Figure 5 | Scientific Reports

Figure 5

From: Additive manufacturing of laminar flow cells for single-molecule experiments

Figure 5

DNA dumbbell construction. Step-by-step process of DNA dumbbell construction and exposure to intercalating dye in a flow-free environment using reservoir-based LFC. (1) Individual trapping of DNA-attached streptavidin coated (diameter: 1.76 µm) and free anti-digoxigenin coated beads (diameter: 0.9 µm) within the reservoir and translocating into the main channel; (top-right inset: bright-field image of the trapped beads) (2) applying gentle flow (0.05 mm/s) for a few seconds to elongate DNA and attachment to second bead (3) Incubation of the DNA dumbbell with YOYO-1 in a flow-free reservoir. (4) Visualization of the DNA in the flow-free main channel; bottom-left inset: fluorescent image of DNA dumbbell.

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