Abstract
Pectins, the major components of cell walls in plants, are synthesized and secreted to cell walls as highly methyl-esterified polymers and then demethyl-esterified by pectin methylesterases (PMEs). The PMEs are spatially regulated by pectin methylesterase inhibitors (PMEIs). In this study, 43 and 49 putative PME and PMEI genes were identified in maize, respectively. Gene structure and motif analysis revealed that members in the same paralogous pairs or in the same subgroup generally had common motif compositions and gene structure patterns, which indicates functional similarity between the closely related ZmPME/PMEI genes. Gene ontology annotation analysis showed that most of the ZmPME/PMEI genes are involved in cell wall modification and pectin catabolic process with molecular functions of pectinesterase or pectinesterase inhibitor activities. There are 35 ZmPME/PMEI genes expressed higher in anthers than in other tissues from the NimbleGen maize microarray data, and the semiq-RT-PCR assay revealed most of these ZmPME/PMEIs specially expressed in anthers and pollens, indicating they possibly had role in anther and pollen development. In addition, these ZmPME/PMEI genes were highly expressed in the fertile anthers, while lowly or no expressed in sterile anthers. This further indicated these genes might be involved in the development of anther and pollen.
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Introduction
Pectins, which are synthesized from nucleotide sugars, are the major components of cell walls in plants1,2,3,4. PME and PMEI play a central role in the synthesis and metabolism of pectins5,6. There are 66 PMEs in Arabidopsis7, 16 in Phytophthora sojae8, 35 in rice9, 105 in flax10, and 81 in G. raimondii11. For the PMEIs, 71, 49, 95 and 100 PMEIs were identified in the whole genomes of Arabidopsis12, rice13, flax10 and B. campestris14, respectively.
PMEs (EC. 3.1.1.11) are enzymes belonging to the class 8 of the carbohydrate esterases15 (CAZY: http://www.afmb.cnrs-mrs.fr/CAZY/). The mature and active region of PME genes mainly consists of the PME domain. In higher plants, the PME genes are classified into two types, type I and type II. They share a catalytic PME domain at the C-terminus, and proteins in type I also have a domain (PRO-region) at the N-terminal region sharing similarities with the PMEI domain, which demonstrated roles on early demethylesterification of pectins in the Golgi apparatus16,17,18. The PMEs catalyze the demethylesterification of homogalacturonan component of pectin, which generates carboxyl groups during the release of methanol and hydrogen ions19. This enzymatic activity of the PMEs can lead either to cell wall loosening or to cell wall stiffening, depending on the apoplastic pH6,19,20, which is sometimes associated with growth21, and cell-to-cell cohesion22. Pectin demethylesterification is catalyzed by a number of the PMEs isoenzymes which can express their activities in response to certain developmental or environmental cues and/or in a tissue-specific fashion. For example, while some PMEs are ubiquitously present23, others are specifically expressed during root development22, fruit ripening24,25, or stem elongation26,27. Analysis of pollen-specific transcriptome of Arabidopsis indicated that several PMEs are specifically expressed in floral buds28. Furthermore, in Arabidopsis some PME genes (At5g49180, At1g11590 and At4g02300) might be involved in the early event of embryo/seed development7. The PMEIs, first identified in kiwi5,29, typically inhibit the PMEs of plant origin by covering the shallow cleft of the PMEs and forming a reversible stoichiometric 1:1 protein complex30. Post-translational regulation of the PMEs via PMEIs represents another important control mechanism29. For example, OsPMEI28 overexpression in rice had an effect on the growth process, which resulted in a dwarfed phenotype31, and overexpression of the PMEI5 resulted in a higher demethylesterification of seeds and reduced the PME activity, which was accompanied by an earlier and faster germination process compared to wildtype in Arabidopsis32.
