SMN complex member Gemin3 self-interacts and has a functional relationship with ALS-linked proteins TDP-43, FUS and Sod1

The predominant motor neuron disease in infants and adults is spinal muscular atrophy (SMA) and amyotrophic lateral sclerosis (ALS), respectively. SMA is caused by insufficient levels of the Survival Motor Neuron (SMN) protein, which operates as part of the multiprotein SMN complex that includes the DEAD-box RNA helicase Gemin3/DDX20/DP103. C9orf72, SOD1, TDP-43 and FUS are ranked as the four major genes causing familial ALS. Accumulating evidence has revealed a surprising molecular overlap between SMA and ALS. Here, we ask the question of whether Drosophila can also be exploited to study shared pathogenic pathways. Focusing on motor behaviour, muscle mass and survival, we show that disruption of either TBPH/TDP-43 or Caz/FUS enhance defects associated with Gemin3 loss-of-function. Gemin3-associated neuromuscular junction overgrowth was however suppressed. Sod1 depletion had a modifying effect in late adulthood. We also show that Gemin3 self-interacts and Gem3ΔN, a helicase domain deletion mutant, retains the ability to interact with its wild-type counterpart. Importantly, mutant:wild-type dimers are favoured more than wild-type:wild-type dimers. In addition to reinforcing the link between SMA and ALS, further exploration of mechanistic overlaps is now possible in a genetically tractable model organism. Notably, Gemin3 can be elevated to a candidate for modifying motor neuron degeneration.


Results overexpression of human TDP-43 in a Gem3 mutant background induces adult lethality.
Similar to SMN 45,62,63 , loss of Gemin3 impacts adult viability and induces motor dysfunction [64][65][66] . In addition to Gemin3, a select number of SMN complex components, including Gemin2, Gemin4, Gemin5, Gemin8 and Unrip, are required for neuromuscular function and survival in Drosophila 61,64,67 . It is therefore highly plausible that SMA is triggered by any perturbation in the stoichiometry of the SMN complex. We have recently isolated Gem3 BART , a hypomorphic version of the Gem3 ΔN mutant, which lacks the N-terminal helicase core. Subsequently, we reported that alterations in the levels of SMN complex components precipitate the viability and motor phenotypes of Gem3 BART adult flies 68 . A similar outcome was observed on disruption of snRNP biogenesis factors pICln and Tgs1 61 . We wished to investigate whether a functional interaction also extends to Gemin3 and ALS-linked TDP-43. Missense mutations in this protein have been identified in 5% of familial and <1% of sporadic ALS cases 10 . TDP-43, an evolutionarily conserved protein, comprises of 2 RNA recognition motifs (RRMs), a nuclear localisation signal and a nuclear export sequence that mediate nuclear shuttling, as well as a C-terminal glycine-rich region where the majority of ALS-associated mutations occur 11 . Importantly, truncated TDP-43 is mislocalised from its predominantly nuclear location to ubiquitin-containing cytoplasmic inclusions in neurons of both sporadic and most familial forms of ALS 10,69,70 . Loss of TDP-43 nuclear function has been proposed as a primary mechanism linking TDP-43 proteinopathy to neuromuscular degeneration in ALS. In this context, we first asked whether decreased levels of TDP-43 can modify Gem3 BART phenotypes. We note that neither haploinsufficiency (TBPH Δ23 ) nor RNAi-induced knockdown (TBPH-RNAi) of TBPH, the Drosophila TDP-43 homologue, had any effect on motor and viability phenotypes in flies with muscle-restricted Gem3 BART expression (Fig. 1, Table 1 and data not shown).
