Effects of Shmt2 knockout on the growth of livers and brains from E13.5 embryos. (a) Comparison of the weights of tissues or whole embryos between wild-type and Shmt2-knockout mice. WT, wild-type foetal livers (filled blue bars), wild-type foetal brains (open blue bars), and wild-type whole embryos (a hatched blue bar). KO, Shmt2-knockout foetal livers (filled red bars), Shmt2-knockout foetal brains (open red bars), and Shmt2-knockout whole embryos (a hatched red bar). Data represent the means ± S.D. ****P < 0.0001, Student’s t test (WT, n = 26; KO, n = 22). (b) Comparison of the total numbers of cells between wildtype and Shmt2-knockout foetal livers. All cells in each liver from an E13.5 embryo were suspended in 1 mL of PBS containing 5% FBS by a syringe with a 26-gauge needle. WT, wildtype foetal livers (filled blue bars); KO, Shmt2-knockout foetal livers (filled red bars). Data represent the mean ± S.D. ****P < 0.0001, 2-way ANOVA (WT, n = 3; KO, n = 4).