In recent years, many reports have shown that some PME/PMEIs regulate plant stress resistance and pollen development. AtPMEI10, AtPMEI11 and AtPMEI12 were identified as upregulated in response to B. cinerea infection33. Expression profile of the genes TaPME21-2, TaPME21-1/2/4, TaPME58, TaPME63 and TaPME67 was induced in the susceptible cv. Bobwhite and repressed in the resistant cv. Sumai 334. The transgenic rice overexpressing OsPME14 showed higher PME activity and Al content in root tip cell wall, and became more sensitive to Al stress9. In flax, 48 (77.4%) PME genes and 53 (80.3%) PMEI genes had higher expression level in the flowers10. In Arabidopsis, 15 PMEs were highly expressed in pollen and 10 of these contained PRO regions35. These suggest that the PME/PMEIs might play important roles in pollen development. Mutations of VANGUARD1 (VGD1), the type I PME gene with the highest expression levels in Arabidopsis pollen tubes, resulted in retarded growth in the style and transmitting tract and subsequent reduction in male fertility36. In maize, the ZmC5 of PMEs has a role in pollen tube elongation37 and ZmGa1P, a pollen-expressed PME gene, can confer the male function in the maize unilateral cross-incompatibility (UCI) system38.
In this study, genome-wide identification of ZmPME/PMEI genes was firstly conducted in maize, and the phylogenetic tree, gene structure, conservative motif, expression, gene ontology annotations were also examined. In addition, semiq-RT-PCR assay was conducted to verify the gene expression pattern of some ZmPME/PMEI genes highly expressed in anthers, since more than half the genes highly expressed in anthers according to the NimbleGen maize microarray data. To further evaluate their possible roles on pollen development, gene expression of some ZmPME/PMEI genes in fertile and sterile anthers was also investigated. Results in this study would provide useful information for further investigate the function of maize PME/PMEIs, especially on the development of anther and pollen.
Results
Identification of ZmPME/PMEI genes in maize
To identify putative ZmPME/PMEI genes in maize genome, we searched the maize genome annotation data with known plant PME/PMEI domains (pfam01095/pfam04043) as a query using HMMER 3.0 package39. In total, we obtained 43 putative PME genes and 49 putative PMEI genes in maize. These genes were designated as ZmPME1-43 and ZmPMEI1-49 (Fig. 1 and Supplementary Table 1), of them, 20 genes (PME1-20) had PRO region (which showed similarities with the PMEI domain) and the PME domain. Each ZmPME/PMEI gene model was selected by analyzing the similarity between the ZmPME/PMEI genes and homologous genes, as most of the ZmPME/PMEI genes had more than one transcript in the MaizeGDB database (https://www.maizegdb.org/). Then we randomly selected 15 ZmPME/PMEIs for reverse transcription polymerase chain reaction (RT-PCR) to assess the veracity of the ZmPME/PMEI genes models. The results indicated that the 15 ZmPME/PMEI genes were expressed in maize pollen and only a single amplicon was found (Supplementary Fig. S1). The most identified ZmPME/PMEI genes encode proteins with 150-250 amino acids (aa). They are ranging from 149 (ZmPME23) to 1,360 (ZmPME28) aa, with an average of 346 aa (Supplementary Fig. S4), and their isoelectric points (pI) are 4.28 to 10.23. These ZmPME/PMEIs are distributed on all the 10 maize chromosomes, and chromosomes 1, 2, 3, 7 and 8 have more ZmPME/PMEIs than others (Supplementary Fig. S2).
Phylogenetic analysis
Phylogenetic trees were constructed by using MEGA 7.0 with the neighbor-joining model. In order to analyze the evolutionary relationships among the predicted ZmPMEs and ZmPMEIs, we aligned maize acid sequences with 101 and 106 predicted PMEs and PMEIs from rice and Arabidopsis. On the basis of phylogeny, the PMEs and PMEIs families in plants were subdivided into 5 and 12 groups, respectively (Supplementary Fig. S3). PMEs in each group and PMEIs in groups I to III, V, VI and VIII are all from the three species (Supplementary Fig. S3), indicating that these ZmPME/PMEIs might have the conserved function in evolution.
