Ikbkap-deficient oocytes display impaired microtubule–kinetochore attachment. (A) Ctrl and CKO MII oocytes were immunostained for acetylated α-tubulin (green), CREST (red), and DAPI (blue). Representative confocal images are shown. Right panels show enlarged view of boxed areas in middle panels. Arrows indicate bioriented and attached kinetochores; arrowheads show defective and unattached kinetochores. Scale bars, 5 µm. (B) Quantification of Ctrl and CKO oocytes with impaired microtubule-kinetochore attachment. Data are expressed as mean ± SEM of results obtained from at least 3 independent experiments in which ≥45 oocytes for each group were analyzed. ***p < 0.001.