Recently, considerable attention has been given to the toxic effect of cytoplasmic TDP-43 protein aggregates 71 . To this end, we next queried whether gain rather than loss of TDP-43 function is a modifying factor. Expression of wild-type human TDP-43 (hTDP-43 WT ) in muscle leads to adult flies that have climbing defects and are entirely flightless when compared to controls (Fig. 1). Flies also have a shortened life-span, therefore surviving less than a week post-eclosion. Notably, in combination with Gem3 BART , hTDP-43 induced adult lethality with flies dying at the pupal stage. Furthermore, transgenes with higher expression levels and/or epitope-tagged versions of hTDP-43 (hTDP-43 WT .GFP #10 , weak expression; hTDP-43 WT .GFP #16 , strong expression; ref. 72 ) were found to induce death at the earlier third instar stage (L3) when combined with Gem3 BART in contrast to death at pupal stage when they were expressed alone (Table 1). This trend, which was also observed with an independently-generated line (Flag.hTDP-43 WT , ref. 73 ; Table 1), shows that phenotypic enhancement is dependent on dose or modifications that interfere with protein structure. Muscle-directed expression of hTDP-43 CTF , which mimics the major TDP-43 C-terminal fragment found in cytosolic aggregates of ALS patients, was found to induce lethality at the first instar larval stage most likely because the transgene is highly expressing 72 . This excluded its use for interaction analysis (Table 1). Importantly, overexpression of endogenous TBPH replicated the modifier effect of its human counterpart. Hence, whereas alone it induces death at the pupal stage, when combined with Gem3 BART it enhanced survival defects with flies dying earlier than the L3 stage (Table 1). Overall, these findings are suggestive of a genetic interaction between Gemin3 and TBPH or its human homologue, TDP- 43. Knockdown or overexpression of caz/FUS enhances Gem3 mutant phenotypes. FUS is another major RBP that is mutated in both familial (5%) and sporadic (<1%) ALS cases 10 . FUS is a highly-conserved protein possessing an N-terminal domain rich in glutamine, glycine, serine and tyrosine residues (QGSY region), a glycine-rich region, an RRM, multiple arginine/glycine/glycine (RGG) repeats in an arginine-and glycine-rich region, and a zinc finger motif at the C-terminus. Mutations cluster in the glycine-rich region and in the extreme C-terminus where the nuclear localisation signal is likely to reside 11 . Similar to TDP-43, mutant FUS is mislocalised to the cytoplasm where it forms ubiquitinated aggregates 74 . Considering that FUS and TDP-43 function Flight performance was assessed by the Droso-Drome apparatus, where determination of flight capacity is based on which sector flies land after they are introduced at the top. Right: Removal of one copy (+/TBPH Δ23 ) or RNAi-mediated knockdown (TBPH-RNAi [Trinity]) of TBPH does not impair the motoric ability of flies with pan-muscular expression of Gem3 BART . Indeed, at all time points, no significant differences were observed between the two groups in the number of flies that had no flight ability (sector 1) compared to those that retained the ability to fly (sectors 2-4). In contrast, ectopic expression of wild-type human TDP-43 (hTDP-43 WT ) in muscle impairs flight as early as day 5 post-eclosion. Flies were non-fliers, hence they all fell in sector 1. (B) Climbing success rate of flies with muscle-specific hTDP-43 overexpression was drastically reduced compared to control animals. Furthermore, assessment of the time taken for the first fly to reach a pre-set threshold determined that flies took significantly longer to attain this goal in contrast to the control genotype. In (A,B), data presented are the mean ± S.E.M. of at least 4 independent experiments, and for each time point measured, n ≥ 60 per genotype. Symbols indicate the sex of the genotype assessed: ♂ = males, ♀ = females, and ♀♂ = males + females. Significance as tested by two-way ANOVA, followed by Bonferroni's post hoc tests (A) and the unpaired t-test (B) is indicated by the exact p-value.
www.nature.com/scientificreports www.nature.com/scientificreports/ in a common pathway with FUS acting downstream of TDP-43 75,76 , we hypothesised that Gemin3 is likely to have a functional relationship not only with TBPH/TDP-43 but also with FUS. To this end, we first tested whether haploinsufficiency of cabeza (caz), the Drosophila homologue of FUS, can induce motor deficits when placed in a Gem3 BART genetic background. Interestingly, we find a subtle yet statistically significant difference in motoric abilities at late adulthood (day 35 post-eclosion) in caz mutant heterozygous flies (caz 1 /+) that had muscle-restricted Gem3 BART expression compared to those that did not (Fig. 2). Subsequently, we asked whether phenotypic enhancement is dose-dependent. Hence, we induced muscle-specific RNAi-mediated knockdown of caz in wild-type versus Gem3 BART flies. We observed that a moderately-expressing RNAi transgene targeting the C-terminus (caz-RNAi [Kellogg], Supplementary Fig. S1) induced flight defects as early as day 15 post-eclosion with flies then exhibiting an age-dependent progressive worsening in phenotype (Fig. 2). A stronger RNAi transgene targeting the same region but based on short hairpin microRNA (shRNA) technology (caz-RNAi [Oriel], Supplementary Fig. S1) was capable of inducing motor defects at an earlier stage during adulthood (Fig. 2), further confirming that modification is dependent on Caz protein levels with a severe reduction inducing the highest impact.