Meanwhile, according to cluster analysis, the ZmPME/PMEI families could be divided into 5 and 8 subfamilies, respectively (Fig. 1). The PMEI domains may be derived from duplication and divergence of the PRO domain and have rapidly evolved12. So, we constructed the PMEI phylogenetic tree used the protein sequences of all the ZmPMEIs and the 20 ZmPMEs containing PRO region. The ZmPMEI subfamily I includes the same genes in the ZmPME subfamily I (except ZmPME21 and ZmPME22), both of the subfamilies are the largest subfamily, and the genes had signal prediction or transmembrane region domain. For ZmPMEs, genes in the subfamilies II and III do not have signal prediction or transmembrane region domain (except ZmPME27); while genes in the subfamily IV have signal prediction domain (except ZmPME32 and -35); and the pI of subfamily V are higher than 8. For ZmPMEIs, genes in the subfamilies II and VII have signal prediction domain, and their pI are higher than 7 (except ZmPMEI26, -37 and -41); most genes in the subfamilies III, IV and VIII expressed higher in anthers than in other tissues (Fig. 2). Homologous ZmPME/PMEI genes were identified 24 paralogous pairs in maize (Supplementary Table 2). The value of the nonsynonymous substitution rate (Ka) to the synonymous substitution rate (Ks) substitutions (Ka/Ks) can be used as an indicator which could reflect selection pressure of a gene or a gene region during evolution. To infer the influence of selection on the evolution of the maize, we estimated Ka/Ks values for all of them (Supplementary Table 2). The Ka/Ks values of all the homologous genes are between 0.0033 and 0.3889, suggesting that most of the ZmPME/PMEI genes undergone negative selection and evolved slowly. The Ka/Ks values of maize PMEs paralogs are significantly lower than that of the PMEIs homologs (P < 0.005).
Gene structure and motif analysis of the ZmPME/PMEI families
Gene structures of the ZmPME/PMEI genes were constructed by aligning the extracted genomic sequences to predicted cDNA sequences of the maize PME/PMEI genes. As can be seen from Supplementary Fig. S5, most of the ZmPME genes in subfamily I have 2 exons, and the ZmPME members in the subfamily III have 5 introns (except ZmPME28). In addition, most of the ZmPME genes contain 1–10 introns, and most of the ZmPMEI genes do not have no intron.
Analysis of the ZmPME/PMEI protein sequences with MEME (http://meme-suite.org/tools/meme) revealed 6 conserved motifs of the ZmPME genes, and 9 conserved motifs of the ZmPMEI genes (Supplementary Table 3). Of the ZmPMEs, 36 proteins contain motifs 1, 2, 4, 5 and 6 (except ZmPME21, -23, -24, -25, -27, -29, -32, -34 and -43, Supplementary Fig. S6). For the ZmPMEIs, the proteins in the subfamily I (containing both PME and PMEI domains) have motifs 1 to 9 (except ZmPM13 to ZmPME16), and the rest of ZmPMEIs contain motifs 8 and 9 (except a few ZmPMEI genes, Supplementary Fig. S7).
As expected, most closely related members have a common motif composition and gene structure pattern, which indicates functional similarity between the ZmPME/PMEI proteins in paralogous pairs or in the same subfamily (Fig. 3). For the ZmPME genes, proteins in the subfamilies IV and V contain motifs 1–5 (except ZmPME32, -34 and -43) and the intron phase 2, 0, 0 and 2 separating the PME domain (except ZmPME32, Fig. 3a,b). Proteins in the subfamily VII of the ZmPMEIs have motifs 8 and 9 (except ZmPMEI37 and -41), while that in the subfamilies II, III and IV have motifs 7, 8 and 9 (except ZmPMEI35 and -40, Fig. 3c,d), and most of ZmPME genes in the subfamily IV have 5 exons.
The PME and PMEI domains of the ZmPME/PMEIs, the PME domains in E. chrysanthemi (GenBank: Y00549), carrot (SwissProt Accession No. P83218) and A. aculeatus (Swiss-Prot code Q12535); and the PMEI domains in kiwi (SwissProt Accession number No. P83326) and Arabidopsis (AthPMEI-1, Accession Number NP_175236; AthPMEI-2, Accession Number NP_188348) were analyzed by T-Coffee (http://tcoffee.org/503/index.html) and displayed by ESPript 3.0 (http://espript.ibcp.fr/ESPript/cgi-bin/ESPript.cgi, Supplementary Figs. S9 and S10). The ZmPMEs contain five characteristic sequence fragments (44_GxYxE, 113_QAVAL, 135_QDTL, 157_DFIFG, 223_LGRPW; carrot numbering), and several highly conserved aromatic residues (Supplementary Fig. S9). The ZmPMEIs contain four conservative Cys residues, which were connected by two disulfide bridges (first to second and third to fourth) and do not have the fifth conservative Cys residue which has a free thiol group comparing to kiwi and Arabidopsis.
