A novel method for biosynthesis of different polymorphs of TiO2 nanoparticles as a protector for Bacillus thuringiensis from Ultra Violet

Bacillus thuringiensis (Bt) were used for biosynthesis of amorphous TiO2 converted to distinct polymorphs (anatase, rutile, mix) under different temperature conditions. Characterizations of TiO2 nanoparticles were performed by using X-ray diffraction spectroscopy (XRD), Fourier-transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM) and, energy-dispersive X-ray spectroscopy (EDX) analysis. Stability of five formulations under ultraviolet (UV) radiation with spore viability and mortality test on Ephestia kuehniella Zeller larvae were investigated. TiO2(mix) showed the highest viabilities of 79.76% after exposure to ultraviolet (UVA385 nm), while viabilities of non-protected spores under these conditions were 41.32%. The mortality of TiO2(mix), TiO2(anatase), TiO2(rutile), TiO2(amorphous) and free spore formulations on second-instar larvae of Ephestia kuehniella were 73.76%, 71.24%, 57.12%, 51.32%, and 50.32%, respectively on the 10th day of the experiment. The obtained results suggest that TiO2(amorphous) does not increase Bt resistance, but both phases of TiO2 nanoparticles synthesized (anatase and rutile) through the Bacillus thuringiensis and phase mixture can increase the persistence of Bt to the UV light. Furthermore, the combination of both crystalline phases of TiO2(mix) has the highest performance in improving the Bt resistance.

A novel method for biosynthesis of different polymorphs of TiO 2 nanoparticles as a protector for Bacillus thuringiensis from Ultra Violet elham Jalali 1,2 , Shahab Maghsoudi 1* & ebrahim noroozian 1 Bacillus thuringiensis (Bt) were used for biosynthesis of amorphous TiO 2 converted to distinct polymorphs (anatase, rutile, mix) under different temperature conditions. Characterizations of TiO 2 nanoparticles were performed by using X-ray diffraction spectroscopy (XRD), Fourier-transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM) and, energy-dispersive X-ray spectroscopy (EDX) analysis. Stability of five formulations under ultraviolet (UV) radiation with spore viability and mortality test on Ephestia kuehniella Zeller larvae were investigated. TiO 2 (mix) showed the highest viabilities of 79.76% after exposure to ultraviolet (UVA385 nm), while viabilities of non-protected spores under these conditions were 41.32%. The mortality of TiO 2 (mix), TiO 2 (anatase), TiO 2 (rutile), tio 2 (amorphous) and free spore formulations on second-instar larvae of Ephestia kuehniella were 73.76%, 71.24%, 57.12%, 51.32%, and 50.32%, respectively on the 10th day of the experiment. The obtained results suggest that tio 2 (amorphous) does not increase Bt resistance, but both phases of TiO 2 nanoparticles synthesized (anatase and rutile) through the Bacillus thuringiensis and phase mixture can increase the persistence of Bt to the UV light. Furthermore, the combination of both crystalline phases of tio 2 (mix) has the highest performance in improving the Bt resistance.
The most widely used microbial insecticides are those based on the bacterial pathogen Bacillus thuringiensis (Bt) used as a prosperous biological insecticide and an alternative to chemical pesticides for many years. It has been applied widely in pest management in forestry, agriculture and public health because of its safety to humans, animals, and the environment 1 . Bt not only is harmless to human and non-targeted insects, but also is an entomopathogen that produces insecticidal crystal proteins that are toxic for lepidopteran, dipteran, or coleopteran larvae 2 . A significant pest in the milling industry is the Mediterranean flour moth (Ephestia kuehniella Zeller (Lepidoptera: Pyralidae)) that is convenient, particularly due to available, thermal resistant, and easy to breed 3 . Entomopathogens lose their persistence when exposed to the sunlight in the field. Despite the fact that insecticides containing Bt can be very useful for insect control in a variety of situations, the short persistence of Bt agents after the application has become a primary influencing factor for its subsequent development 4 . Variable environmental factors, such as rain, UV radiation, and temperature, leads to microbial degradation or inactivation of the crystal proteins 2 . Most recently, several methods and studies are underway to develop different formulations for entomopathogens that can improve this activity and retain the microorganisms from solar degradation and other the harmful effects of the environment. Therefore, to overcome these limitations, such as susceptibility to light, short remnant lifetime and other conditions, optimization of Bt toxin formulation by nanoparticles is an essential parameter for its commercial production 5 . Ideally, the formulation should provide a maximum protective effect to the active agent, while it should have no antibacterial impact or the least antibacterial effect 6 .
