Figure 3 | Scientific Reports

Figure 3

From: Essential role of the initial activation signal in isotype selection upon deletion of a transcriptionally committed promoter

Figure 3

CSR to IgG2b is partially restored in response to TGF-β but not to LPS stimulation. (A,B) CH12 cells, three Iα-deleted clones (2, 3, and 5) and splenic B cells were activated by LPS (A) or LIT (B) for 4 days, and stained for IgG2b. Representative plots are shown for unstimulated (UNS), activated CH12 cells, Iα-deleted clones (clone 5) and primary B cells. (C,D) RT-qPCR quantification of Sγ2b pre-switch transcripts levels in unstimulated clones 5, 6 and 8 and in response to LPS (C) or to LIT (D) (day 2). (E) Comparison of Sγ2b pre-switch transcripts levels in Iα-deleted clones 5, 6 and 8, following LPS and LIT stimulation (n = 3).

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