and WT plants, we consider it likely that the biosynthesis of neoxanthin and violaxanthin would be elevated and that the esterified forms of neoxanthin and violaxanthin would increase. This increase in xanthophyll esters would further promote biosynthetic gene expression and the development of plastoglobules in chromoplasts, which would result in a further increase in total carotenoid accumulation.
Our expression analysis of carotenoid catabolic genes showed that xanthophyll esterification was also associated with NCED2 expression in XES-OX plants. NCED2 had a significantly lower expression in XES-OX plants than that in the WT plants and total carotenoid content of XES-OX plants tended to be inversely proportional to NCED2 expression levels (Figs. 1C and 5). NCED is localized in chloroplast thylakoid membranes and cleaves (9Z)-violaxanthin and (9′Z)-neoxanthin to xanthoxin, which is a precursor of abscisic acid 38,39 . However, the neoxanthin and violaxanthin content in IoXES-OX and SlXES-OX plants was not higher than those in WT plants (Fig. 3). It is possible that NCED2 is unable to cleave violaxanthin and neoxanthin in the plastoglobules of chromoplast and that the expression level of NCED2 is independent of the amount of violaxanthin and neoxanthin in the corollas.

Conclusion
The main effects that introducing XES genes from different species had on petunia corollas were promotion of xanthophyll esterification and increase in LCYB expression, possibly caused by feedback regulation. These multiple effects likely caused the elevated carotenoid accumulation in corollas of XES-OX plants. Only a few attempts at manipulation of carotenoid accumulation in petals by genetic engineering have been reported. Watanabe et al. introduced a set of carotenoid biosynthetic genes into white-flowered Ipomoea nil with the intention of producing yellow flowers 40 , since the expression of many carotenogenic genes is suppressed in its petals 5 , but although the carotenoid content in the petals of the transformants increased to ~1 μg/g FW, it was insufficient to make the flowers visually yellow. These results indicate that carotenoid biosynthetic activity has limited effect on carotenoid accumulation in petals. A number of genes other than carotenogenic genes influence carotenoid accumulation, making metabolic engineering of carotenoids difficult. Here we showed that introducing a single gene, XES, could clearly change the petal color of petunia (Fig. 1A). We also showed that the substrate specificity of introduced XESs had a marked effect on the carotenoid composition in the transformants. We suggest that substrate specificity is a reason for the diverse carotenoid profiles among different plant species. To control the accumulation of specific carotenoids, further study of the relationship between XES activity and carotenoid composition is needed.

Methods
Plant materials. Transgenic plants were produced from the pale-yellow-flowered petunia cultivar 'California Girl' . Seedlings were grown on Murashige-Skoog agar medium with half-strength minerals at 25 °C under a 16-h light/8-h dark photoperiod with fluorescent light (120 μmol m −2 s −1 ). The fully-grown seedlings were applied to Agrobacterium-mediated transformation or transferred to soil and grown in a greenhouse at the Institute of Vegetable and Floriculture Science, NARO (Tsukuba, Japan). The second to fifth flowers, in order of flowering time, in each plant were sampled from 9 to 11 a.m. on the flowering day. Three corollas were harvested and divided into limbs and tubes. They were further divided vertically; one used for carotenoid analysis and the other for gene expression analysis. Mature leaves were sampled and stored at −80 °C.
For isolation of genes, a bright-yellow-flowered variety of I. obscura 'Keniaki' , tomato 'Mini Carol' , and orange-flowered marigold 'Orange Lady' were grown in greenhouses. Petals from fully opened flowers were sampled and stored at −80 °C until use.
Cloning of full-length XES genes. We isolated full-length cDNA sequences of XES, an ortholog of tomato PYP1, from I. obscura and marigold by 5′ RACE and 3′ RACE as described by Kishimoto et al. 13 , using the primer sequences shown in Table S1. We isolated the full-length SlXES cDNA sequence by conducting RT-PCR using the conditions described by Kishimoto et al. 26 and 5′-and 3′-end primers designed from the published sequence (acc. no. XM_004230093; Table S1). The full-length cDNA sequences of IoXES (LC335777) and TeXES (LC335776) are available in the DDBJ/EMBL/GenBank nucleotide sequence database.
The deduced amino acid sequences of the XES genes identified in this study were compared with those in the database by using the neighbor-joining method and bootstrap analysis (1000 replicates) in PHYLIP software 41 and were mapped in Treeview v. 1.6.6 software 42 .