Figure 3 | Scientific Reports

Figure 3

From: Cytogenetic markers reveal a reinforcement of variation in the tension zone between chromosome races in the brachypterous grasshopper Podisma sapporensis Shir. on Hokkaido Island

Figure 3

Meiosis cells with B chromosomes subjected to FISH with 18S rDNA (green) and telomeric DNA (red) probes (ad), and C-banding (a′–d′) show some differences between individuals: X0-5 (a,a′), X0-7 (b,b′), X0-8 (c,c′) and X0-12 (d,d′). In diakinesis, Bs shows differences in the intensity of the hybridization signal (ad; an enlarged image in the insert in the bottom left corner); early diplotene, Bs connected with the X [in the bottom left corner (a′)]; two (b′) or one univalent/s with different patterns of heterochromatin (c′); Bs bivalent in diakinesis with rDNA cluster [in the bottom left corner (d)] and two univalents in metaphase I [bottom panel in the middle (d)] and the heterochromatin pattern (d′). Additionally, some bivalent/s and sex chromosomes indicate the presence of rDNA clusters and C-bands; the hybridization signals and position of C-bands vary in size/position between homologous chromosomes (compare with Table 1) which are marked with an asterisk (*); in the insert on the top of the right corner, the arrows indicate ITSs on the L2 bivalent (c). Scale bar = 10 µm.

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