Titanium dioxide (TiO 2 ) has become a whole part of nanotechnology because of its many abilities to act as a photocatalyst, UV absorber and assist environmentally useful reactions. TiO 2 occurs as three polymorphs, rutile, anatase, and brookite. However, because of the difficulty in its synthesis, brookite is less frequently introduced as photoactive material [7][8][9][10] . The bandgap of TiO 2 corresponds to about 3.0 eV for rutile and around 3.2 eV for anatase and it can just absorb ultraviolet light (UV) 11 . Despite the more significant band gap of anatase compared with rutile, it is observed that the photoactivity performance of anatase generally is considered more than rutile. This is attributed to factors that improved performance including a higher density of localised states and consequent surface-adsorbed hydroxyl radicals and lower charge carrier recombination in anatase relative to rutile 12 .
On the other hand, the photoactivity of the pure phased is smaller than the phase mixture of different polymorphs of TiO 2 . However, for a long time, it is generally agreed that anatase exhibits a higher photoactivity compared to rutile TiO 2 . These polymorphs show several photoactivity performances due to their different properties [13][14][15][16] .
According to previous researches, amorphous TiO 2 nanoparticles are not photoactive 17 . Rapid recombination of photogenerated electrons and holes before they can be involved in relevant reactions due to the existence of any defects in the amorphous phase leads to photochemical inactivity 17 . In this study, we develop a cost-effective and environmentally friendly approach method to use Bt for the biosynthesis of TiO 2 nanoparticles. The effect of different polymorphs of TiO 2 nanoparticles as a material to increase the persistence of Bt active agents against ultraviolet radiation was investigated.

Results
Structure and properties of TiO 2 . X-ray diffraction analysis was used for determining the crystalline phase of TiO 2 nanoparticles. As seen in Fig. 1a, the XRD data of TiO 2 (amorphous) indicated any discernible reflection pattern. Figure 116) and (215) and indicated the tetragonal structure of anatase TiO 2 nanoparticles. This confirms the occurrence of a transition from amorphous to crystalline anatase phase at 450 °C. There is a significant correlation between the peaks from the TiO 2 sample spectra and the library spectra of two different phases of TiO 2 . Figure 1c shows the XRD pattern of TiO 2 (rutile) powder (JCPDS:01-086-0147  Figure 1d shows the XRD pattern of TiO 2 (mix) (JCPDS:00-004-0477) which includes a mix of peaks related to both phases. It is proved that the crystallinity increased with increasing calcination and, also the anatase phase emerges 450 °C, while conversion to rutile appears between 800 °C to 1200 °C 18 .
XRD patterns show that the peaks of TiO 2 (anatase) steadily become sharper and narrower with calcination temperature until T = 1200 °C, at which transition toTiO 2 (rutile) is completed. Although, the transformation from anatase to rutile starts typically at much lower temperatures, around 600 °C. As the XRD analysis confirms, a mixture of phases is observed at 800 °C (Fig. 1c).
