With approximately 1,500 extant species, freshwater crabs (Decapoda: Brachyura) are among the most diverse decapod crustaceans. Nevertheless, their fossil record is extremely limited: only Potamidae, Potamonautidae and Trichodactylidae are reported up to the Eocene of the Neotropics so far. This work documents unusually large decapod claws from the Upper Cretaceous (Campanian) continental deposits of Velaux and vicinity (southern France), in close association with large vertebrate remains. In addition to (1) the systematic assignment of these claws, the study addresses (2) the salinity trends in the deposit environment from its faunal assemblage and the elementary chemical patterns of fossils, and (3) the likely scenario for their auto/allochthony in the Velaux fluvial system. These claws belong to a new taxon, Dinocarcinus velauciensis n. gen. n. sp., referred to as Portunoidea sensu lato, a group of “true” crabs nowadays linked to marine systems. However, the faunal assemblage, the claw taphonomy and the carbonates Y/Ho signatures support their ancient freshwater/terrestrial ecology, making them the oldest reported continental brachyurans and extending the presence of crabs in freshwater environments by 40 Ma. Either as primary or as secondary freshwater crabs, the occurrence of these portunoids in Velaux is an evidence for the independent colonizations of continental environments by multiple brachyuran clades over time, as early as the Campanian.
With approximately 1,500 extant species1, freshwater brachyuran crabs (Decapoda: Brachyura) are among the most diverse decapod crustaceans. Nevertheless, their fossil record is extremely limited. Representatives of three families were identified unequivocally as fossils, including Potamidae Ortmann, 18962, Potamonautidae Bott, 19703 and Trichodactylidae H. Milne Edwards, 18534,5,6,7. Articulated exoskeletons of fossil freshwater crabs are rare7,8,9,10,11,12,13,14, although isolated cheliped fingers are much more frequent, but difficult to evaluate taxonomically9,15,16,17,18. Up to now, Tanzanonautes tuerkayi Feldmann et al.13 (Potamonautidae) from the Oligocene of Tanzania (ca 30 Ma) is the oldest fossil record of a freshwater brachyuran in the Old World and there is no record of Potamidae older than early Miocene9. The oldest record of freshwater crabs is from the middle Eocene of the Amazon Basin (ca 40 Ma) and belongs to the family Trichodactylidae7, a group of crabs that likely colonized freshwater habitats independently from potamoids, as indicated by morphology19 and molecular phylogeny20.
The present paper reports the remains of brachyuran crabs from fluvial Late Cretaceous (late Campanian; ca 72–74 Ma) localities of southern France (Velaux-La Bastide Neuve and vicinity), fossilized in association with vertebrate remains. Close associations of different and diverse fossil organisms may both (1) be the result of a long-distance transport of allochthonous remains or (2) testify of local biocoenoses for which members of quite restricted ecosystems have deposited altogether. These claws are of exceptional large size compared to most Late Cretaceous marine crab claws; and interestingly do not conform to the morphology of any extant freshwater crab family. The presence of presumably freshwater crabs in Campanian deposits is quite unexpected and represents the earliest record of the colonization of freshwater environments by brachyuran decapod crustaceans. It roughly doubles the previously oldest evidence of 40 Ma, and would further support the independent invasion of freshwater environment by several distinct brachyuran lineages6,21,22.
The study aims at (1) proposing a systematic assignment for these claws, (2) characterizing the actual salinity trend of their depositional environment, based on the channel fauna assemblage and elementary chemical patterns of fossils and (3) identifying the relevant taphonomic scenario for the presence of crab claws within a fluvial system. As all these approaches support a freshwater or terrestrial signature for the paleoenvironment of these large-clawed brachyurans; we then discuss the implications for presumed multiple invasions of freshwater habitats by crustacean decapods over time.
Velaux-La Bastide Neuve Channel
Velaux-La Bastide Neuve is located in the western part of the Aix-en-Provence Basin, Southeastern France. K-Ar dating of the locality was attempted based on glauconites collected from sandstones23, but these minerals are clearly reworked from lower Aptian marine limestones and therefore useless for dating the site23. Magnetostratigraphic analysis of the deposits, however, correlates with the normal chron of chron 3223, corresponding to an age of 71.6 to 74 Ma24. Along with correlations with charophytes and dinosaur eggshell biozones, a late Campanian age for the locality may confidently be proposed23,25,26,27. The fossil site is mostly known for its vertebrates assemblage, recovered from three different sedimentological sequences and corresponding to newly described dinosaur (titanosaurid sauropod26,28; rhabdodontid ornithopod29) and pterosaur (azhdarchid pterosaur30) taxa, as well as eusuchian crocodilians27. Apart from the diapsids, vertebrates consist of disarticulated pleurodiran and cryptodiran turtles, disconnected remains of sarco-/actinopterygians, and chondrichthyan teeth. Freshwater bivalves (Unio) and gastropods (Physa, Melania)30, macro-remains of angiosperm plants and charophytes complete the whole fossiliferous assemblage together with the herein described crustacean remains. The lithological section consists of 16.3 meters of alternating sandstones, siltstones – including palaeosols – and mudstones. Lacustrine limestones occur in the uppermost part of the section. The succession was deposited in a fluvio-lacustrine environmental setting. The sedimentology of the site together with the fossil assemblage indicates a likely freshwater setting for the deposits. The succession of conglomeratic sandstones, siltstones (including palaeosols), mudstones and lacustrine limestone on top of the stratigraphic section indicate sedimentation in, respectively, a low-energy fluvial channel, channel levees, alluvial plain and lake23. Given the proximity of Velaux to the palaeo-coast during the Late Cretaceous31,32, occasional marine incursions are not excluded, even though they were not recorded at Velaux-La Bastide Neuve nor at other fossiliferous localities of the same age in the region23,25.
The studied material (Tab. 1) consists of seven (partial) claws and associated vertebrate remains collected from sequence 2 of the sedimentary succession of Velaux-La Bastide Neuve locality and one from the close locality of Rognac-Les Frégates (about four km from Velaux, corresponding to similar layers). Specimens are housed in the palaeontological collections of Velaux municipal institutions (Musée du Moulin Seigneurial/Velaux-La Bastide Neuve: MMS/VBN.00.004, 09.69e, 12.A.006, 02.94, 09.43, 09.132d, 12.A.003) and of the Muséum d'Histoire naturelle d'Aix-en-Provence, France (MHN AIX PI 1991.1, coll. Valentin).
Preliminary remarks: Taxonomic assignment of isolated claws of brachyuran crabs at the species or genus level is difficult, if not impossible in many cases. If direct comparisons with extant taxa is straightforward and often helpful for identifying Pliocene and Pleistocene brachyurans33,34,35,36, this task becomes more complicated when working with material of Miocene age37,38,39,40. The taxonomic evaluation of isolated fossil cheliped fingers is of course further challenging. Erecting new taxa based on isolated brachyuran chelae alone has not been attempted yet, although this method is regarded as valid in other decapod groups, including paguroid hermit crabs41,42,43, erymid lobsters44,45 or callianassid ghost shrimps46,47. If working with distinct claw morphologies, the erection of new taxa can be done48. Alternatively, parataxonomy can be used49.
We assume that the morphology and also the size of the studied claws from the two localities Velaux-La Bastide Neuve and Rognac-Les Frégates are distinct enough to warrant the validity of the new form genus Dinocarcinus. Based on the general morphology of its claws, we include this form genus within Portunoidea sensu lato50. For portunoid crabs the presence of large proximal molariform tooth on the dactylus on one of the chelae, and serial (often bi- and tri-lobed) conical teeth on the dactylus and fixed finger of both chelae are typical50,51. It should, however, be mentioned that the presence of a massive molariform tooth is not unique for portunoids, but it can be found in a number of heterotreme superfamilies (sensu Ng et al.52). Our material shows some affinities to selected representatives of Xanthidae, although, in these crabs, finger usually is somewhat shorter than manus53 and generally exhibit a different pattern of serial teeth50. In this respect, the Dinocarcinus material is not preserved sufficiently enough to allow detailed comparison of the cheliped dentition of various portunoid or xanthoid crabs. Yet, its taxonomic features (as mentioned above) point out affinities with portunoids, although this attribution has to be considered as preliminary. In this sense, Dinocarcinus velauciensis is kept in open nomenclature.
Etymology: Denoting the actual association with dinosaur (ornithopodan) remains.
Type species: Dinocarcinus velauciensis n. gen. n. sp.
