Reaction phenotyping using a panel of recombinant canine CYP enzymes. The rates of propofol 4-hydroxylation (a,b) bupropion 6-hydroxylation (c,d) and omeprazole sulfonation (e,f) were determined using a panel of 11 recombinant canine CYP enzymes. Results are shown after normalization to incubation time and recombinant CYP concentration in each reaction (a,c,e) as well as after extrapolation of activities to microsomes using the reported average molar concentration of each CYP in canine liver microsomes (b,d,f). Details are provided in Materials and Methods section. Activities for pooled dog liver microsomes (pDLMs) normalized to microsomal protein content are also shown for comparison. Bars represent the mean and standard deviation of 3 independent replicate experiments.