Repression of Iα5 subunit during cardiac differentiation led to a downregulation of mesoderm and EMT markers in mesodermal progenitor cells. (A) (i) RT-qPCR analysis of the transcription factor TBX6 in MPC. Data were normalized to a control without dox-treatment. (ii) Histogram of flow cytometry analysis for TBX6 in dox-treated and control cells at day 4 of cardiac differentiation. Positive cells for TBX6 are represented as the mean ± SEM for three independent experiments. (B) RT-qPCR analysis of different genes involved in mesoderm and cardiac specification. Data were normalized to a control without dox-treatment (n = 3). (C) (i) Density plot showing both sorted cell populations and RT-qPCR analysis of Iα5 subunit mRNA expression. (ii) RT-qPCR analysis of different genes involved during EMT process. Results are presented as means ± SEM for four independent experiments and plotted in log2 scale. Data were normalized to CD56+/Iα5- cell population. *p < 0.05.