Diagnosis: Chelae large and massive. Fingers gaping, arched, with strong teeth, proximal tooth molariform. Fixed finger dorsal surface with single “pitted groove”, palm surface smooth, articulation with dactylus oblique, prominent.
Remarks: The morphology of the claws, namely heavily calcified fingers, strong molariform teeth and grooved fingers, are typical for several eubrachyuran crabs. There are only few representatives of podotreme clades which grew to large sizes, with particular exceptions of Dakoticancridae and Cenomanocarcinidae, which could have claws comparable in size to Dinocarcinus velauciensis n. gen. n. sp. Figured claws of Avitelmessus Rathbun, 192354, show the following54,55,56: the fingers’ (distal) teeth are not molariform, fingers are less robust and less strongly calcified than the remains studied herein. The claws of Avitelmessus are more curved, the palm is longer than the fingers, both palm and fingers have crests and grooves; the fingers tips are hooked; distinguishing it easily from Dinocarcinus n. gen. The palaeocorystoid Cenomanocarcinidae could attain large sizes and had massive claws. The claws of Cenomanocarcinus Van Straelen, 193657 are figured by Guinot et al.58 (Fig. 6) and are characterized by spinose claws, flattened in cross section, a slightly downturned fixed finger, spines along the upper margin of the claw and dactylus, and hooked tips. Compare also the very large claws of ‘Oncopareia’ heterodon Bosquet, 185459, now considered to be a palaeocorystoid (in Jagt et al.60: plate 5). As discussed above, the claw morphology of Dinocarcinus velauciensis n. gen. n. sp. does not match that of the Dakoticancroidea, Palaeocorystoidea, or any known Podotremata.
Within Eubrachyura, the robust, strongly calcified fingers, overall claw shape, and molariform teeth, match that of the Portunoidea. This large group of overall large-sized crabs have several Mesozoic occurrences, and some of them in large sizes. Ophthalmoplax Rathbun, 193561, now considered a representative of Macropipidae62 has a great size range, from very large Ophthalmoplax brasiliana Maury, 193063, to rather small O. minimus Osso64. Their claws [compare65 (Figs. 3.2, 3.3, 4.2, 4.13, 4.14) with66 (Fig. 6.7)] are spinose, keeled, with major claws showing a large bulbous proximal tooth index inferred to be used for shell breaking67. These specialized claws can be easily distinguished from the more simple, unarmed claws of Dinocarcinus n. gen. Eogeryon Osso66 (Cenomanian of Spain) is assigned to the Portunoidea in its own family (Eogeryonidae Osso66). Geryonidae Colosi, 192368 and Eogeryonidae are considered as early diverging families within Portunoidea. Eogeryon is characterized by large claw size relative to the carapace, equal ratio palm-fingers, with strongly calcified fingers with molariform teeth, and grooved fixed finger. Its claw morphology is typical of that of Portunoidea, and compared with that of Styracocarcinus meridionalis (Secrétan, 196169) from the?Campanian of Morocco. Claws of Litoricola macrodactylus (Van Straelen, 192470) from the Paleocene of southern France and Northern Spain, are highly comparable with those of Dinocarcinus n. gen., however they show a bulbous proximal crushing tooth on the dactylus of the major claw. Also, the fingers in Dinocarcinus n. gen. are more gaping than those of Litoricola.
The claw morphology of Dinocarcinus n. gen. shows few diagnostic characters for superfamily level assignment (Portunoidea), namely heavily calcified claws, a grooved fixed finger, molariform teeth, palm, and fingers subequal in length, and blunt, non-hooked fingertips (Fig. 2). More accurate assignation is not possible at this point. An early diverging position within Portunoidea is possible considering morphology, geologic age, large size, and similar families occurring at that time.
Of note is yet another occurrence of the Late Cretaceous crab, namely Megaxantho zoque Vega et al., 2001 from the Maastrichtian of Mexico71. Its sheer size and general morphology of chelae is reminiscent of Dinocarcinus; both taxa, however, differ from each other in a number of characters. Cheliped dentition of Megaxantho was directly compared to portunoids67,71. Originally, Megaxantho was classified within Xanthidae; nevertheless, its attribution was later questioned and its assignment to Xanthoidea incertae sedis was finally suggested5.
Type material: Holotype: MMS/VBN.00.004; Paratypes 1–4: MMS/VBN.02.94, 09.132d, 12.A.003, 12.A.006 (see Table 1).
Etymology: From Velaux-La Bastide Neuve, Bouches-du-Rhône, the type locality.
Diagnosis: As for genus.
Description: Only claws known; claw very large (approximately 85 mm for holotype MMS.VBN.00.004), massive, outer surface flat. Palm subrectangular, slightly longer than high, slightly longer than fixed finger. Fixed and movable fingers inwards curved, clearly gaping. Lower propodus margin curved, weakly convex. Upper (cutting) margin of fixed finger straight. In total, 4 strong teeth on fixed finger. Proximal tooth massive (T1/t1), molariform in both fingers. Surface of molariform proximal tooth bulbous on fixed finger, flat on dactylus. Fixed finger dorsal surface with a single “pitted groove”. Articulation dactylus-propodus prominent, oblique. Both finger tips sharp, pointed. Dactylus upper and lower margin curved in dorsal view; dactylus cross-section flatter than propodus cross-section. Dactylus more strongly curved in dorsal view than fixed finger. Dactylus margin at approximately 100 degrees at upper (cutting) margin of fixed finger. Fingers strongly calcified, cuticle surface (as preserved) covered with microscopic dense, flattened granules.
The associated vertebrates
Four of the brachyuran claws were discovered in close association with vertebrate remains. The most complete and larger specimen (MMS/VBN.00.004) is fossilized onto a vertebra that closely resembles a posterior cervical vertebra of a rhabdodontids Iguanodontia (Fig. 1a,b). Rhabdodontids are represented at Velaux by the genus Matheronodon Godefroit et al.29 and in other Late Cretaceous localities from southern France, by Rhabdodon Matheron72. MMS/VBN.09.69e is found in close sedimentary association with the plastral plate of a terrestrial turtle (Solemys de Lapparent de Broin & Murelaga73, Solemydidae) (Fig. 1c,d). MMS/VBN.12.A.006 is preserved in a 50 cm large block that also contains a turtle plastral plate (Polysternon Portis74, Bothremydidae), a rhabdodontid tooth and centrum, as well as hybodontid shark teeth (Fig. 1e,f). MMS/VBN.12.A.003, figured isolated (Fig. 3d), has been extracted from a comparable block, also containing a crocodylomorph skull, a rhabdodontid tooth, as well as a partial titanosaurid dorsal vertebra showing preserved ossified tendons.
Taxonomic/ecological diversity in the sequence 2 of the channel
The freshwater palaeoenvironment of the channel in sequence 2 is strongly supported not only by sedimentological evidence23, but also by the most recently collected taxonomic assemblage itself. The fossil remains (n = 308 specimens) consist of 42.5% of strict terrestrial/aerian Avemetatarsalia indicative of an absolute continental faunal assemblage (Fig. 4). Aquatic and semi-aquatic taxa consist of families and genera, whose previous depositional record is strongly anchored in freshwater environments. Among archosaurs, the hylaeochampisdae crocodylomorph Allodaposuchus Nopsca27,75) has so far been reported from fluvial inner/lacustrine-interpreted environments76,77,78 and once in a more coastal swampy area79. These are associated to a small amount of Globidonta, which, as members of Alligatoroidea, would have secondarily lost salt glands and therefore have been also restricted to freshwater settings80. Other highly abundant sauropsids in Velaux are chelonians, equivalently represented by Bothremydidae (Polysternon) and Solemydidae (Solemys, Fig. 4). If the former family is recognised as the most abundant and diverse European group of freshwater and coastal turtles in the uppermost Cretaceous81, Polysternon is only reported from estuarian to alluvial sediments and its sister-genus Foxemys Tong et al.82, is exclusively known from freshwater localities83,84. The case of Solemydidae is even more compelling because their dermal skeleton (skull osteoderms) is highly supportive of a strict terrestrial life habit85 rather than any degree of amphibious lifestyle. The identified chondrichtiyan teeth correspond to a unique hybodontid genus: Meristonoides Case & Capetta86, which presence at Velaux has been briefly questioned (Cuny pers. comm. in87), although it is well accepted that hybodontid sharks are common in fluvial ecosystems in the Cretaceous88. The least abundant remains at Velaux belong to an aquatic sarcopterygian identified as Axelrodichthys megadromos Cavin et al.89 (Cavin pers. comm.). The only known occurrence of this mawsoniid coelacanth is from another French Campanian lacustrine deposit88, confirming the unequivocal freshwater nature of the fauna from the sequence 2, which includes the brachyuran claws in the Velaux-La Bastide Neuve channel (Fig. 4).
