Sequences and SEC profiles of MBP constructs used. (A) Annotated sequence alignment was prepared using Espript 3.0 (https://espript.ibcp.fr). Passenger sequences are coloured according to hydrophobic residues: red; polar residues: green; basic residues: pink; acidic residues: blue. (B) SEC profile (Pharmacia S200) following large-scale purification. (C) Analytical SEC profile (S200 10/300). Figures were prepared using GraphPad Prism 6.0 (https://graphpad.com).