Effect of the damping parameter on noise amplification. (a) Single slice out of a light sheet microscopy image stack comprising 51 slices recorded from a GFP-expressing mouse brain. For demonstrating, the effect of the damping parameter, artificial computer generated Gaussian noise was added. (b) Deconvolution of the same data without damping causes an amplification of the noise, masking fine details of the image. The quality after deconvolution is even worse than the quality before deconvolution. (c) Deconvolution of the same data set using 5% damping. The amplification of noise is significantly reduced and a quality improvement compared to the raw data (A) is clearly recognizable now.