Allergen exposure induces anxiety-like behavior. (A) Representative animal track in the elevated zero maze for a control (upper panels) and an OVA (lower panels) rat. (B,C) Time spent in open sections and number of entries to the open sections in the elevated zero maze, which inversely related to anxiety-like behavior respectively (Control: n = 14, OVA: n = 11). (D) Total distance traveled (as an indicator of locomotor activity) in the open field test (Control: n = 13, OVA: n = 12). The horizontal bars represent mean values. Data were analyzed by t-test. **p < 0.01, ***p < 0.001 compared to control. ns, not significant; OVA, ovalbumin.