Table 2 Chromatography methods used for quantification of paracetamol, diazepam and insulin. TFA: trifluoroacetic acid.

From: Impact of alternative materials to plasticized PVC infusion tubings on drug sorption and plasticizer release

APIChromatography columnMobile PhaseModeFlow rate
(mL/min)
Oven Temperature (°C)Detection wavelength (nm)Injection volume (µL)
ParacetamolGemini C18 3 µm,
150 × 4,6 mm
Precolumn Gemini C18
5 µm, 0,04 × 0,03 cm
(Phenomenex, France)
Phase A:
Water adjusted to pH 2.75 with formic acid 98%
Phase B:
Acetonitrile
Gradient (% Phase A):
0–3 min: 90%
3–7 min: 90% to 40%
7–10 min: 40%
1.34024310
DiazepamNucleodur C18 HTEC 5 µm, 125 × 4,6 mm (Macherey-Nagel, France)v/v/v
22% Acetonitrile
34% Methanol
44% phosphate buffer (3.4 g/L, pH = 5.0)
isocratic1.03025420
InsulinNucleodur C18 ec 5 µm, 250 × 4,6 mm
(Macherey Nagel, France)
Phase A:
TFA/water 0,1% (v/v)
Phase B:
TFA/acetonitrile 0,08% (v/v)
Gradient (% Phase A):
0–15 min: 80% to 35%
15–17 min: 35%
17–20 min: 35% to 80%
1.32528020