To further understand the structure of the ZmPME/PMEI proteins, three-dimensional (3D) structure of the PME/PMEI domains in ZmPME3 and ZmPMEI2 were analyzed by I-TASSER (https://zhanglab.ccmb.med.umich.edu/I-TASSER/), and exhibited by Chimera1.8.1 (http://www.cgl.ucsf.edu/chimera/, Fig. 4). ZmPME3 has high similarity with the PME (PDB 1GQ8) from Carrot40 (C-score = 1.71, TM-score = 0.95 ± 0.05, RMSD = 2.8 ± 2.0, Fig. 4b). Furthermore, ZmPMEI2 has high similarity with the PMEI (PDB 1xg2B) from kiwi30 (C-score = 1.7, TM-score = 0.86 ± 0.07, RMSD = 2.7 ± 2.0, Fig. 4d). Superposition of the known PME structures of carrot and maize (ZmPME3, Fig. 4b), and PMEI structures of kiwi and maize (ZmPMEI2, Fig. 4d) confirm the similarity of the folding topologies.
Gene ontology (GO) annotation and subcellular localization of the ZmPME/PMEI proteins
The 92 ZmPME/PMEI genes (except ZmPME28) were assigned a total of 37 GO terms (Fig. 5 and Table 1). Among them, 175, 78 and 167 proteins were assigned terms under molecular function, cellular component and biological process, respectively. Under biological process, 41 ZmPME genes were predicted to be involved in cell wall modification, 42 ZmPME genes were related to pectin catabolic process and 73 genes (all of ZmPMEI genes and ZmPME21, -22, -23 and -24) were involved in negative regulation of catalytic activity. Under cellular component, 41 ZmPME genes were assigned to cell part. Under molecular function, most of the ZmPME genes and a few ZmPMEI genes had pectinesterase activity and aspartyl esterase activity; and most ZmPMEI genes and a few ZmPME genes had pectinesterase inhibitor activity or enzyme inhibitor activity. In addition, we analyzed the GO annotations for each subfamily. The same annotations exist in different genes of different subfamilies (e.g., the PMEI genes in the subfamilies II and III), and there are also different annotations for genes in the same subfamily (e.g., the PME genes in the subfamily II, Supplementary Table 4). These results suggested that different genes in the same subfamily may have different roles in the evolution process.
Subcellular localization of the 92 ZmPME/PMEIs were predicted using TargetP (http://www.cbs.dtu.dk/services/TargetP/) and WoLF PSORT (https://wolfpsort.hgc.jp/). Majority of the proteins (77, 83.7%) were revealed as signal peptides by TargetP; five (5.4%) are located in mitochondria; and ten are not assigned (Supplementary Table 1). Moreover, the WoLF PSORT predicted a number of ZmPME/PMEIs (93.5%) locating to chloroplast or extracellular (Supplementary Table 1). In addition, a ZmPMEI gene (ZmPMEI16) was found to be targeted to chloroplast by an in vivo transient expression assay (Supplementary Fig. S8). This consistent with the prediction of WoLF PSORT.
Expression assay of the ZmPME/PMEI genes
The NimbleGen maize microarray data41 (ZM37) including 60 tissues representing 11 major organ systems and various developmental stages of the B73 maize inbred line was employed to analyze the expression pattern of the ZmPME/PMEI genes. All of the ZmPME/PMEI genes (except one ZmPME gene and 13 ZmPMEI genes) expression data was used to draw Heatmap. Of them, 35 ZmPME/PMEI genes had a much higher expression level in anthers than in other tissues (Fig. 2), these ZmPME/PMEI genes may be related to the development of anther or pollen. In general, expression pattern was similar for genes within the same paralogous gene pairs (e.g., ZmPMEI1/-17, Supplementary Table 1 and Fig. 2), indicating they might be formed by segmental duplication and retained their function. However, the expression profiles of the four paralogous gene pairs (ZmPME12/-13, ZmPME14/-15, ZmPME16/-17 and ZmPMEI29/-38) were fundamentally different in different tissues, suggesting that these genes may have differentiated with different roles.