As for rare-earth-elements, the Y signature of limestones and carbonate concretions can be used as a recorder of ancient seawater signatures90,91,92. Y and Ho concentrations can be compared because these elements are chemically similar in charge and ionic radius, and suggested to evolve similarly over time in few terrigenous diagenetic environments91. Consequently, measured on fossil carbonates, Y and Ho concentrations can partly document their relative original intake into abiogenic/biogenic carbonates and therefore inform on marine/non marine pre-diagenetic environments of formation. The carbonates of MMS/VBN.09.69e-claw display a Y/Ho ratio of 33.06 (Y = 8.61 ppm; Ho = 0.26 ppm). The ratio in MMS/VBN.09.69e-sediment is a bit lower with 29.86 (Y = 11.3 ppm; Ho = 0.37 ppm). Marine waters are known to have a quite constant Y/Ho ratio around molar 90–110, decreasing with depth92,93. Consequently, neither the sandstones hosting the claws, nor the claw carbonate could have been deposited and/or formed in marine conditions. Apart from the marine realm, Y/Ho data characterizing formally typical estuarine or fluviatile environments are hitherto not commonly reported94. Nozaki et al.94 evidenced from the study of Japanese fluvial systems that Y and Ho concentrations were constantly decreasing with the salinity, with Ho removed from seawater twice as fast as Y owing to differences in surface complexation behaviour. Unfortunately, Y and Ho absolute concentrations (which we could expect to interpret from the studied material) depend on biological and taphonomic factors of integration that simply cannot be estimated here. Consequently, the salinity of the studied channel cannot be assessed from the chemistry. In all cases, the observed Y/Ho ratio formally excludes a marine/costal pattern and seems to distinguish strongly from it.
Taphonomy of the assemblage
The crab chelae studied herein were recovered from the fluvial channel sediments from sequence 2, which correspond to lenticular conglomeratic sandstone (Fig. 5a,b). This association of elements belonging to diverse – aquatic and terrestrial – vertebrate taxa probably results from the transport of decayed carcasses originating from diverse environmental settings in a river channel. The associated bones and tooth elements are found disarticulated. The preservation of some complete bony elements, like crocodylomorph skulls, argues for their relatively short timing of decay and transport, consistent with the most common reports of the group in fluvial/ inner lacustrine type of environments. The Velaux taphonomy would indicate a local riverine system with a low-enough energy to allow the deposit of small millimetric elements like Meristonoides teeth23. An option could be that large elements (large appendicular bones, carapace portion and skulls) would have acted as obstacles for smaller ones in a more intermediate-energy flow configuration, resulting in a mixture of elements of different sizes and spatial origins23. In both cases, as in any continental water system, the deposit must have occurred from upstream to downstream implying that all the remains in sequence 2 must have belonged either to original local fluvial living-individuals (sedimentary context) or to upstream/even more terrestrial ones (floodplain and levees). Consequently, the presence of strictly freshwater lineages (Globidonta crocodylomorphs), would restrict the salinity inside this part of the channel to a minimum, implying that crabs must have been living either in terrestrial or freshwater aquatic habitats. The spatial distribution of the crabs within the conglomeratic sequence (sequence 2 on Fig. 5c,d) is heterogeneous: they are in most cases horizontally spaced by several dozens of centimeters. The absence of further connection of the brachyuran remains (e.g. with manus/carpus) or other body parts than dactyli and/or propodi is poorly informative on transport/exposure time experienced by claws given the admitted proclivity of chelae from decapod crustaceans to preserve the best after years (see95,96 for brachyurans). Additionally, the sand-/siltstones that yielded the claws do not provide high probabilities for carapaces nor full exoskeletons to be preserved in connection (as mudstones might have for instance).
Decapod crustaceans in Cretaceous continental assemblages
Decapod crustaceans, such as shrimps and ghost shrimps, have been mentioned in assemblages of continental vertebrates, including dinosaurs97,98,99. The oldest report involving brachyuran crabs is from a Cenomanian mangrove coastal environment (Bahariya Formation, Egypt)100. In this case, the authors even suggested a scavenging behaviour for these crabs based on their relative proximity to bony remains. Likewise, the Velaux-La Bastide Neuve channel may have provided a favorable environment for the rise of invertebrate scavengers. However, neither the claws shape nor their important size could inform on a specific diet, nor on crabs interactions with reported members of this ecosystem, although some modern freshwater durophagous crabs have dentition developed in a similar fashion101,102. Interestingly, it is worth stressing that consumption behaviours of decapod crustaceans by megaherbivorous dinosaurs have been reported from the Campanian of two North American formations103. In that case, in-situ coprolites of ankylosaurs, brachylophosaurs and neornithischians revealed undetermined crustacean cuticles associated to fragments of likely rotted wood fragments. This led the authors to suggest these crustaceans were consumed when they were sheltering inside dead plant logs. The cuticles could not be identified as belonging to a specific order, but were mentioned as possibly corresponding to claws reported from surrounding continental middle Campanian formations104,105,106. However, the figured corresponding claws, referred as large freshwater crab ones, clearly actually correspond to anomuran – and no brachyuran – chelae105 (see p. 583, Fig. 26.28). However, smaller (up to 3 cm) brachyuran-like dactyls are mentioned in the Maastrichtian of Fox Amphoux (Var, France)106 and the Hateg Island (Transylvania, Romania)107. In the latter case, the authors question the allochthonous marine origin of the claws, moved in as preys, by other animals. The consistent autochthony of all other invertebrates in the channel (Unio, Physa, Melania), and the Y/Ho carbonates displayed by claws, limit our interpretation to the most parsimonious assertion: it is rather unlikely that claws were moved into the Velaux fluvial system, as preys, from further marine environments.
Decapod crustaceans in freshwater habitats
Decapod crustaceans inhabit virtually all water-influenced habitats, including freshwater bodies, from streams and rivers to ponds and lakes, and even caves. In fact, representatives of a number of originally exclusively marine decapod clades have successfully invaded freshwater and/or terrestrial habitats. In this respect, the following listing of freshwater decapods is not meant to be exhaustive but rather illustrative of the independent colonization of freshwater habitats within this group of crustaceans.
Among caridean shrimps, more than 650 species, making a full quarter of all described species, inhabit freshwater108, with representatives of Merguia Kemp109, being semi-terrestrial110. With approximately 650 species, virtually all crayfish are freshwater animals111. Many axiideans and gebiideans are able to tolerate pretty low salinity conditions. The callianassid Lepidophthalmus Holmes112, is even able to tolerate freshwater environments113 and Lepidophthalmus turneranus (White, 1861114) has been reported to migrate up rivers in West Africa115. Among anomurans, a rather speciose family Aeglidae Dana, 1852116, is strictly freshwater117,118,119. The majority of freshwater decapod crustaceans, however, consists of Brachyura with one fifth (>1 280 species) of all120.
Primary (so called true) freshwater crabs are those that have adopted freshwater, semi-terrestrial or terrestrial modes of life, and are able to complete their life cycle independently of the marine environment1. However, there are a number of brachyuran crabs able to live in freshwater habitats that include euryhaline species or secondary freshwater species from primarily marine brachyuran families1. These do not have direct development in their life cycle, which is typical for true freshwater crabs. Today there are five primarily freshwater families of brachyuran crabs with no marine species120, i.e. Gecarcinucidae, Potamidae, Potamonautidae, Pseudothelphusidae, and Trichodactylidae, whereas there are also numerous secondary freshwater, semi-terrestrial and terrestrial species among Majoidea (Hymenosomatidae), Goneplacoidea (Goneplacidae), Grapsoidea (Gecarcinidae, Sesarmidae, Varunidae) and Ocypodoidea (Ocypodidae)1. Many grapsoids invade or even wholly inhabit freshwater habitats. Some varunids, including representatives of Eriocheir De Haan, 1835121, and Varuna H. Milne Edwards, 1830122, not only enter estuaries, but are also found further up in rivers1. Sesarmids (Sesarmoides, Labuanium, Karstarma) can be completely adapted to freshwater, the latter being semi-terrestrial120,123,124,125, whereas Geosesarma De Man, 1892126 is found in terrestrial habitats118,119.
