Figure 4 | Scientific Reports

Figure 4

From: Early antibody response and clinical outcome in experimental canine leishmaniasis

Figure 4

(A) 2D electrophoretic separation of soluble Leishmania antigens (SLA). (B) Western blot of SLA with serum (1/50) from a dog with a chronic L. infantum infection. Two 2D gels were run in parallel: the first one (A) was stained with Coomassie blue; the second one was transferred to a PVDF membrane for Western blot (B). The strip on the left was used as transfer control of SLA and markers. MW: molecular weight markers in KDa; pH 3–11: pH gradient. Circles: selected spots for peptide identification by mass spectrometry and finger printing.

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