Co-encapsulation of EC and NSC Promotes NSC Quiescence and Enhances NSC Viability. NSC:EC seeding density depicting brightfield (top row) and GFP-tagged NSC (bottom row) at 4 different ratios (B) Quantification for cell mean fluorescence intensity for Ki67+ NSC 4 different ratios of NSC:EC determined through flow cytometry (n = 3). (C) Fluorescent images of NSC mono- and co-culture (left and right panel, respectively), stained for Sox2, Ki67, Hoechst, and merged. Imaged at 1, 3, and 7 days (top, middle, bottom panel, respectively) with quantified Sox2 + Ki67+ cells in E (n = 3). (D) Fluorescent images of NSC mono- and co-culture stained for cleaved caspase-3 with quantified Sox2+ Caspase3- cells in F (n = 3). Error bars represent SEM. Scale bar (50 µm) representative of all images. *p ≤ 0.05, ***p ≤ 0.001, ****p ≤ 0.0001 determined by an unpaired t test.