All primary freshwater crabs are entirely free from the need to enter seawater. As for the fossils from Velaux and surroundings newly described herein, it cannot be decided unequivocally whether representatives of Dinocarcinus n. gen. were able to complete their life cycle in the freshwater habitat or/and had direct development. The sheer size and number of claw fragments, however, may prove that crabs from the Late Cretaceous of Velaux were not only an occasional element of the respective environment, but rather a natural part of the assemblage suggesting that they were fully adapted to freshwater environment. Apparently, the remains belonged to adult animals, so it is more likely they were primary freshwater crabs.
Freshwater decapods in the fossil record
Fossil freshwater decapods are exceedingly rare in comparison to their marine relatives. Fossil crayfish are represented only by a handful of occurrences reported so far (123,124,127,128,129,130,131 and references therein), the oldest possibly coming from the Triassic of Utah128. Fossils of freshwater caridean shrimps are similarly rare127,129,132,133,134,135, the oldest being reported from the Early Cretaceous of Spain129 and China135. Interestingly, the only fossil representative of nowadays strictly freshwater anomurans, the family Aeglidae, comes from marine strata136. Fossil freshwater brachyurans are limited to a number of occurrences of isolated claws14,20,21,22,23 and several fossils exhibiting preserved carapace12,13,14,15,16,17,18,137. The oldest occurrences of undisputed primary freshwater crabs is from the middle Eocene (late Lutetian/early Bartonian) of the Amazon Basin, recently reported by Klaus et al.7 who described isolated claw elements of Trichodactylidae. A recent report of Alontecarcinus buratoi De Angeli & Caporiondo, 2019137 from the middle Eocene (Bartonian) of Italy, being interpreted as the oldest representative of Potamidae, should be taken with caution because of its rather unique carapace sculpture, unknown in potamids (Sebastian Klaus, pers. comm 2019). Moreover, A. buratoi is described from a setting suggesting a brackish environment, which contradicts the ecology of at least extant primary freshwater crabs (as discussed above). In this respect, Dinocarcinus velauciensis n. gen. n. sp. reported herein is the oldest occurrence of freshwater brachyuran crabs, exceeding previous reports by approximately 40 million years. For now, it is unclear whether Dinocarcinus belonged to primary or secondary freshwater crabs. It is, however, of note that recent advances in resolving the phylogeny of primary freshwater crabs suggest their early divergence in brachyuran evolution138. Both, potamoid and portunoid crabs, i.e. primary freshwater and marine crabs, are suggested to be roughly equally old.
Multiple invasions into freshwater habitats
From the discussion above it is clear that several lineages of decapod crustaceans independently invaded freshwater habitats, including dendrobranchiatans139, carideans, axiideans, astacideans, anomurans and brachyurans11,24,118,140. The enigmatic Tealliocaris Peach, 1908141, considered by some authors as a decapod crustaceans142 (but see also143), might represent yet another freshwater lineage. And among carideans, at least palaeomonoid, atyoid, and alpheoid shrimps independently invaded freshwater environments108. Moreover, presumed multiple invasions of freshwater habitats by some Macrobrachium Bate, 1868144 shrimps were also suggested145. The sparse fossil record of freshwater shrimps does not allow relevant time estimation of colonization of freshwater habitats; however, fully freshwater shrimps are known from the Early Cretaceous (Barremian) onward129,135. Crayfish represent a monophyletic group146 with the oldest fossil representatives known from the Late Triassic of Utah128. As for brachyuran freshwater crabs, there are two independent lineages. The Old World primary freshwater crabs are monophyletic11,24,120,147, whereas Neotropical Trichodactylidae have a separate phylogenetic origin24,148. Based on the morphology of its chelae, Dinocarcinus velauciensis n. gen. n. sp. cannot be referred to any of the extant primary or secondary freshwater families mentioned above suggesting that it may represent yet another independent “attempt” to colonize freshwater environment besides the two primary freshwater crab clades recognized today, i.e., Potamoidea and Trichodactyloidea24,120. Interestingly, the oldest fossil representatives of both clades come from the middle Eocene12,137, although the oldest putative potamid132 needs further investigation (see above). The geographic distribution of modern primary freshwater crabs speaks for independent invasions of the limnic habitat rather than for a Gondwanan vicariance11,149, contrasting with the diversification of crayfishes: the fossil record and modern distribution of the latter clade can be explained by the breakup of Pangaea and disassembly of Gondwana and Laurasia140. The present occurrence of Dinocarcinus further supports independent colonization of freshwater habitats by brachyurans. Based on molecular clock estimates, Daniels et al.149 suggested that the radiation of Afrotropical freshwater crab taxa occurred during the Early Cretaceous, whereas the age of the African Potamonautidae clade was given with 75–73 Ma (Campanian). From the fossil record of the modern freshwater families alone, such timing cannot be apprehended; however, the discovery of fossil crabs from Velaux-La Bastide Neuve illustrates that brachyuran crabs attempted to colonize freshwater habitats in the Old World at least from the Campanian onwards.
One of the key processes driving freshwater crab diversification is likely allopatric speciation resulting from geographic isolation, often coupled with habitat heterogeneity and numerous ecological niches and microhabitats resulting from the complicated topography and hydrology of freshwater environments1. During the Campanian, Dinocarcinus velauciensis inhabited Europe, which was at its time an archipelago rather than a proper landmass37,64,75,136,150. Based on the material from Velaux and Rognac described herein, we suggest that the freshwater habitats of islands in the Tethyan epicontinental sea were colonized by marine portunoids during the Late Cretaceous. Nowadays, most secondary freshwater brachyurans have a marine larval development and would reach inland habitats more likely as adults. This might also have been the case for Dinocarcinus velauciensis.
Dinocarcinus velauciensis n. gen. n. sp. from the late Campanian of Southern France, belongs to Portunoidea sensu lato, a group of “true crab” that are nowadays intimately linked to marine systems. The sedimentological context, faunal assemblage and taphonomy of these fossils, as well as the Y/Ho ratio of their carbonates indicate an ancient freshwater or terrestrial ecology. This makes them the oldest freshwater/terrestrial brachyurans ever reported, extending the existence of freshwater crabs by 40 Ma. In this Campanian ecosystem, “true” crabs were associated to terrestrial vertebrates, including non-avian dinosaurs. Although they were likely well adapted to this environment, it cannot be decided whether Dinocarcinus was able to complete its life cycle in the freshwater habitat or/and had direct development. Its occurrence in the Late Cretaceous of Velaux-La Bastide Neuve, is an evidence for the independent colonizations of freshwater environments by multiple Brachyura clades over time, beside that of modern primary freshwater crabs (Potamoidea, Trichodactyloidea). It also supports the molecular clock estimation of an Early Cretaceous start for the radiation of Afrotropical freshwater crab taxa (just appearing in the Late Cretaceous), with the evidence of brachyuran crabs colonizing freshwater habitats as early as the Campanian.
Material and Methods
The elementary composition of a brachyuran claw and of its surrounding matrix were investigated for their Y/Ho ratios. One gram of each was sampled on MMS.VBN.09.69e (claw/sediment). For the claw material, the basis of the propodus embedded in the matrix was mechanically sampled to preserve the connection between the claw and the turtle plate. Samples were microground and analysed for their composition in minor elements (in µg/g) normalized to PAAS, using ICMPS at the Service d'Analyses des Roches et des Minéraux of the CRPG, Vandoeuvre-lès-Nancy, France.
All data needed to evaluate the conclusions in the paper are present in the paper and supplementary information.
Yeo, D. C. J. et al. In Freshwater animal diversity assessment 275–286 (Springer, 2008).
Ortmann, A. Das System der Decapoden-Krebse. Zool. Jahrbücher, Abteilung für Syst. Geogr. und Biol. Thiere 9, 409–453 (1896).
Bott, R. Die Süßwasserkrabben von Europa, Asien und Australien und ihre Stammesgeschichte. Abh. senckenb. naturforsch. Ges 528, 1–338 (1970).
Milne Edwards, H. Mémoires sur la famille des Ocypodiens, suite. Ann. des Sci. Nat. Zool. Ser. 3 20, 163–228 (1853).