Considering the correction in FWHM for the line broadening of the instruments, the average particle size (D) was computed using the Scherrer equation (Eq.1): where λ is the X-ray wavelength of radiation, β is FWHM in radians and θ is the Bragg's angle. The crystallite size of the TiO 2 (anatase) and TiO 2 (rutile) is 15.7 and 79.24 nm, respectively. Figure 1e shows a PDF card of the crystalline phase of TiO 2 nanoparticles. The FTIR spectra of B. thuringiensis synthesized TiO 2 nanoparticles which according to XRD results in an amorphous structure, exhibited peaks at 3423, 2925, 1625, 1438, 1032, 500 cm −1 (Fig. 2a). A broad peak at 3430 cm −1 shows O-H stretching due to the phenolic and alcoholic group. The band at 2925 cm −1 in nanoparticles is corresponding to the symmetric stretch (C-H) of CH 3 and CH 2 groups of aliphatic chains. The peak at 1625 cm −1 indicates the presence of C=C ring stretching. The band observed at 1438 cm -1 is because of bending vibration of the CH 2 in the lipids and proteins. The peak in the range of 1257-1020 cm −1 corresponds to the C-O stretching of alcoholic and carboxylic groups. The band at 1032 cm −1 assigned to the C-N stretching vibrations of aliphatic amines. In TiO 2 (anatase) structure broadband observed in the range of 3600-3200 cm −1 is assigned to stretching hydroxyl group (O-H), representing the presence of surface water as moisture. The broad bands at 3445 and 1622 cm −1 observed in TiO 2 (anatase) structure spectra are related to the Ti-OH stretching modes (Fig. 2b). In the TiO 2 (rutile) structure the Ti-OH vibration band becomes much weaker and the full removal of the absorption peak at 1622 cm −1 infer the absence of hydroxyl groups in TiO 2 (rutile) structure (Fig. 2c) and, as expected, in TiO 2 (mix) structure, the peaks of both anatase and rutile were seen (Fig. 2d). Figure 3 shows the morphology of the TiO 2 nanoparticles at different temperatures. The SEM images of the synthesized TiO 2 nanoparticles by B. thuringiensis show spherical clusters of the nanoparticles. Figure 3a is an image of the amorphous TiO 2 powder. As seen, there is no definite morphology, and just disordered agglomerates with different sizes appeared. Figure 3b presents the images of TiO 2 nanoparticles calcined at 450 °C, which demonstrate the small size of the particle. TiO 2 nanoparticles were irregular spherical structure, oval in shape, spherical, and a few aggregates having a size of 33-44 nm. The width of the anatase peak diffraction from XRD confirms the smaller crystalline size at 450 °C. By increasing temperature to 1200 °C, the size increases, and the grain growth becomes remarkable (Fig. 3c), which describes the effects of heat treatment on the particle size of TiO 2 nanoparticles. Figure 3d shows the images of TiO 2 nanoparticles calcined at 800 °C. As you can see, it contains two different phases of TiO 2 nanoparticles.
Energy-dispersive X-ray spectroscopy (EDX) analysis of TiO 2 nanoparticle (anatase and rutile) illustrates peaks for Ti element and oxygen. As shown in Fig. 4, there is no trace of any other impurities in the EDX analysis.
www.nature.com/scientificreports www.nature.com/scientificreports/ Effect of UV radiations on spore viability. The effects of different polymorphs of TiO 2 on the viability of B. thuringiensis formulations following exposure to UVA (385 nm) irradiation for five days are summarised in Fig. 5. After 72 h, the spore viability decreased from their initial values (100%) and reached to 79.76, 70.36, 56.63, 42.20, and 41.32% for TiO 2 (mix), TiO 2 (anatase), TiO 2 (rutile), TiO 2 (amorphous) and non-protected formulation, respectively. According to the results, there is a significant difference between the crystalline phase of TiO 2 and the free spore of Bt, but the difference between TiO 2 (amorphous) and the free spore of Bt is not significant. TiO 2 (rutile) displays less protection of spore viability than TiO 2 (anatase) and TiO 2 (mix) sample shows the maximum protection of spore viability. Our results suggest that a mixture of anatase and rutile formulation could be a proper perspective for improving persistence and subsequently modifying the performance of Bt against UVA. This can be attributed to the photoactivity of each polymorph. The photoactivity of TiO 2 is forcefully dependent on its crystallite size, phase structure, specific surface areas, and pore structure 19 . Photoactivity improved with increasing calcination temperature from 450 °C to 800 °C, which is when converting anatase to rutile. Larger grain size, lower specific surface areas and having a worse surface adsorption content of rutile causes lower photoactivity than anatase. As the results indicate, TiO 2 (rutile) formulation than less protection of spore viability than TiO 2 (anatase) formulation. Whereas anatase shows a higher photoactivity than rutile and amorphous phase with a higher level of adsorbed radicals that because of a more top surface area as well as a higher photoactivity per unit of surface area and as it was observed the protective effect of TiO 2 (anatase) formulation is greater than that of TiO 2 (rutile) and TiO 2 (amorphous) formulations.
