CELVEC as a drug delivery vehicle. (A) Loading of doxorubicin (DOX) into CELVEC as a function of initial DOX concentration used. (B) Release of DOX from macrophages over 24 h when loading was performed passively (left) or supplemented with electroporation (right). Inset graphs depict DOX release over 6 h as determined using a one-phase association ordinary fit line. Dotted black line designated 50% release of payload. (C) Cellular viability of human MDA-231 triple negative breast cancer cells following treatment with free DOX and DOX-loaded CELVEC at low (325 nM) and high (975 nM) dosages. The data is plotted as the mean ± SEM. *p < 0.05; **p < 0.01; ****p < 0.0001.