Schweitzer, C. E., Feldmann, R. M., Garassino, A., Karasawa, H. & Schweigert, G. Systematic list of fossil decapod crustacean species. in Crustaceana Monographs 230 pp, (Brill, 2010).
Klaus, S., Yeo, D. C. J. & Ahyong, S. T. Freshwater crab origins—laying Gondwana to rest. Zool. Anzeiger-A J. Comp. Zool. 250, 449–456 (2011).
Klaus, S., Magalhães, C., Salas-Gismondi, R., Gross, M. & Antoine, P.-O. Palaeogene and Neogene brachyurans of the Amazon basin: a revised first appearance date for primary freshwater crabs (Brachyura, Trichodactylidae). Crustaceana 90, 953–967 (2017).
Szombathy, K. Die tertiären Formen der Gattung Potamon (Telphusa) und ihre paläarktischen Nachkommen. In Annales historico-naturales Musei Nationalis Hungarici, Budapest 14, 281–472 (1916).
Klaus, S. & Gross, M. Synopsis of the fossil freshwater crabs of Europe (Brachyura: Potamoidea: Potamidae). Neues Jahrb. für Geol. und Paläontologie-Abhandlungen 256, 39–59 (2010).
Hyžný, M. A freshwater crab Potamon (Brachyura: Potamidae) from the middle Miocene Lake Bugojno (Gračanica, Bosnia and Herzegovina), with notes on potamid taphonomy. Palaeobiodiversity and Palaeoenvironments in press, 1–7
Glaessner, M. F. Die Dekapodenfauna des osterreichischen Jungtertiars. Jahrb. der Geol. Bundesanstalt 78, 161–219 (1928).
Glaessner, M. F. Dekapodenstudien. Neues Jahrb. fur Mineral. 137–176 (1929).
Feldmann, R. M. et al. A new freshwater crab (Decapoda: Brachyura: Potamonautidae) from the Paleogene of Tanzania. Africa. Neues Jahrb. für Geol. und Paläontologie-Abhandlungen 244, 71–78 (2007).
Pasini, G. & Garassino, A. Unusual scaled preservation samples on freshwater decapods (Crustacea, Decapoda) from the Pleistocene (Late Cenozoic) of Turkey and Kazakistan. Nat. Hist. Sci. 13–18 (2011).
Klaus, S. & Prieto, J. In Advances in freshwater decapod systematics and biology 161–172 (Brill, 2014).
Ng, P. K. L. Fossil brachyuran crabs from the Jambusan Caves (Bau, Sarawak), collected by AH Everett in 1878–1879. Scr. Geol (2014).
Klaus, S., Böhme, M., Schneider, S., Prieto, J. & Phetsomphou, B. Evidence of the earliest freshwater decapod fossil from Southeast Asia (Crustacea: Decapoda: Brachyura). Raffles Bull. Zool. 59, 47–51 (2011).
Klaus, S. et al. A fossil freshwater crab from the Pliocene Tatrot Formation (Siwalik Group) in Northern India (Crustacea, Brachyura, Potamidae). Palaeoworld 26, 566–571 (2017).
Sternberg von, R., Cumberlidge, N. & Rodriguez, G. On the marine sister groups of the freshwater crabs (Crustacea: Decapoda: Brachyura). J. Zool. Syst. Evol. Res. 37, 19–38 (1999).
Tsang, L. M. et al. Evolutionary history of true crabs (Crustacea: Decapoda: brachyura) and the origin of freshwater crabs. Mol. Biol. Evol. 31, 1173–1187 (2014).
Bott, R. Besiedlungsgeschichte und Systematik der Astaciden West-Europas unter besonderer Berücksichtigung der Schweiz. Rev. suisse Zool. 79, 387–408 (1972).
Cumberlidge, N. & Ng, P. K. L. In Decapod crustacean phylogenetics 503–520 (CRC Press, 2016).
Cincotta, A. et al. Integrated paleoenvironmental reconstruction and taphonomy of a unique Upper Cretaceous vertebrate-bearing locality (Velaux, Southeastern France). PLoS One 10, e0134231 (2015).
Gradstein, F. M., Ogg, J., Schmitz, M. & Ogg, G. International Stratigraphic Chart. International Commission on Stratigraphy (2009).
Garcia, G. & Vianey-Liaud, M. Dinosaur eggshells as biochronological markers in Upper Cretaceous continental deposits. Palaeogeogr. Palaeoclimatol. Palaeoecol. 169, 153–164 (2001).
Garcia, G., Amico, S., Fournier, F., Thouand, E. & Valentin, X. A new titanosaur genus (Dinosauria, Sauropoda) from the Late Cretaceous of southern France and its paleobiogeographic implications. Bull. la Société géologique Fr. 181, 269–277 (2010).
Martin, J. E. et al. New specimens of Allodaposuchus precedens from France: intraspecific variability and the diversity of European Late Cretaceous eusuchians. Zool. J. Linn. Soc. 176, 607–631 (2016).
Díez Díaz, V. et al. The titanosaurian dinosaur Atsinganosaurus velauciensis (Sauropoda) from the Upper Cretaceous of southern France: new material, phylogenetic affinities, and palaeobiogeographical implications. Cretac. Res. 91, 429–456 (2018).
Godefroit, P. et al. Extreme tooth enlargement in a new Late Cretaceous rhabdodontid dinosaur from Southern France. Sci. Rep. 7, 13098 (2017).
Vullo, R., Garcia, G., Godefroit, P., Cincotta, A. & Valentin, X. Mistralazhdarcho maggii, gen. et sp. nov., a new azhdarchid pterosaur from the Upper Cretaceous of southeastern France. J. Vertebr. Paleontol. 38, 1–16 (2018).
Scotese, C. R. Atlas of earth history. (University of Texas at Arlington. Department of Geology. PALEOMAP Project, 2001).
Csiki-Sava, Z., Buffetaut, E., Ősi, A., Pereda-Suberbiola, X. & Brusatte, S. L. Island life in the Cretaceous-faunal composition, biogeography, evolution, and extinction of land-living vertebrates on the Late Cretaceous European archipelago. Zookeys 1 (2015).
Kato, H. & Karasawa, H. Pleistocene fossil decapod Crustacea from the Boso Peninsula, japan. Nat. Hist. Res. Spec. Issue 5, 1–31 (1998).
Portell, R. W. & Agnew, J. G. Pliocene and Pleistocene decapod crustaceans. (Florida Paleontological Society, 2004).
Ando, Y., Kawano, S., Komatsu, T. & Niitani, M. Decapod crustaceans from the Pleistocene Oe Formation in Minamishimabara City, Nagasaki Prefecture, Japan. J. Foss. Res. 48, 16–25 (2016).
Ando, Y. & Kawano, S. Decapods from the lower Pleistocene Masuda Formatio in Minamitane-cho, Kagoshima Prefecture, Japan. Bull. Mizunami Foss. Museum 43, 83–92 (2017).
Janssen, A. W. & Müller, P. Miocene Decapoda and Mollusca from Ramsel (province of Antwerpen, Belgium), with a new crab genus and a new cephalopod species. Scr. Geol. 75, 1–26 (1984).
Förster, R. Decapod crustaceans from the Middle Miocene (Badenian) deposits of southern Poland. Acta Geol. Pol. 29, 89–106 (1979).
Förester, R. Decapod crustaceans from the Korytnica basin (Middle Miocene; Holy Cross Mountains, Central Poland). Acta Geol. Pol. 29, 253–268 (1979).
Portell, R. W. Eocene, Oligocene, and Miocene decapod crustaceans. Ann. Carnegie Museum 75, 111–136 (2004).
Schweitzer, C. E. & Feldmann, R. M. New Cretaceous and Tertiary decapod crustaceans from western North America. Bull. Mizunami Foss. Museum 28, 173–210 (2001).
Fraaije, R. H. B., Van Bakel, B. W. M., Iserbyt, A. & Jagt, J. W. M. New extinct Paguroidea (Crustacea, Decapoda, Anomura), with the first example of capsulated setae from the fossil record. Neues Jahrb. für Geol. und Paläontologie-Abhandlungen 262, 247–255 (2011).
Hyžný, M., Fraaije, R. H. B., Martin, J. E., Perrier, V. & Sarr, R. Paracapsulapagurus poponguinensis, a new hermit crab (Decapoda, Anomura, Paguroidea) from the Maastrichtian of Senegal. J. Paleontol. 90, 1133–1137 (2016).