Similar to TDP-43, in addition to loss of nuclear function, a toxic gain of function due to the formation of cytoplasmic aggregates has been implicated as a predominant mechanism underpinning FUS-associated pathophysiology 74,77 . In this context, we questioned whether upregulation of caz or overexpression of human FUS (hFUS WT ) can also act as enhancers when placed in a Gem3 mutant background. In this regard, in a wild-type background, muscle-restricted increase in Caz protein levels was sufficient to induce both climbing and flight defects as early as day 5 post-eclosion (Fig. 3). However, in Gem3 BART flies, caz WT upregulation induced lethality before eclosion with the majority of flies dying during the pupal stage (Table 1). Similarly, ectopic expression of hFUS WT in muscles was enough to cause motor deficits in young adult flies when applied to a wild-type background (Fig. 3). In combination with Gem3 BART , hFUS WT overexpression remarkably induced death during early development (Table 1). A trend towards reduced survival was also observed in Gem3 BART flies upon expression of hFUS with a pathogenic mutation in the C-terminus (hFUS P525L ), though not when expressing caz P398L , its equivalent in Drosophila (Table 1). Collectively, these findings show that either loss or gain of Caz/FUS function aggravate the motor and viability phenotypes of Gem3 mutant flies, which is highly suggestive of a genetic association between Gemin3 and caz/FUS. expression of C9orf72 repeat expansions has no effect on Gem3 mutant flies. We next sought to broaden our investigation by determining whether Gem3 mutant phenotypes are also induced by disruption www.nature.com/scientificreports www.nature.com/scientificreports/ of other major ALS-linked genes. C9orf72 is the most frequently mutated gene in ALS. Enormous expansions of an intronic hexanucleotide repeat (GGGGCC, G 4 C 2 ) cause a large portion of familial (25%) and sporadic (10%) ALS 10 . Transcripts containing repeats form intranuclear RNA foci that sequester nuclear proteins. In the cytoplasm, expanded RNA also undergoes repeat-associated non-AUG (RAN) translation to produce toxic dipeptide repeat (DPR) proteins 9 . Transgenic expression of a non-pathogenic repeat length (G 4 C 2 -3) in muscle tissue had no effect on neuromuscular function in either wild-type or Gem3 BART flies. Thus, at all time points assessed, flies were relatively healthy and no major differences were apparent between the two groups at any stage during their adult life (Fig. S2). Although muscle-restricted expression of 36 repeats (G 4 C 2 -36), previously shown to be neurotoxic 78 , caused flight defects in early adulthood, the phenotype in Gem3 BART flies was surprisingly identical to that of control flies with a wild-type background (Fig. S2). It is noteworthy that a similar outcome was observed in either background when assessing animals expressing two arginine-containing DPR proteins, glycine-arginine www.nature.com/scientificreports www.nature.com/scientificreports/ (GR-36) or proline-arginine (PR-100) (Fig. S2). In addition to confirming that repeats are damaging through the production of DPR proteins 78 , this result underscores that neither expanded repeats nor poly-GR/poly-PR proteins enhance Gem3 loss-of-function.
Loss rather than gain of Sod1 function enhances motor deficits in Gem3 mutant flies. The first ALS gene to be identified was SOD1, which encodes for the Cu-Zn superoxide dismutase, an abundant ubiquitously-expressed cytoplasmic enzyme. SOD1 is the second most commonly mutated gene in ALS, contributing to 20% and 2% of familial and sporadic cases, respectively 10 . SOD1 performs an important antioxidant function by catalysing the conversion of highly reactive superoxide to hydrogen peroxide or oxygen. Nevertheless, neuromuscular degeneration is thought to be driven by one or more acquired toxicities of the mutant protein rather than loss of dismutase activity. Indeed, similar to TDP-43 and FUS, most ALS-causing SOD1 mutants form ubiquitinated cytoplasmic aggregates that are toxic to various cellular processes 79 . Against this backdrop, we investigated whether gain of Sod1 function is also capable of triggering motor dysfunction in Gem3 mutant flies. We found that neither overexpression of Drosophila Sod1 nor ectopic expression of wild-type human SOD1 (hSOD1 WT ) had any negative effect in either a wild-type or a Gem3 mutant background (Fig. 4). Expression of pathogenic variants including hSOD1 A4V or hSOD1 G85R gave a similar result, hence they were not damaging in either genetic background (Fig. 4). Interestingly, we were surprised to note that less than 50% reduction in enzymatic activity, brought about by heterozygosity for the missense allele Sod1 G51S (+/Sod1 G51S ) 80 , induced a prominent decrease in neuromuscular function during late adulthood in Gem3 mutant flies, suggesting that these organisms are susceptible to oxidative stress when they get old. We confirmed this result through the use of muscle-specific RNAi-mediated loss of Sod1 function in wild-type versus Gem3 BART flies. Hence, we show that an RNAi transgene targeting the C-terminus (Sod1-RNAi [Pembroke], Supplementary Fig. S1) similarly provoked flight defects in adult flies aged to day 35 post-eclosion (Fig. 5). Motor defects became apparent at an even earlier stage (day 25 post-eclosion) when we made use of a stronger RNAi transgene targeting the same region but having a longer hairpin sequence (Sod1-RNAi [Hertford], Fig. 5 and Supplementary Fig. S1). Overall, these findings demonstrate that Gem3 mutant phenotypes are hastened by Sod1 loss-of-function rather than by gain-of-function, hence allowing us to uncover a genetic interaction between Gemin3 and Sod1.

TDP-43 or FUS gain of function enhance muscle atrophy and suppresses neuromuscular junction overgrowth in Gem3 mutant flies.
