Each laminin-receptor contribute to the laminin-adhering phenotype. Pseudomonas aeruginosa mutants from a transposon library were examined for adherence to laminin-111 coated on glass slides (mean and standard error mean (SEM)). Bovine serum albumin (BSA) was used for negative control. P-values shown indicate the statistical difference of the means of adherent bacteria to laminin- versus to BSA-coated slides, as evaluated with repeated t-test and correction for multiple comparisons using the Holm-Sidak method. Adherent bacteria were automatically counted by the image analysis software cellSens in 6 randomly selected regions of interest, in biological triplicates. *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001. Representative images for wild type PAO1 and each mutated strain as denoted are shown in the supplemental data, Fig. S1.