Devillez, J. & Charbonnier, S. Review of the Early and Middle Jurassic erymid lobsters (Crustacea: Decapoda). BSGF-Earth Sci. Bull. 190, 6 (2019).
Devillez, J., Charbonnier, S., Veselská, M. K. & Pezy, J.-P. Review of the Late Cretaceous erymid lobsters (Crustacea: Decapoda) from the Western Tethys. Proc. Geol. Assoc. 128, 779–797 (2017).
Beschin, C., De Angeli, A., Checchi, A. & Zarantonello, G. Crostacei eocenici di Grola presso Spagnago (Vicenza, Italia settentrionale). Stud. e Ric. Assoc. Amici del Museo, Mus. Civ. ‘G. Zannato’, Montecchio Magg. 12, 5–35 (2005).
Hyžný, M. & Klompmaker, A. A. Systematics, phylogeny, and taphonomy of ghost shrimps (Decapoda): a perspective from the fossil record. Arthropod Syst. phylogeny 73, 401–437 (2015).
Hyžný, M. & Kroh, A. Barremian decapod crustaceans from Serre de Bleyton (Drôme, SE France). Ann. des Naturhistorischen Museums Wien. Ser. A, Fur Mineral. und Petrogr. Geol. und Palaontologie, Anthropol. und Prahistorie 117, 121 (2015).
Jagt, J. W. M., Bakel, B. W. M. Van & Artal, P. Necrocarcinus ornatissimus forir, 1887, and Prehepatus werneri Fraaye & Collins, 1987 (Upper Maastrichtian, the Netherlands) revisited, with notes on other Cretaceous dynomenid crabs (Decapoda, Brachyura). Crustac. Monogr. 11, 173–195 (2010).
Spiridonov, V. A., Neretina, T. V. & Schepetov, D. Morphological characterization and molecular phylogeny of Portunoidea Rafinesque, 1815 (Crustacea Brachyura): Implications for understanding evolution of swimming capacity and revision of the family-level classification. Zool. Anzeiger-A J. Comp. Zool. 253, 404–429 (2014).
Manning, R. B. & Holthuis, L. B. West African brachyuran crabs. Sm. C. Zool. 306, 1–379 (1981).
Ng, P. K. L., Guinot, D., Davie, P. J. F., Systema Brachyurorum & Part, I. An annotated checklist of extant brachyuran crabs of the world. Raff. B. Zool. Suppl. 17, 1–286 (2008).
Lai, J. C. Y., Mendoza, J. C. E., Guinot, D., Clark, P. F. & Ng, P. K. L. Xanthidae MacLeay, 1838 (Decapoda: Brachyura: Xanthoidea) systematics: A multi-gene approach with support from adult and zoeal morphology. Zool. Anz. 250(4), 407–448 (2011).
Rathbun, M. J. Decapod crustaceans from the Upper Cretaceous of North Carolina. North Carolina Geol. Surv. 5, 403–408 (1923).
Rathbun, M. J. New species of South Dakota Cretaceous crabs. Proc. United States Natl. Museum 52, 385–391 (1917).
Kesling, R. V. & Reimann, I. G. An Upper Cretaceous crab, Avitelmessus grapsoideus Rathbun. Contrib. from museum Paleontol. Univ. Michigan 14, 1–15 (1957).
Van Straelen, V. Crustacés Décapodes nouveaux ou peu connus de l’époque Crétacique. Bull. du Musée R. d’Histoire Nat. Belgique 12, 1–50 (1936).
Guinot, D., Vega, F. J. & Van Bakel, B. W. M. Cenomanocarcinidae n. fam., a new Cretaceous podotreme family (Crustacea, Decapoda, Brachyura, Raninoidia), with comments on related families. Geodiversitas 30, 681–719 (2008).
Bosquet, J. A. H. Monographie des Crustaces fossiles du terrain Cretace du Duche de Limbourg. Verh. Uitg. door Comm. belast met het vervaardigen eener Geol. Beschrijv. en kaart van Ned. 2, 13–138 (1854).
Jagt, J. W. M., Fraaije, R. H. B. & van Bakel, B. W. M. Decapod crustacean’odds and ends’ from the Maastrichtian type area (southeast Netherlands, northeast Belgium) Distefania (?) vanrijsselti n. sp. Scr. Geol (2014).
Rathbun, M. J. Fossil Crustacea of the Atlantic and Gulf coastal plain. Geol. Soc. Am. Spec. Pap. 2, 1–160 (1935).
Karasawa, H. et al. Neogene and Quaternary ghost shrimps and crabs (Crustacea: Decapoda) from the Philippines. Bull. Natl. Museum Nat. Sci. 34, 51–76 (2008).
Maury, C. J. Cretaceo da Parahyba do Norte. Serviço Geol. e Mineral. do Bras. Monogr. 8, 1–350 (1930).
Ossó, À. Eogeryon elegius n. gen. and n. sp. (Decapoda: Eubrachyura: Portunoidea), one of the oldest modern crabs from late Cenomanian of the Iberian Peninsula (2016).
Vega, F. J. et al. Morphology and size variation of a portunoid crab from the Maastrichtian of the Americas. J. South Am. Earth Sci. 47, 116–135 (2013).
Ossó-Morales, À., Artal, P. & Vega, F. J. New crabs (Crustacea, Decapoda) from the Upper Cretaceous (Campanian) of the Moyenne Moulouya, northeast Morocco. Rev. Mex. Ciencias Geológicas 27, 213–224 (2010).
Dietl, G. P. & Vega, F. J. Specialized shell-breaking crab claws in Cretaceous seas. Biol. Lett. 4, 290–293 (2008).
Colosi, G. Una specie fossile de Gerionide (Decapodi brachiuri). Bolettino della Soc. dei Nat. Napoli 35, 248–255 (1923).
Secretan, S. Une nouvelle espèce de xanthidés au Maroc: Titanocarcinus meridionalis nov. sp. Notes Serv. Géologique Maroc 20, 39–50 (1961).
Van Straelen, V. Description de Brachyoures montiens du Cominges. Bull. la Soc. Belgique Geol. 34, 58–62 (1924).
Vega, F. J. et al. Maastrichtian Crustacea (Brachyura: Decapoda) from the Ocozocuautla Formation in Chiapas, southeast México. J. Paleontol. 75, 319–329 (2001).
Matheron, P. Notice sur les reptiles fossiles des dépôts fluvio-lacustres crétacés du bassin à lignite de Fuveau. (F. Savy, 1869).
De Lapparent De Broin, F. & Murelaga, X. Une nouvelle faune de chéloniens dans le Crétacé supérieur européen. Comptes rendus de l'Académie des sciences. Série 2. Sciences de la terre et des planètes 323(8), 729–735 (1996).
Portis, A. Les chéloniens de la molasse vaudoise observé dans le Musée géologique de Lausanne. (Schuchardt, 1882).
Nopcsa, F. Paleontological notes on Reptilia. 7. Classification of the Crocodilia. Geol. Hungarica, Ser. Palaeontol. 1, 75–84 (1928).
Delfino, M. et al. A complete skull of Allodaposuchus precedens Nopcsa, 1928 (Eusuchia) and a reassessment of the morphology of the taxon based on the Romanian remains. J. Vertebr. Paleontol. 28, 111–122 (2008).
Puértolas-Pascual, E., Canudo, J. I. & Moreno-Azanza, M. The eusuchian crocodylomorph Allodaposuchus subjuniperus sp. nov., a new species from the latest Cretaceous (upper Maastrichtian) of Spain. Hist. Biol. 26, 91–109 (2014).
Blanco, A. et al. A new species of Allodaposuchus (Eusuchia, Crocodylia) from the Maastrichtian (Late Cretaceous) of Spain: phylogenetic and paleobiological implications. PeerJ 3, e1171 (2015).
Blanco, A., Puértolas-Pascual, E., Marmi, J., Vila, B. & Sellés, A. G. Allodaposuchus palustris sp. nov. from the Upper Cretaceous of Fumanya (South-Eastern Pyrenees, Iberian Peninsula): systematics, palaeoecology and palaeobiogeography of the enigmatic allodaposuchian crocodylians. PLoS One 9, e115837 (2014).
Taplin, L. E., Grigg, G. C., Harlow, P., Ellis, T. M. & Dunson, W. A. Lingual salt glands in Crocodylus acutus and C. johnstoni and their absence from Alligator mississipiensis and Caiman crocodilus. J. Comp. Physiol. B Biochem. Syst. Environ. Physiol. 149, 43–47 (1982).