Above we showed that upregulation of caz or TBPH, in flies with Gem3 loss of function, induced adult lethality. Overexpression of the respective human homologue gave an analogous outcome. We next investigated whether the neuromuscular function of these animals is perturbed prior to their death. Gem3 BART animals devoid of any genetic modifying factor(s) eclose normally and neuromuscular function is relatively unperturbed in adult flies. Surprisingly, when analysing larval crawling, we observed a slight yet significant decline in mobility in Gem3 BART larvae compared to their wild-type counterparts (Fig. 6A). Notably, this difference can be explained by a substantial difference in muscle surface area between Gem3 mutant and control larvae. Thus, the former had a pronounced reduction in muscle size (Fig. 6B). We asked whether these phenotypes are amenable to modification by genetic factors. To this end, we introduced Gaulos RNAi in Gem3 mutants. Gaulos (Glos) was recently identified as the Drosophila orthologue of Gemin4 67,81 , a putative co-factor of Gemin3 82 . Remarkably, muscle-driven Glos reduction caused a further decline in both the locomotor ability (Fig. 6A) and muscle size of Gem3 BART larvae (Fig. 6B), hence confirming that the phenotypes can be genetically enhanced. Importantly, we demonstrate that compared to the baseline provided by Gem3 BART larvae, ectopic expression of either hTDP-43 or hFUS was responsible for an additional degree of sluggishness in larvae as demonstrated by their less frequent movements (Fig. 6A). Interestingly, the difference was reflected in muscle size, hence muscle atrophy was greatly enhanced upon TDP-43 or FUS gain-of-function (Fig. 6B). Upregulation of TBPH causes Gem3 BART flies to die before the third instar larval stage (Table 1), hence precluding assessment of flies. Upregulation of caz in Gem3 BART flies induces larval mobility defects but has no effect on muscle size (data not shown). However, overexpression of the pathogenic variant caz P398L in Gem3 BART flies was found to mirror the neuromuscular phenotypes observed on TDP-43/FUS gain of function in the same genetic background (Fig. 6A,B).
Consequent to muscle atrophy and the subsequent decline in muscle power, all genotypes assessed failed to contract adequately during pupariation. Hence, in a Gem3 BART genetic background, Glos knockdown (serving as a positive control) or overexpression of hTDP-43/hFUS/caz P398L , all induced a puparial axial ratio that was significantly higher than the baseline observed in Gem3 mutant flies devoid of any genetic manipulation (Fig. 6C). Finally, we assessed the morphology of the neuromuscular junction (NMJ). Muscle-directed expression of Gem3 BART causes an appreciative expansion of the NMJ (Fig. 7A), hence, parameters including area (Fig. 7B), number of branches (Fig. 7C) and bouton numbers (Fig. 7D) were all significantly elevated compared to the wild-type control. This phenotype was similar to that previously reported for flies with homozygous Gem3 loss-of-function in all tissues 65 . NMJ overgrowth was also observed on Glos knockdown and upon expression of caz P398L , both of them directed to muscle (Fig. 7). No deviations from the wild-type NMJ parameters were however seen on expression of either hTDP-43 or hFUS in muscle (Fig. 7). Interestingly, in a Gem3 BART background, expression of hTDP-43, hFUS and, to a slightly lower degree, caz P398L (but not Glos RNAi) suppresses the NMJ overgrowth phenotype associated with Gem3 loss-of-function, with key NMJ morphology parameters reduced to the wild-type range (Fig. 7). In sum, these observations strengthen the evidence favouring the possibility that Gemin3 acts together with TBPH/TDP-43 and caz/FUS within a genetic pathway that influences viability and neuromuscular function. www.nature.com/scientificreports www.nature.com/scientificreports/ Self-association of full-length Gemin3 explains loss of function induced by the Gem3 BARt mutant. In conclusion, we wished to gain insights into the mode of action of the Gem3 BART allele, a hypomorphic or weaker version of the Gem3 ΔN mutant. The latter differs from wild-type Gemin3 in that it lacks the N-terminus which hosts the helicase domains 68 . Previously, we have shown that expression of Gem3 ΔN mimics Gem3 knockdown and, together, the genetic alterations cause lethality. This allowed us to conclude that expression of Gem3 ΔN in a wild-type background induces a loss of Gemin3 function 64 . We hypothesised that Gem3 ΔN interacts with its wild-type full-length counterpart and in so doing, it interferes with its function. This model assumes that Gemin3 is capable of self-association for which the evidence is presently lacking. Through a yeast two-hybrid assay, we confirm this model, hence, we show that Drosophila Gemin3 is able to strongly interact with itself (Fig. 8A). Although this property is lost with Gem3 ΔN , remarkably, we demonstrate that Gem3 ΔN is capable of binding to its full-length counterpart (Fig. 8A).