A combination of two polymorphs of TiO 2 improves the photoactivity because of the more effective spatial separation of the photoinduced electron-hole pair which been reported to display enhanced photoactivity relative to single-phase TiO 2 . It is worth mentioning that this is an outcome of better charge carrier separation, maybe through the trapping of electrons in TiO 2 (rutile) and the effective reduction in the rate of recombination of charge carrier 20,21 .
According to our previous research results, among all examined formulation, GO/olive oil formulation has the highest protection of spore viability 5 . The continuation of formulation's exposure to UV radiations up to 72 h showed that spore viability for GO/olive oil formulation declined to 69.9%. While spore viability in two www.nature.com/scientificreports www.nature.com/scientificreports/ formulations of TiO2(mix) and TiO2(anatase) is more than the same parameter for GO/olive oil formulation. Thus, the sensitivity of Bt active agent to UV radiations limits its persistence in field conditions and justifies the addition of UV protectants in formulations for improving the stability and efficacy in the environment Table 1, the larval mortality of irradiated TiO 2 (mix), TiO 2 (anatase), TiO 2 (rutile), TiO 2 (amorphous) and non-protected formulation, after 72 h exposure to UVA radiation were 73.76%, 71.24%, 57.12%, 51.32%, and 50.32%, respectively (Fig. 6). The results proved that there was a significant difference between the four treatments (Duncan test, P < 0.05), whereas there was no significant difference between  www.nature.com/scientificreports www.nature.com/scientificreports/ TiO 2 (amorphous) formulation with non-protect Bt formulation at p = 0.05. On the other hand that no protection was observed in the case of TiO 2 (amorphous) formulation. Among the four formulations, TiO 2 (mix) showed the maximum performance in mortality of larvae which is confirmed by difference in photoactivity of different polymorphs of TiO 2 . According to the results found by Poszgay et al., after 40 h of exposure to UV activity of Bt well is lost 22 . However, the spores and crystals of TiO 2 formulation were able to remain active after 72 hours.   www.nature.com/scientificreports www.nature.com/scientificreports/

Discussion
Bt is applied to kill many different insects and has been widely for pest control, and a commercial formulation of this has been used as an alternative to chemicals. Sunlight as one of the most important natural environmental stress is known to inactivate the biopesticide preparations based on Bt because of UV radiation. According to library studies, we have applied a hitherto unreported, inexpensive, modern material, non-toxic for the quick synthesis of TiO 2 nanoparticles. The protective effects of different polymorphs of TiO 2 after exposure to UVA radiation were verified by the spore viability and crystal activity. Moderate protection has been shown by using TiO 2 (anatase) and TiO 2 (rutile), while TiO 2 (mix) formulation of Bt improved the resistance of the spores to UV irradiation. Therefore, synthesized TiO 2 (Mix) nanoparticles has the best performance in resisting Bt against UVA.

Methods
Materials. Titanium(IV) ethoxide (Ti(OC 2 H 5 ) 4 ) were purchased from Sigma-Aldrich and used without any purification prior to preparing TiO(OH) 2 . nutrient agar was purchased from Merck Chem. Co. (Germany). Deionized (DI) Water applied throughout all experiments and was purified with the Millipore system. B. thuringiensis subsp. Kurstaki KD-2 was obtained from Green Biotech (Tehran, Iran).
