Table 2 Set of primers designed for the alanine-tRNA ligase gene from Acanthamoeba spp.

From: Core gene-based molecular detection and identification of Acanthamoeba species

Primer nameForward/ReverseSequence 5′-3′Length (nucleotides)Amplicon size (bp)
Lig1_FForwardCTTCAAGGAGGAGGCCAT18684 bp
Lig1_RReverseCTGCTTGCCGTAKCGCAC18 
Lig2_FForwardGAGAACTTCTGGGAGATGGG20783 bp
Lig2_RReverseCCTTCTCCTCGGCCATGAG19 
Lig3_FForwardCTCTGCGGTGGTACCCAC18472 bp
Lig3_RReverseCGGATGGCCTTGATGGC17 
  1. bp, base pair.