We next questioned whether the Gem3 ΔN -Gem3 interaction is stronger than the one between wild-type Gemin3 monomers, hence favouring the capture of endogenous Gemin3 into non-functional oligomers. To this end, we re-performed the yeast two-hybrid assay in the presence of 3-amino-1,2,4-triazole (3-AT). 3-AT www.nature.com/scientificreports www.nature.com/scientificreports/ competitively inhibits imidazole glycerol-phosphate dehydratase, a histidine (His) biosynthetic enzyme, thus limiting His synthesis 83,84 . We demonstrate that yeast containing both the Gem3 bait and the Gem3 ΔN prey were capable of growing on -Leu-Trp-His selective plates at 20 mM 3-AT (Fig. 8B), thus indicating that the two-hybrid Gem3-Gem3 ΔN interaction is strong enough to overcome the growth inhibitory effect of 3-AT in the medium. In summation, these findings are first supportive of Gemin3 self-binding. Second, they allow us to postulate that the dominant-negative nature of Gem3 ΔN arises from its most-favourable binding to endogenous Gemin3. This is predicted to titre wild-type Gemin3 into non-functional dimers or oligomers.

Discussion
In this study, we sought to determine whether a functional interaction exists between Gemin3, a core SMN complex component, and major ALS-associated proteins. Focusing on motor behaviour, muscle mass, NMJ structure and survival, which are all profoundly affected in motor neuron disease, we show that disruption of either TBPH/ TDP-43 or Caz/FUS enhance muscle defects but are able to suppress NMJ morphology deficits, both induced by Gemin3 loss-of-function. We also found that depletion of Sod1 has an enhancing effect on neuromuscular function in old age. In addition to highlighting shared pathways most likely involving aspects of ribostasis and oxidative stress, our findings reinforce the link between SMA and ALS. Importantly, they extend our knowledge on the function of Gemin3, showing for the first time that it self-interacts, which is a property that makes it prone to loss of function. www.nature.com/scientificreports www.nature.com/scientificreports/ Defective chaperoning of spliceosome assembly and/or missplicing have long been known to have a major role in the pathophysiology of motor neuron degeneration. A plethora of in vivo studies unequivocally show that snRNP assembly defects and the consequential missplicing events can induce the selective motor phenotype that is typical in SMA patients (reviewed in ref. 6 ). In this regard, we have recently shown that, in Drosophila, perturbation of snRNP biogenesis factors pICln or Tgs1 causes motor deficits that mirror those brought about by loss of SMN or select Gemins including Gemin3 61 . This corroborates earlier findings demonstrating that knockdown of pICln or U1 snRNP leads to MND-like phenotypes in zebrafish 85,86 . Notably, by discovering an interaction between Gemin3 and either pICln or Tgs1 61 , we underscored that these factors participate in a common pathway that most likely centres on the synthesis of snRNPs which form the backbone of the spliceosome. Here, we widen our findings by uncovering a genetic association between Gemin3 and two RBPs with important roles in diverse Figure 6. Overexpression of hTDP-43 or hFUS/caz P398L in Gem3 mutant flies causes early mobility defects, reduced muscle size and aberrant puparial structures. (A) Third instar larvae with muscle-specific Gem3 BART expression have a significant reduction in velocity compared to wild-type controls. This deficit was profoundly enhanced on overexpression of hTDP-43 WT , hFUS WT or caz P398L . A similar result was obtained on knockdown of Gaulos (Glos RNAi), which served as a positive control. In the absence of Gem3 BART , only overexpression of hTDP-43 WT or hFUS WT was found to induce a decrease in larval mobility compared to wild-type animals. (B) Compared to the wild-type control, Gem3 BART larvae also show a significant reduction in muscle size that dramatically worsens on Glos knockdown or upon overexpression of hTDP-43 WT , hFUS WT , or caz P398L , all directed to muscle. In a wild-type background, muscle size was also found reduced in larvae with Glos knockdown or those overexpressing FUS/caz P398L . (C) Sluggish larval behaviour leads to the formation of puparia that have a significantly higher axial ratio (defined as length/width) compared to the baseline offered by Gem3 BART larvae. In (A-C) data presented are the mean ± S.E.M. of at least 3 independent experiments, and n ≥ 15 per genotype. Equal number of male and female larvae were used in each assay. Significance as tested by the unpaired t-test is indicated by the exact p-value, shown either in blue (comparison to wild-type larvae) or black (comparison to Gem3 BART larvae). www.nature.com/scientificreports www.nature.com/scientificreports/ . Visual inspection reveals that compared to wild-type, muscle-directed expression of Gem3 BART induces an overgrown NMJ morphology that is restored on overexpression of hTDP-43 WT , hFUS WT and, to a lesser degree, caz P398L . An overgrowth phenotype is also obvious on Glos knockdown (applied singularly or combined with Gem3 BART ) or in flies expressing caz P398L . These observations were confirmed upon quantification of NMJ area (B), number of branches per NMJ (C) and number of boutons within a single NMJ (D). In (B-D) data presented are the mean ± S.E.M. and n ≥ 18 per genotype. Equal number of male and female larvae were assessed. Significance as tested by the unpaired t-test is indicated by the exact p-value, shown either in blue (comparison to wild-type larvae) or black (comparison to Gem3 BART larvae).

