Allergen exposure enhances synchrony and inverses directionality of theta activity in mPFC-amygdala circuit. (A,D) Examples of mPFC and amygdala delta (<4 Hz) and theta (4–12 Hz) filtered signals, illustrating the cross-correlation lag analysis in the control (upper) and OVA (lower). Arrows are drawn from the leading area to the lagging area. (B,E) Mean waveform correlation for the delta and theta-filtered signals in the mPFC-amygdala circuit. Shaded area indicates standard errors. (C,F) Mean mPFC-amygdala correlation and lag at delta (<4 Hz) and theta (4–12 Hz) frequencies. OVA exposure increases correlation for theta oscillation between mPFC and amygdala with a delay in the mPFC. The horizontal bars represent mean values. Data were analyzed by t-test, n = 8 per group. *p < 0.05, ***p < 0.001 compared to control. OVA, ovalbumin; mPFC, medical prefrontal cortex; LFP, local field potential.