Table 1 Correlation of CYP isoform protein content with CYP marker activities measured in dog liver microsomes.

From: Pharmacogenomics of poor drug metabolism in Greyhounds: Cytochrome P450 (CYP) 2B11 genetic variation, breed distribution, and functional characterization

Enzyme marker activityAttributed CYPsSpearman correlation coefficient (P-value)
HumanDogCYP1A proteinCYP2B11 proteinCYP3A protein
Phenacetin-o-deethylationCYP1A2CYP1A20.59 (0.02*)0.30 (0.28)0.10 (0.71)
Coumarin 7-hydroxylationCYP2A6CYP2A13−0.29 (0.29)−0.35 (0.20)0.06 (0.83)
Propofol 4-hydroxylationCYP2B6CYP2B110.32 (0.24)0.70 (0.003*)0.18 (0.51)
Bupropion 6-hydroxylationCYP2B6CYP2B110.25 (0.36)0.73 (0.002*)0.00 (1.00)
Flurbiprofen hydroxylationCYP2C9?0.41 (0.13)0.42 (0.12)0.14 (0.61)
S-mephenytoin 4-hydroxylationCYP2C19?0.38 (0.16)0.28 (0.31)0.15 (0.56)
Dextromethorphan o-demethylationCYP2D6CYP2D15−0.31 (0.26)−0.47 (0.07)−0.05 (0.84)
Chlorzoxazone 6-hydroxylationCYP2E1CYP2E10.06 (0.81)−0.10 (0.71)−0.04 (0.87)
Omeprazole sulfonationCYP3A4CYP3A120.13 (0.64)0.35 (0.20)0.86 (<0.001*)
  1. CYP isoform protein content (determined by immunoblotting) and CYP marker activities were measured in the same set of dog liver microsomes (n = 15) and correlated. Shown are the Spearman correlation coefficients and associated P-values (*P < 0.05). Also shown are the human and canine CYP isoforms that have been attributed to each activity by reaction phenotyping. (?) – No evidence yet to identify the dog CYP isoform responsible for this activity.