Figure 4 | Scientific Reports

Figure 4

From: Pseudomonas aeruginosa uses multiple receptors for adherence to laminin during infection of the respiratory tract and skin wounds

Figure 4

Pseudomonas aeruginosa receptors bind laminin with wide range of affinities. Recombinantly expressed proteins EstA, OprD, OprG, PA3923 and Paf, purified with affinity chromatography, were subjected to laminin-coated plates and binding was detected by ELISA using anti-His pAbs. In addition, Moraxella IgD-binding protein (MID), was added to the experiments as negative control. Three biological repetitions were done. The binding to laminin of each of the four outer membrane proteins and Paf were dose-dependent up to saturation (a). The interactions were further evaluated with biolayer interferometry (b–f) and dissociation constants (KD) calculated from the fitting model.

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