Figure 8.
Full-length Gemin3 interacts not only with itself but also with Gem3 ΔN . (A) Measurement of β-galactosidase activity was used to assay the expression of the GAL1-LacZ reporter gene that is produced by the combinations of the indicated proteins. Full-length Gemin3 interacts with itself or with its N-terminal truncated form (Gem3 ΔN ). Gem3 ΔN is not capable of self-binding. An empty vector served as a negative control. Individual bars represent the mean ± S.E.M. β-galactosidase activity of 3 independent experiments. Significance as tested by one-way ANOVA is indicated by the exact p-value. (B) The reporter strain carrying yeast twohybrid plasmids expressing the indicated proteins was spotted in serial dilutions on -Leu-Trp-His plates in the presence of 20 mM 3-AT. Under these conditions, Gem3-Gem3 interaction is unfavourable whereas the Gem3-Gem3 ΔN association remains favourable. Interaction between Gem3 or Gem3 ΔN with Gemin2 served as a negative control. At least 3 independent experiments were performed, and the result of one representative is shown. (C) Model for Gemin3 dimerisation. Left, Gemin3 monomers are hypothesised to bind to each other in the reverse direction. Middle, The Gem3 ΔN mutant is a truncated version of Gemin3 lacking the N-terminus, which hosts the helicase domains. In the presence of Gem3 ΔN , wild-type:mutant dimers are favoured more than wild-type:wild-type dimers. Right, A model predicting self-association in the opposite orientation infers that Gem3 ΔN is not self-binding, hence, mutant:mutant dimers do not form. Abbreviations: N, N-terminus; C, C-terminus. (2019) 9:18666 | https://doi.org/10.1038/s41598-019-53508-4 www.nature.com/scientificreports www.nature.com/scientificreports/ aspects of RNA metabolism, namely TDP-43 and FUS. Our data suggest that Gemin3, TDP-43 and FUS function in overlapping pathways that influence viability, muscle mass, NMJ morphology and motoric ability.
Considering the roles of Gemin3, TDP-43 and FUS at different points in the life of the spliceosome, we speculate that the intersecting pathways are vital for the correct splicing of mRNAs, which we believe is a main contributor to the health and optimal function of the neuromuscular system. In support, although its exact activities in snRNP assembly remain unclear, Gemin3 is indispensable for this process in vivo 60 . Considering TDP-43 and FUS, long before their implication in ALS, these two proteins were reported to influence pre-mRNA splicing or interact with known splicing factors 87,88 . Both RBPs were later shown to bind to predominantly UG-rich sequences in RNA transcripts, regulating the expression and alternative splicing of multiple yet distinct target genes, particularly those with exceptionally long introns [89][90][91][92][93] . Thus, loss of TDP-43 or FUS in cell lines or mouse brain leads to splicing defects that are mostly different for either factor 89,90,92,94,95 . Even splicing of snRNP components is altered based on studies in sporadic ALS patient-derived lower motor neurons that had nuclear TDP-43 depletion 96 or FUS knockdown in a human cell line 97 . Overexpression of ALS-causing TDP-43 or FUS mutants, which cause neuromuscular phenotypes in mice, were also found to induce aberrant RNA splicing 98,99 . Similarly, Drosophila with knockout or overexpression of TBPH exhibit splicing alterations 100 . Interestingly, expression levels of several snRNAs were found significantly increased in brains of flies overexpressing human TDP-43 101 and decreased in fibroblasts derived from ALS patients with FUS mutations or FUS transgenic mice 19 . Notably, in an ALS mouse model, an endogenous C-terminal domain mutation in TDP-43 was recently reported to induce a gain of splicing function. Hence, splicing activity of TDP-43 was modified in such a way that it leads to the excision of otherwise normally conserved exons, thereby, termed 'skiptic exons' 102 . Pathogenic TDP-43 or FUS mutations are also known to affect splicing in a gain-of-toxic-function manner by mislocalising snRNPs, SMN and splicing factors (PSF and NeuN) to the cytosol 18,19,86,103,104 .