Biosynthesis of tio 2 nanoparticles using Bt. TiO(OH) 2 was used as a precursor for the biosynthesis of TiO 2 , where it was synthesized according to the previous references 23 . In summary, it was prepared by adding Ti(OC 2 H 5 ) 4 to water as a molar ratio of 1: 26.3. The mixture was stirred for 6 h at 25 °C. Afterwards, the resulting precipitate was filtered and washed several times with deionized water and, ethanol then dried in an oven at 90 °C overnight 24 .
For the biosynthesis of TiO 2 nanoparticles, mother culture is provided after growing the Bt cells as a suspension culture in sterile distilled water containing suitable carbon and nitrogen source for 48 h, and this was treated as a mother culture. Then, 50 mL of this solution was diluted by adding 150 mL of sterile distilled water containing nutrients. Next, this solution was again allowed to grow for another 24 h. After adding 40 mL of 0.025 M TiO(OH) 2 solution to the culture solution, it was heated for 30 min on steam bath up to 60 °C until white precipitation emerges at the bottom of the flask. The culture solution was incubated at 21 °C in the laboratory condition. After 48 h, the culture solution was observed to have separately white clusters deposited at the bottom of the flask 25 .
Further, the precipitate was filtered, washed with deionized water and, dried at 90 °C. After calcination, the amorphous phase of TiO 2 was transformed into the crystalline phase. Different fractions of the obtained powder were calcinated at different temperatures 450 °C, 800 °C, and 1200 °C for 3 h, for the synthesis of TiO 2 (anatase), TiO 2 (mix), TiO 2 (rutile), respectively.
Characterization of synthesized TiO 2 nanoparticles. The X-ray spectra were recorded on a Phillips X'Pert PRO using filtered Cu Kα radiation (λ = 1.54178 Å) over the range 10° < 2θ < 80°. Fourier transform infrared (FT-IR) spectra (4000-400 cm −1 ) by a Tensor 27 spectrometer (Bruker, Saarbrucken, Germany) were also used to confirm the TiO2 phase. SEM analysis of nanoparticles and nanocomposite were performed with a scanning electron microscope (FE-SEM, Sigma, Zeiss) equipped with energy dispersive X-Ray (EDX) elemental composition analyzer.
Preparation of nano-formulation. After dispersing 0.01 g of different polymorphs of TiO 2 in 100 mL sterile distilled water with ultrasonic vibration for 1 h, the mother culture of Bt cell (prepared in the previous section) was added and placed on a shaker set in a dark place for 24 h 5 .
Evaluating of spores viability. Radiation was supplied by UVA tubes (Philips, 15 W, white light 385 nm peak emission) mounted 180 mm above the open Petri dishes. 40 mL of each formulation of different polymorphs of TiO 2 were put on Petri dishes in triplicate. They were then exposed to UVA irradiation for 24, 48, 72, 96 and 120 h. Spore count of these samples was carried out by serial dilution with nutrient agar medium (CFU). Via serial dilution of formulations spread in Petri dishes containing nutrient agar spore counts was carried out and incubated at 28 °C for 24 h. The percentage of spore viability was estimated according to Eq. 2: x 0 where N x is a number of the irradiated spores (free formulation or nano), and N 0 is the number of the initial non-irradiated spores (free spores or nano) 2 .
Bioassay. The toxicity of the four different formulations of TiO 2 (irradiated and non-irradiated) and free spore (irradiated and non-irradiated) on second-instar larvae of Ephestia kuehniella Zeller was investigated. These larvae were reared on a diet containing a mixture of wheat bran, wheat flour under a long day (8 h dark, and 16 h light) at 28 °C with 60% relative humidity. After separating the second-instar larvae from flour medium, for the bioassay test six peanut pieces were soaked in 35 mL of the different formulations under sterile conditions for 4 min as larval food and then dried and placed in Petri dishes with 15 larvae. Next, the Petri dishes were incubated at 27 °C and 60% humidity. Mortality was recorded every 24 h and compared to the control for 10 days. Each treatment was carried out in triplicate 26 .
Statistical analysis. All tests were performed using the Statistical Package for the Social Sciences software (SPSS 1998). All of the treatments were the average of three replicates of separate runs and compared using the Duncan test after analysis of variance (ANOVA).