To confirm the organ-specific expression of ZmPME/PMEI genes shown by the microarray data, 14 ZmPME/PMEI genes specifically expressed in anthers, ZmPME24 (not including in the maize microarray data) and ZmPME30 (expressed low in all tissues) were selected for conducting semiq-RT-PCR. Semiq-RT-PCR was performed with total RNA isolated from the roots, leaves, ears, immature tassels, pollens, anthers, whole seed (after pollinated), endosperm and embryo of the B73 inbred line. Fourteen of them, ZmPME3, -5, -7, -11, -23, -31, -42, -43 and ZmPMEI2, -16, -25, -31, -32, -44 matched well with the microarray data, all of these ZmPME/PMEI genes expressed significantly higher in the anthers or pollens than in other organs. It is interesting to note that ZmPME23 was specifically expressed in the anthers. The gene not included in the microarray data, ZmPME24, was also specifically expressed in the anthers and pollens. However, expression of only one gene, ZmPME30, did not match with the microarray data, it had higher expression level in the anthers and pollens but showed little or no expression in roots, leaves, ears and seed according to the semiq-RT-PCR assay (Fig. 6a).
To further analyze the possible role of the ZmPME/PMEI genes on anther or pollen development, expression of the 13 genes (which had identified specifically expression in the anthers or pollens of B73 inbred line) was investigated in the anthers of three fertile and three sterile individuals of a maize backcrossing population derived from a cytoplasmic male sterility (CMS) line. The results showed that all of the selected ZmPME/PMEI genes were differentially expressed in the fertile and sterile anthers, although some ZmPMEI genes showed lower expression in the sterile anthers (Fig. 6b).
Discussion
Genome-wide analysis has identified the PMEs and PMEIs in nearly all vascular plants and in multiple gene members18. Up to now, the function of a number of the PME genes have been studied in Arabidopsis42, rice9, pea43, wheat44, and cotton45; and the PMEI genes in Arabidopsis46,47, rice13, broccoli48, and Chinese cabbage49. Most of them involved in plant growth and various stress responses (reviewed by Wormit and Usadel50). In maize, however, only three ZmPMEs (ZmC5, ZmPme3 and ZmGa1P) and one ZmPMEIs (ZmPMEI1) were characterized and found to be involved in pollen tube elongation37,38,51,52. Thus genome-wide identification, evolution, and expression analysis of the PME/PMEI families in maize will facilitate to understanding of the function of the gene families.
In this study, 43 and 49 ZmPMEs and ZmPMEIs were identified in the maize genome, which were divided into 5 and 8 subfamilies (Supplementary Table 1; Fig. 1), respectively. The number of ZmPMEs is less than that identified in Arabidopsis7, and Gossypium raimondii11, while that of ZmPMEIs is much more than that identified in Sorghum bicolor12, and Brachypodium distachyon53. We identified 24 paralogous pairs in maize, but all Ka/Ks values of paralogous are less than 1 (Supplementary Table 2), implying that ZmPME/PMEI genes evolved mainly under the influence of stabilizing selection. The result of Ka/Ks analysis of PME, PRO and PMEI domains reveals that the homologous gene pairs of Arabidopsis, rice and sorghum experienced purifying selection12 and the PME homologous gene pairs of G. arboreum, G. raimondii and G. hirsutum also experienced stabilizing selection45. Thus ZmPME/PMEI genes might play important role in growth and development of plants.
Intragroup ZmPME/PMEIs have conserved gene structure and motif composition, indicating that ZmPME/PMEIs in the same group could have the same function and they might come from a common ancestor. For instance, the ZmPME subfamilies IV and V contain motifs 1–5 (except ZmPME32, -34 and -43) and most intron phases 2, 0, 0 and 2 separating the PME domain (Fig. 3a,b). Of the ZmPMEIs, most members in the subfamilies III, IV and VIII expressed higher in anthers than in other tissues (Fig. 2), and most members in the subfamily VII have motifs 8 and 9 (Fig. 3c,d). The PME and PMEI domains alignment implying that the ZmPME domains have five characteristic sequence fragments (44_GxYxE, 113_QAVAL, 135_QDTL, 157_DFIFG, 223_LGRPW; carrot numbering, Supplementary Fig. S9), which have all been shown to be functionally important in carrot54; the ZmPMEI domains have four conservative Cys residues (Supplementary Fig. S10), which are connected by two disulfide bridges, but do not have the fifth conservative Cys residue in comparison with that in kiwi29 and Arabidopsis55. The structure of the carrot PME is almost completely superimposable to the structure of tomato30. In this study, the 3D structure of ZmPME3 and ZmPMEI2 are highly similar to that from Carrot40 and kiwi29, respectively. This indicated that the PME and PMEI domains were highly conserved in different plant species.