It is interesting to note that whereas gain of TDP-43 or FUS function were both shown to enhance Gemin3 motor deficits, we observed that loss-of-function was consequential only for FUS. This can be explained by the more intimate relationship of FUS with the SMN complex. In this respect, in addition to U1 (and U11) snRNP components 19,103 , SMN complex members including SMN and Gemin3 were shown to be integral members of the FUS interactome 19 . It is plausible that the SMN complex might collaborate with FUS in an as yet unknown snRNP-related function, which is disrupted by deviations from normal Caz/FUS levels. Thus, ALS-causative mutations in FUS were found to strengthen the interaction with SMN potentially sequestering SMN and, most probably, its associates, from their normal localisation and function 19 . It is important to note that we do not exclude the possibility that Gemin3 cooperates with TDP-43 and FUS in other steps of RNA metabolism including transcription or RNA transport given that all three factors are known to participate in either process 11,12,28,60,105 .
Gem3 mutant phenotypes were not hastened by SOD1 or C9orf72 gain-of-function. This allows us to infer that the genetic interaction between Gemin3 and TBPH/TDP-43 or caz/FUS is specific. Nonetheless, reduced levels of Sod1 brought about by heterozygosity for a Sod1 mutant or RNAi-mediated knockdown were surprisingly found to induce motor deficits in Gem3 mutant flies during late adulthood. Oxidative stress is exacerbated by age 106 and paucity of Sod1 [107][108][109] . Notably, snRNP assembly function of the SMN complex was found to be inhibited by oxidative stress in a dose-dependent manner 110 . This observation adds to the plethora of evidence showing that oxidative stress perturbs RNA metabolism (reviewed in ref. 111 ). Thus, it is reasonable to speculate that oxidative stress is a modifying factor for Gemin3 function in snRNP synthesis as part of the SMN complex.
Ectopic overexpression of the helicase core deletion mutant Gem3 ΔN in a wild-type background induces phenotypes that overlap those resulting from Gemin3 loss-of-function. In this regard, either overexpression of Gem3 ΔN or RNAi-mediated knockdown of Gemin3, both targeted to muscle tissue, was shown previously to disrupt motor behaviour. Applied simultaneously, these two genetic manipulations were found to cause lethality 64 . Thus, the evidence favours the possibility that Gem3 ΔN interferes with endogenous Gemin3 to induce loss of function, hence acting as a dominant-negative mutant or a Muller's antimorph. Here, we show that Gemin3 is capable of self-interaction and Gem3 ΔN retains the ability to interact with its wild-type counterpart. Importantly, analysis of interaction strength demonstrates that wild-type:mutant dimers are favoured more than wild-type:wild-type dimers (Fig. 8C). Based on these findings, we predict that Gem3 ΔN titres endogenous Gemin3 into non-functional dimers or oligomers. Sequestration of Gemin3 can perturb SMN complex stoichiometry in addition to inhibiting the participation of Gemin3 in SMN complex-related activities including snRNP assembly or recycling.
The formation of wild-type:mutant dimers or even oligomers can potentially hinder the catalytic activity of Gemin3, thus raising the question of whether dimerization of Gemin3 is a prerequisite for its ATPase-dependent RNP chaperoning activities. Self-interaction that is independent of the RNA substrate is rather unusual for members of the DEAD-box RNA helicase family. It has been reported in prokaryotes for Escherichia coli RhIB 112 , Thermus thermophilus Hera 113 , Bacillus subtilis CshA 114 and cyanobacteria CrhR 115 . In this context, our findings add Gemin3 to the growing list of eukaryotic DEAD-box RNA helicases that also have a self-interaction property including transcriptional regulators DDX5/p68 and DDX17/p72 116 . Domain analysis of both T. thermophilus Hera and B. Subtilis CshA revealed that efficient dimerization is dependent on protein regions other than the those hosting the two highly-conserved RecA-like helicase domains which are required for RNA substrate binding and catalytic activity 113,114 . For E. coli RhIB, cyanobacteria CrhR 115 and eukaryotic DDX5 or DDX17, a large part of the conserved core was required for self-association 112,116 , a finding that also applies for Gemin3. To this end, we show that, alone, the C-terminal domain of Gemin3 is incapable of self-binding, hence, the formation of Gem3 ΔN homodimers is an unfavourable reaction (Fig. 8C). However, interaction is observed in the presence of the N-terminus, making the formation of Gem3 ΔN :Gem3 dimers a highly favourable reaction. This indicates that self-interaction requires that the full-length protein is present in at least one of the two monomers, thus raising the possibility that Gemin3 monomers bind to each other in the reverse direction. This model warrants future investigation through further molecular and structural studies. (2019) 9:18666 | https://doi.org/10.1038/s41598-019-53508-4 www.nature.com/scientificreports www.nature.com/scientificreports/ Given the functional interaction of ALS-linked TBPH/TDP-43, Caz/FUS and Sod1 with Gemin3, which itself is intimately associated with the SMA-causative SMN, our work adds to the substantial collection of evidence supporting convergence of the molecular mechanisms of two major MNDs. Although we speculate that defects in RNA metabolism might be central to the pathophysiology of ALS and SMA, further investigation of the mechanistic overlaps is now possible in a genetically-tractable model organism. Importantly, given our findings, we propose Gemin3 as a candidate for modifying motor neuron degeneration.

