Liver histology and hepatic BSEP expression in PFIC2 patients (Patients 1 and 2) before and during the course of NaPB therapy. (a) Liver histology. The liver sections were subjected to HE, Azan, or Masson’s trichrome staining. A typical image under each condition is shown. Original magnification; 400× (HE staining) and 100× (Azan and Masson’s trichrome staining). Bar, 100 μm. (b,c) Hepatic BSEP expression. The prepared membrane fractions (5 μg) were analyzed with those from age-matched control subjects through immunoblotting. The signal intensity of BSEP relative to that of ATP1A1 is presented below the panel. CHF, congenital hepatic fibrosis; HBV, hepatitis B virus; HCV, hepatitis C virus.