Step-by-step demonstration of the mounting of the diaphragm strip to the clamps and then to the muscle force system. (A,B) Mounting the central tendon to the tendon clamp. Asterisk, the hex wrench used to secure the clamp plates with the nylon hex screws. (C–E) Mounting the rib to the rib clamp and assembling the rib clamp. White arrowhead, stainless steel hook. Black arrow, a gap between the top plate and the bottom plate of the rib clamp. This is intentionally designed so that the rib clamp will not clamp on the diaphragm muscle after the clamp is assembled. (F) The final assembly of the diaphragm strip to the muscle force assay system using the clamp approach. White arrows, platinum electrodes. White arrowhead, stainless steel hook.