Materials and Methods
Flies. Flies were cultured on food consisting of sugar, corn meal, yeast, and agar in plastic vials at an incubation temperature of 25 °C under 12 hours day/night cycles. The wild-type strain was w 1118 . For adult-based assays, male flies were used except where indicated. In instances where females were used, flies were virgins. For larval-based assays, equal number of male and female larvae were assessed. Inducible transgenes were expressed via the bipartite GAL4/upstream activation sequence (UAS) system (reviewed in ref. 117 ). Muscle-exclusive expression was achieved through the use of the Mef2-GAL4 driver 118 . The Gem3 BART allele (UAS.Gem3 BART ) was generated previously by transposition of the Gem3 ΔN transgenic allele into a repressive region on chromosome 2 68 . The UAS.Glos-IR DEX (Glos RNAi) transgene was described and characterised previously 67 . TBPH Δ23 and caz 1 are small deletions that partially remove the coding and 5' sequence of TBPH 73 and caz 75 , respectively. They are considered as null alleles of the respective gene. The Sod1 G51S mutant (also known as Sod1 n108 or Sod1 n1 ) carries an EMS-generated missense mutation in the Sod1 gene that disrupts dimer contact 119 . Sod1 G51S homozygotes were reported to be null for superoxide dismutase activity. In Sod1 G51S heterozygotes, superoxide dismutase activity was reported to be close to 40% 80 123 . Combination of the various genetic tools including GAL4 drivers, alleles, and transgenes was performed according to standard genetic crossing schemes.
Mobility assays. Mobility assays in larvae and adult flies were conducted as described previously 67 . In brief, third instar larvae were first placed on a 0.7% agar plate. Subsequently, the number of forward body wall contractions exhibited by the organism in 30 seconds were counted. Each larva was assessed three times before an average was taken. To assess climbing performance in adult flies, two empty polystyrene tubes were vertically joined by tape facing each other. Flies (15)(16)(17)(18)(19)(20) were then transferred into the lower tube and allowed to acclimatize. Flies were then gently tapped down to the bottom of the vial. The time for the first fly within a group to cross an 8 cm threshold was first measured. Consequently, the number of flies per group, that can climb above the 8 cm mark by 10 seconds, was determined. For each group of flies, four trials were performed. A minimum of four groups were assayed per genotype.
Flight assay. Flight performance was assessed as detailed previously 61,67 . This assay made use of the Droso-Drome apparatus, which consists of a 1 L glass bottle coated with an alcohol-based sticky fluid, and divided into 4 sectors, of 5 cm each, spanning a total height of 20 cm. In short, flies first underwent a 'warm-up' by inducing negative geotaxis in an empty tube for 6 times. Organisms were then dropped into the Droso-Drome to induce flight. The number of flies stuck to each sector was next counted, divided by the total number of flies dropped and multiplied by 100 to generate the percentage number of flies per sector. Fight ability correlates with the height or sector in which flies are distributed, hence, fly percentages that are skewed towards the lower sectors of the Droso-Drome are indicative of reduced flight capacity.
Puparial axial ratios. Length and width of puparia were first measured from still images. As reported previously 61,67 , calculation of puparial axial ratios involved dividing the length by the width of the puparia.
Immunohistochemistry. The same immunohistochemistry procedures described previously 65 were followed. Briefly, body wall muscles of wandering third instar larvae were dissected in phosphate buffered saline (PBS), fixed in 4% paraformaldehyde in PBS and washed in PBS + 0.1% Triton X-100 (PBT). Tissues were then stained overnight at room temperature by mouse anti-Discs large antibody (1:1000; Developmental Studies Hybridoma Bank, University of Iowa, USA). On the following day, tissues were washed in PBT and stained overnight at room temperature with anti-mouse Alexa Fluor 488-conjugated secondary goat antibody (1:50) and Alexa Fluor 546-conjugated Phalloidin (1:50). After a final wash in PBT, the samples were mounted in 90% glycerol with anti-fade. Imaging was performed with Optika B-600TiFL microscope (20x or 40x objectives).
Analysis of muscle size and NMJ morphology. ImageJ software (NIH) was used to quantify both muscle and NMJ area. The former comprised of both ventral longitudinal muscles 6 and 7 derived from abdominal segments 2-4 whereas the latter constituted the postsynaptic region on the same muscles stained by the anti-Discs large antibody. Branch number was determined by counting the number of arborisations containing at least two boutons within a single NMJ. To determine, bouton numbers, all boutons were counted within a single NMJ.
Yeast two-hybrid assays. Two-hybrid assays were performed as described previously 67 . Briefly, baits and preys were obtained by PCR amplification of cDNA and ligation into the pAS∆∆ and pACT2st vectors, respectively 124 . Primer sequences and PCR regimes are available upon request. The cDNA clones for Gemin2 (LD47479)