In recent years, there are many reports on the function of PME/PMEI genes. Overexpression of PMEI5 in Arabidopsis thaliana caused aberrant growth morphology of the stems56; VvPMEI1 expression negatively correlates with the PME activity during the early stage of grape berry development of Grapevine57. The expression pattern of the ZmPME/PMEI genes from the NimbleGen maize microarray data showed 35 ZmPME/PMEI genes had a much higher expression level in the anthers than in other tissues (Fig. 2), and semiq-RT-PCR analysis of different tissues from B73 inbred line verified that they had much higher expression in the anthers or pollens (Fig. 6a). In addition, semiq-RT-PCR analysis of some ZmPME/PMEI genes showed that 13 ZmPME/PMEI genes were differentially expressed in the anthers of fertile and sterile individuals derived from a maize S-type CMS line (Fig. 6b). Similar results also have been reported in other plant species. For example, antisense expression of a pollen-specific PMEI from broccoli (Brassica oleracea) in Arabidopsis triggered silencing of the orthologous Arabidopsis gene At1g10770 and resulted in male sterility48; the expression of the pectin methylesterase gene (At3g06830) was significantly lower in male-sterile line than in male-fertile line at the <1 mm anther length stage of Brassica napus58; in cotton, transcriptome analysis showed that many pectin methylesterase genes highly expressed in flowering buds of fertile plants compared to those of the CMS-D8 line59. This implied that a number of ZmPME/PMEI genes might play an important role in anther and pollen development, however, their detailed roles in male function of maize need to be further studied in future.
Materials and Methods
Identification of the PME/PMEI genes in maize
Maize genome sequences were downloaded from the Maize Genome Database (Maize GDB; https://www.maizegdb.org/). Local HMMER3.039 (E-value-10) searches were performed using the Hidden Markov Model (HMM) profile in the Pfam database (http://pfam.janelia.org/search/sequence) to screen all candidate ZmPME/PMEI gene sequences. Candidate genes were retained that contained known conserved domains and passed checks against the Pfam (http://pfam.janelia.org/) and SMART (http://smart.embl-heidelberg.de/) databases for presence of the PME/PMEI domains (PF01095/PF04043). Bioinformatics analyses were performed on the ZmPME/PMEI protein sequences, and physical and chemical parameters (e.g., MW, pI) were calculated using ExPASy (http://www.expasy.ch/tools/pi_tool.html). TargetP (http://www.cbs.dtu.dk/services/TargetP/) and WoLF PSORT (https://wolfpsort.hgc.jp/) were used to predict the subcellular of ZmPME/PMEIs.
Analysis of gene structures and conserved motif of the ZmPME/PMEI genes
Several ZmPME/PMEI genes had more than one gene model annotated in MaizeGDB (https://www.maizegdb.org/). To confirm the putative alternative splicing transcripts, transcript-specific primers (Supplementary Table 5) were designed to amplify corresponding DNA isolated from B73 seedlings and cDNA derived from B73 pollen RNA. Conserved PME/PMEI domains and gene structures producing validated transcripts were drawn and displayed using the Gene Structure Display Server60 (GSDS2.0; http://gsds.cbi.pku.edu.cn/index.php).
Sequence alignment of ZmPME/PMEIs domain was conducted by T-Coffee (http://tcoffee.org/503/index.html), 3D structures were predicted through I-TASSER61 (http://zhanglab.ccmb.med.umich.edu/ITASSER/) and visualized by Chimera1.13.1 (http://www.cgl.ucsf.edu/chimera/).
Phylogenetic and multiple alignment analyses
The PME/PMEI protein sequences were aligned using ClustalX 2.062 (http://www.clustal.org/clustal2/) with the default parameters. Phylogenetic tree was drawn with the neighbor-joining method using software MEGA7.063 (molecular evolutionary genetics analysis, https://www.megasoftware.net/) using pairwise deletion; 1,000 replicates were used for bootstrap analysis and the cut-off value was 50%. The information of PME/PMEIs genes of rice and Arabidopsis were obtained from two report of Yang et al.9 and Wang et al.12, and the protein sequences were downloaded from the Arabidopsis Information Resource (TAIR; https://www.arabidopsis.org/) and the Rice Genome Annotation Project websites (http://rice.plantbiology.msu.edu/analyses_search_locus.shtml).