Pérez-García, A. The Iberian fossil record of turtles: an update. J. Iber. Geol. 43, 155–191 (2017).
Tong, H., Gaffney, E. S. & Buffetaut, E. Foxemys, a new side-necked turtle (Bothremydidae, Pelomedusoides) from the late Cretaceous of France. American Museum novitates; no. 3251. (1998).
Gaffney, E. S., Tong, H. & Meylan, P. A. Evolution of the side-necked turtles: the families Bothremydidae, Euraxemydidae, and Araripemydidae. Bull. Am. Museum Nat. Hist. 2006, 1–699 (2006).
Pérez-García, A. & Ortega, F. Identification of the French Upper Cretaceous bothremydid turtle Foxemys mechinorum in the Spanish record. Geobios 51, 211–217 (2018).
Joyce, W. G., Chapman, S. D., Moody, R. T. J. & Walker, C. A. The skull of the solemydid turtle Helochelydra nopcsai from the Early Cretaceous of the Isle of Wight (UK) and a review of Solemydidae. Spec. Pap. Palaeontol. 86, 75–97 (2011).
Case, G. & Cappetta, H. Additions to the elasmobranch fauna from the Upper Cretaceous of New Jersey (Middle Maastrichtian, Navesink Formation). Palaeovertebrata 33, 1–16 (2004).
Cuny, G., Suteethorn, V. & Buffetaut, E. Freshwater hybodont sharks from the Lower Cretaceous of Thailand. Biol. Conserv. Freshw. Elasmobranchs, Am. Fish. Soc. Bethesda, Maryl. 15–26 (2004).
Cavin, L., Valentin, X. & Garcia, G. A new mawsoniid coelacanth (Actinistia) from the Upper Cretaceous of Southern France. Cretac. Res. 62, 65–73 (2016).
Webb, G. E. & Kamber, B. S. Rare earth elements in Holocene reefal microbialites: a new shallow seawater proxy. Geochim. Cosmochim. Acta 64, 1557–1565 (2000).
Nothdurft, L. D., Webb, G. E. & Kamber, B. S. Rare earth element geochemistry of Late Devonian reefal carbonates, Canning Basin, Western Australia: confirmation of a seawater REE proxy in ancient limestones. Geochim. Cosmochim. Acta 68, 263–283 (2004).
Mavotchy, N. O. et al. The role of the early diagenetic dolomitic concretions in the preservation of the 2.1-Ga paleoenvironmental signal: The Paleoproterozoic of the Franceville Basin, Gabon. Comptes Rendus Géoscience 348, 609–618 (2016).
Høgdhal, O., Melsom, S. & Bowen, V. In Trace Inorganics In Water 308–325 (ACS Publications, 1968).
Bolhar, R., Kamber, B. S., Moorbath, S., Fedo, C. M. & Whitehouse, M. J. Characterisation of early Archaean chemical sediments by trace element signatures. Earth Planet. Sci. Lett. 222, 43–60 (2004).
Nozaki, Y., Lerche, D., Alibo, D. S. & Tsutsumi, M. Dissolved indium and rare earth elements in three Japanese rivers and Tokyo Bay: evidence for anthropogenic Gd and. Geochim. Cosmochim. Acta 64, 3975–3982 (2000).
Locatelli, E. R. Experimental taphonomy of decapod crustaceans: assessing the effects of pre-burial processes. In Geological Society of America Annual Meeting. Abstracts with programm 102–137 (2012).
Klompmaker, A. A., Portell, R. W. & Frick, M. G. Comparative experimental taphonomy of eight marine arthropods indicates distinct differences in preservation potential. Palaeontology 60, 773–794 (2017).
Gallagher, W. B., Parris, D. C. & Spamer, E. E. Paleontology, biostratigraphy, and depositional environments of the Cretaceous-Tertiary transition in the New Jersey Coastal Plain. The Mosasaur 3, 1–35 (1986).
Gallagher, W. B. The Cretaceous/Tertiary mass extinction event in the northern Atlantic Coastal Plain. The Mosasaur 5, 75–154 (1993).
Kuehne, D. Further examination of the Woodbury and Englishtown Formations in Camden County and adjacent areas, New Jersey. The Mosasaur 5, 63–72 (1993).
Schweitzer, C. E. et al. Mangrove-dwelling crabs (Decapoda: Brachyura: Necrocarcinidae) associated with dinosaurs from the Upper Cretaceous (Cenomanian) of Egypt. J. Paleontol. 77, 888–894 (2003).
Bott, R. Die Süßwasserkrabben von Afrika (Crust., Decap.) und ihre Stammesgeschichte. Ann. du Mus. R. du Congo Belge - Ser. C 1, 213–349 (1955).
Schubart, C. D. & Ng, P. K. L. A new molluscivore crab from Lake Poso confirms multiple colonization of ancient lakes in Sulawesi by freshwater crabs (Decapoda: Brachyura). Zool. J. Linn. Soc. 154, 211–221 (2008).
Chin, K., Feldmann, R. M. & Tashman, J. N. Consumption of crustaceans by megaherbivorous dinosaurs: Dietary flexibility and dinosaur life history strategies. Sci. Rep. 7, 1–11 (2017).
Kirkland, J. I. An inventory of paleontological resources in the lower Wahweap Formation (lower Campanian), Southern Kaiparowits plateau, Grand staircase ľ Escalante National monument, Utah. In 2005 Salt Lake City Annual Meeting (2005).
DeBlieux, D. D. et al. Paleontological overview and taphonomy of the middle Campanian Wahweap Formation in Grand Staircase-Escalante National Monument. Top Gd. Staircase Late Cretac. South. Utah 563–587 (2013).
de Broin, F. et al. Nouvelles découvertes de vertébrés maestrichtiens dans le gisement de Fox-Amphoux (Var). 8e Réunion Annu. des Sci. la Terre, Marseille 68 (1980).
Codrea, V., Solomon, A., Fărcaș, C. & Barbu, O. On some local restricted Maastrichtian environments of the “Hațeg Island” (Transylvania, Romania). Bull. Geol. Soc. Greece 47, 82–91 (2013).
De Grave, S., Cai, Y. & Anker, A. Global diversity of shrimps (Crustacea: Decapoda: Caridea) in freshwater. Hydrobiologia 595, 287–293 (2008).
Kemp, S. Notes on Crustacea Decapoda in the Indian Museum XVII. On various Caridae. Rec. Indian. Museum 27, 249–343 (1925).
Abele, L. G. Semi-terrestrial shrimp (Merguia rhizophorae). Nature 226, 661 (1970).
Crandall, K. A. & Buhay, J. E. In Freshwater animal diversity assessment 295–301 (Springer, 2007).
Holmes, S. J. On some new or imperfectly known species of west American Crustacea. Proc. Calif. Acad. Sci. (3, Zool. 3, 307–331 (1904).
Dworschak, P. C. First record of Lepidophthalmus tridentatus (von Martens, 1868)(Callianassidae) from the Philippines. Ann. des Naturhistorischen Museums Wien. Ser. B für Bot. und Zool. 121–130 (2006).
White, A. Descriptions of two species of Crustacea belonging to the families Callianassidae and Squillidae. In Proceedings of the Scientific Meetings of the Zoological Society of London 42–44 (1861).
Vanhöffen, E. Über die Krabben, denen Kamerun seinen Namen verdankt. Sitzungsberichte der Gesellschaft Naturforschender Freunde zu Berlin 1911, 105–110 (1911).
Dana, J. D. Conspectus crustaceorum, quae in orbis terrarum circumnavigatione, Carolo Wilkes, e classe Reipublicae foederatae duce, lexit et descripsit Jacobus D. Dana. Pars II. Proc. Am. Acad. Arts Sci. 2nd Ser. 2, 6–61 (1852).
Bond-Buckup, G. & Buckup, L. A família Aeglidae (Crustacea, Decapoda, Anomura). Arq. Zool. 32, 159–346 (1994).
Perez-Losada, M., Jara, C. G., Bond-Buckup, G., Porter, M. L. & Crandall, K. A. Phylogenetic position of the freshwater anomuran family Aeglidae. J. Crustac. Biol. 22, 670–676 (2002).
Pérez-Losada, M., Bond-Buckup, G., Jara, C. G. & Crandall, K. A. Molecular systematics and biogeography of the southern South American freshwater “crabs” Aegla (Decapoda: Anomura: Aeglidae) using multiple heuristic tree search approaches. Syst. Biol. 53, 767–780 (2004).
