Steady state inhibition induced by GP activity. (A) Average change in EP membrane potential during steady state inhibition induced by simulated regular stimulation (black) and experimental data (blue). The GP was stimulated at 5 Hz (n = 24), 10 Hz (n = 24), 20 Hz (n = 24), 30 Hz (n = 11), 40 Hz (n = 9), 60 Hz (n = 5) and 80 Hz (n = 6). (B) Distribution of the limiting frequency calculated for 9 individual GP-EP synapses, calculated from GP evoked IPSPs. (C,D) Example of calculation of the limiting frequency of a GP-EP synapse. (C) Change of the EP membrane potential as a function of the stimulation frequency of the GP (red dots). The data were fitted with an exponential function (blue trace). (D) The limiting frequency was extracted as the activation frequency that induced a change in Vm that was smaller than 5%.