We used the MEME system (http://meme.sdsc.edu/meme/itro.html) to identify conserved motifs with parameters set as: number of repetitions, arbitrary; maximum number of patterns, 20; optimal width of the motif, between 6 and 50 residues64.
Calculation of synonymous (Ks) and non-synonymous (Ka) substitutions
To identify homologous pairs of genes, the transcript sequences of the ZmPME/PMEIs were investigated by BLASTN searches65. Paralogous pairs within the genome of maize were defined as follows: the aligned sequences were longer than 300 bp and shared identities ≥40%66. If the amino acid shorter than 300 bp, the aligned region had an identity ≥60% and the alignment length covered ≥50% of the gene were defined paralogous pairs.
Gene ontology (GO) annotation
The translated ZmPME/PMEIs protein sequences were annotated using the Blast2GO5.2.4 program to assign GO terms67 (http://amigo.geneontology.org/amigo/term/). GO analysis e-value is 1.0E-6. GO terms are provided under three main categories, biological process, cellular component, and molecular function.
Localization of fluorescent protein-tagged ZmPMEI16
Full-length ORF of ZmPMEI16 was isolated by PCR using primers ZmPMEI16-pM999-F (5′-AGCAGATCTATCGATGAATTCATGGGGCAAGCCTACCCA-3′) and ZmPMEI16-pM999-R (5′-TCCTTTGCCCATGGCTCTAGATCATATCATGTTTGCAAGCG-3′). The resulting fragment was digested with EcoRI and XbaI and inserted between the corresponding sites of pM999-EGFP (provided by professor Liwen Jiang), which express an engineered version of the green-fluorescent protein (GFP) under the control of the cauliflower mosaic virus 35S promoter. The plasmid pZmPMEI16-GFP and pM999-EGFP were used for transient expression experiments in maize protoplasts68. Samples were analyzed by confocal laser scanning microscopy using a Leica TCS-SP8 operating system as described by Ravanel et al.69.
Expression analysis of the ZmPME/PMEI genes in different tissues
To investigate the spatiotemporal expression patterns of the ZmPME/PMEI genes, RMA-normalized data for ZmPME/PMEI genes were downloaded from PLEXdb (http://www.plexdb.org/). A heat map was produced by Heml 1.0.3.7- Heatmap illustrator.
Semi-quantitative reverse transcription PCR (semiq-RT-PCR)
Total RNA of the B73 inbred line was extracted using the Trizol reagent (Invitrogen, USA) according to the manufacturer’s recommendations. In addition, anthers on tassels (about to exsert from the upmost leaves) were collected from sterile and fertile plants of a backcrossing population derived from a maize S-type CMS, and RNA was also extracted using the same method. First-strand cDNA was synthesized from 0.05–5 μg of total RNA (20 μL reaction volume) using TransScript First-Strand cDNA Synthesis Super Mix (TransGen Biotech). All gene-specific primers were designed by primer 3 (http://primer3.ut.ee/) as shown in Supplementary Table 5. The maize gene Actin1 (GenBank ID: NM_001155179) was used as an internal control. The semiq-RT-PCR assays were repeated for two or three times (biological replications).
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Acknowledgements
We thank Liwen Jiang (The Chinese University of Hong Kong, China) for providing the plasmid pM999-EGFP. This research was supported in part by the Key Research and Development Program of China (No. 2016YFD100804), and a project (No. 2662015PY220) of the Fundamental Research Funds for the Central Universities of China.
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B.Y., J.Z. and Y.Z. designed the study. P.Z., H.W., X.Q. and K.C. performed the experiments. P.Z. and B.Y. wrote the manuscript.
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Zhang, P., Wang, H., Qin, X. et al. Genome-wide identification, phylogeny and expression analysis of the PME and PMEI gene families in maize. Sci Rep 9, 19918 (2019). https://doi.org/10.1038/s41598-019-56254-9
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DOI: https://doi.org/10.1038/s41598-019-56254-9
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