Cumberlidge, N. et al. Freshwater crabs and the biodiversity crisis: importance, threats, status, and conservation challenges. Biol. Conserv. 142, 1665–1673 (2009).
Haan de, W. In Fauna japonica, sive, Descriptio animalium, quae in itinere per Japoniam, jussu et auspiciis, superiorum, qui summum in India Batava imperium tenent, suscepto, annis 1823-1830. Volume 1: Crustacea. (ed. Siebold, P. F. V.; Haan, W. D.; Schlegel, H. & Temminck, C. J.) 243 (1833).
Milne Edwards, H. In Dictionnaire Classique d’Histoire Naturelle (ed. Bory de Saint-Vincent, J.-B. G. M.) 16 (Ray et Gravier; Amable Gobin et Cie, 1830).
Ng, P. K. L. The freshwater crabs of Peninsular Malaysia and Singapore (1988).
Ng, P. K. L. In Freshwater invertebrates of the Malaysian region (eds. Yong, H. S. & Yule, C. M.) 311–336 (Akademi Sains Malaysia, 2004).
Davie, P. J. F. & Ng, P. K. L. A new genus for cave-dwelling crabs previously assigned to Sesarmoides (Crustacea: Decapoda: Brachyura: Sesarmidae). Raffles Bull. Zool. Suppl. 16, 227–231 (2007).
De Man, J. G. In Zoologische Ergebnisse einer Reise in Niederlandisch OstIndien (ed. Weber, M.) 165–527 (1892).
Feldmann, R. M., Grande, L., Birkhimer, C. P., Hannibal, J. T. & McCoy, D. L. Decapod fauna of the Green River formation (Eocene) of Wyoming. J. Paleontol. 788–799 (1981).
Hasiotis, S. T. & Mitchell, C. E. A comparison of crayfish burrow morphologies: Triassic and Holocene fossil, paleo‐and neo‐ichnological evidence, and the identification of their burrowing signatures. Ichnos An Int. J. Plant Anim. 2, 291–314 (1993).
Rabadà i Vives, D. Crustáceos decápodos lacustres de las calizas litográficas del Cretácico inferior de España: Las Hoyas (Cuenca) y el Montsec de Rúbies (Lleida) (1993).
Taylor, R. S., Schram, F. R. & Shen, Y.-B. A new crayfish family (Decapoda: Astacida) from the Upper Jurassic of China, with a reinterpretation of other Chinese crayfish taxa. Paleontol. Res. 3, 121–136 (1999).
Martin, A. J. et al. Fossil evidence in Australia for oldest known freshwater crayfish of Gondwana. Gondwana Res. 14, 287–296 (2008).
Beurlen, K. Alguns restos de crustáceos decápodes d’água doce fósseis no Brasil. An. da Acad. Bras. ciencias 22, 453–459 (1950).
Houša, V. Bechleja inopinata ng, n. sp. ein neuer Krebs aus dem bohmischen Tertiar (Decapoda, Palaemonidae). Ustred Ust. Geol. 23, 365–377 (1956).
Martins-Neto, R. G. & Mezzalira, S. Revisão dos Palemonídeos Terciários Brasileiros (Crustacea, Caridea) com descrição de novos taxa. An. Acad. Bras. Cienc. 63, 361–367 (1991).
Garassino, A., Yanbin, S., Schram, F. R. & Taylor, R. S. Yongjicaris zhejiangensis n. gen. n. sp.(Crustacea, Decapoda, Caridea) from the Lower Cretaceous of Zhejiang Province, China. Bull. Mizunami Foss. Museum 29, 73–80 (2002).
Feldmann, R. M. Haumuriaegla glaessneri n. gen. and sp.(Decapoda; Anomura; Aeglidae) from Haumurian (late Cretaceous) rocks near Cheviot, New Zealand. New Zeal. J. Geol. Geophys. 27, 379–385 (1984).
De Angeli, A. & Caporiondo, F. Alontecarcinus buratoi n. gen., n. sp. (Decapoda, Brachyura, Potamonidae) un nuovo crostaceo dacqua dolce dellEocene (Bartoniano) di Alonte (Monti Berici, Vicenza, Italia settentrionale). Bolletino del Mus. di Stor. Nat. di Verona in press.
Ma, K. Y. et al. Phylogenomic analyses of brachyuran crabs support early divergence of primary freshwater crabs. Mol. Phylogenet. Evol. 135, 62–66 (2019).
Garassino, A., Pasini, G. & Dutheil, D. B. Cretapenaeus berberus n. gen., n. sp. (Crustacea, Decapoda, Penaeidae) from the Late Cretaceous (Cenomanian) of southeastern Morocco. Atti della Soc. Ital. di Sci. Nat. e del Mus. Civ. di Stor. Nat. di Milano 147, 3–17 (2006).
Breinholt, J., Pérez-Losada, M. & Crandall, K. A. The timing of the diversification of the freshwater crayfishes. Decapod Crustac. phylogenetics 343–356 (2009).
Peach, B. N. Monograph on the higher Crustacea of the Carboniferous rocks of Scotland. Mem. Geol. Surv. Gt. Britain, Palaeontol. 1–82 (1908).
Gueriau, P., Charbonnier, S. & Clément, G. First decapod crustaceans in a Late Devonian continental ecosystem. Palaeontology 57, 1203–1213 (2014).
Jones, W. T., Feldmann, R. M., Schram, F. R., Schweitzer, C. E. & Maguire, E. P. The proof is in the pouch: Tealliocaris is a peracarid. Palaeodiversity 9, 75–89 (2016).
Bate, C. S. On a new genus, with four new species of freshwater prawns. Proc. Zool. Soc. London 1868, 363–368 (1868).
Botello, A. & Alvarez, F. Phylogenetic relationships among the freshwater genera of palaemonid shrimps (Crustacea: Decapoda) from Mexico: evidence of multiple invasions? Lat. Am. J. Aquat. Res. 41, 773–780 (2013).
Crandal, K. A., Harris, D. J. & Fetzner, J. W. Jr. The monophyletic origin of freshwater crayfish estimated from nuclear and mitochondrial DNA sequences. Proc. R. Soc. London. Ser. B Biol. Sci. 267, 1679–1686 (2000).
Klaus, S., Schubart, C. D. & Brandis, D. Phylogeny, biogeography and a new taxonomy for the Gecarcinucoidea Rathbun, 1904 (Decapoda: Brachyura). Org. Divers. Evol. 6, 199–217 (2006).
Sternberg von, R. & Cumberlidge, N. In Advances in Decapod Crustacean Research 21–39 (Springer, 2001).
Daniels, S. R., Cumberlidge, N., Pérez-Losada, M., Marijnissen, S. A. E. & Crandall, K. A. Evolution of Afrotropical freshwater crab lineages obscured by morphological convergence. Mol. Phylogenet. Evol. 40, 227–235 (2006).
Le Loeuff, J. Romanian Late Cretaceous dinosaurs: big dwarfs or small giants? Hist. Biol. 17, 15–17 (2005).
Authors want to acknowledge Clément Jauvion for discussions on elementary ratio in marine/non marine sediments. X.V. and G.G. thank Y. Dutour and E. Turini for photographs of the specimen from Rognac. G.G. and X.V. gratefully acknowledge the management, logistical, and communication assistance from the Velaux Municipality (J.-P. Maggi and L. Melhi) with its heritage, culture, and technical services (M. Calvier and S. Chauvet), the environment department from CD 13 (M. Bourrelly, T. Tortosa, G. Michel, N. Mouly, and S. Amico), the Service Départemental d'Incendie et de Secours (SDIS) 13, and numerous volunteers during the field campaigns in 2009 and 2012. This work was supported in part by the French Ministry of Culture and Communication (research grant VR1013 to the Palaios Association) and the Bouches-du-Rhône department (CD 13) (proposals MAPADGAC23112010-1 and MAPADGAC16012014-1-AAPC). We wish to thank Sean Murphy for English review of this work. Authors finally thank S. Klaus, R. Feldmann, and an anonymous reviewer for comments on manuscript improvements and discussion on claws systematic assignement.
The authors declare no competing interests.
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Robin, N., van Bakel, B.W.M., Hyžný, M. et al. The oldest freshwater crabs: claws on dinosaur bones. Sci Rep 9, 20220 (2019). https://doi.org/10.1038/s41598-019-56180-w