Table 2 Millimolar (mM) concentrations of metabolites present in the cell-free supernatants of mMRS containing 1% lactose, L-fucose, 2′-FL or 3-FL, following 24 hours incubation with B. breve UCC2003 WT, B. kashiwanohense APCKJ1 WT, B. breve UCC2003 fumA1-fumST1T2, or with no inoculum (controls). 2′-FL, 2′-fucosyllactose; 3-FL, 3-fucosyllactose; aGluc, α-glucose; ND, not detected.

From: Metabolism of the predominant human milk oligosaccharide fucosyllactose by an infant gut commensal

Culture ID 1,2-propanediol Ethanol Lactate Acetate Methanol Formate L-Fucose 2′-FL 3′-FL aGluc
No inoculum 1% lactose ND ND 3.5 mM 3.8 mM ND 1.0 mM ND ND ND 1.3 mM
No inoculum 1% fucose ND ND 3.8 mM 3.7 mM ND 1.0 mM 38.4 mM ND ND 1.3 mM
No inoculum 1% 2′-FL ND ND 3.5 mM 3.7 mM ND 1.0 mM ND 20.47 mM ND 1.3 mM
No inoculum 1% 3-FL ND ND 3.5 mM 3.8 mM 1.3 mM 1.0 mM ND ND 19.8 mM 1.5 mM
No inoculum mMRS only ND ND 3.6 mM 4.0 mM ND 1.0 mM ND ND ND 1.3 mM
B. breve UCC2003 WT 1% lactose ND 2.0 mM 6.7 mM 87.0 mM ND 4.0 mM ND ND ND 1.3 mM
B. breve UCC2003 WT 1% fucose 20.7 mM 6.7 mM 3.4 mM 25.4 mM ND 19.7 mM 22.1 mM ND ND ND
B. breve UCC2003 WT 1% 2′-FL ND 1.3 mM 3.4 mM 6.7 mM ND 1.6 mM 0.6 mM 20.4 mM ND 1.2 mM
B. breve UCC2003 WT 1% 3-FL ND trace 3.4 mM 7.5 mM 1.3 mM 1.6 mM ND ND 20.5 mM 2.0 mM
B. kashiwanohense APCKJ1 WT 1% lactose ND 1.7 mM 6.6 mM 91.7 mM ND 3.0 mM ND ND ND 1.5 mM
B. kashiwanohense APCKJ1 WT 1% fucose 1.7 mM 2.0 mM 3.3 mM 9.7 mM ND 5.2 mM 36.1 mM ND ND 1.2 mM
B. kashiwanohense APCKJ1 WT 1% 2′FL 4.6 mM 3.8 mM 3.4 mM 62.8 mM ND 9.0 mM ND 3.3 mM ND 0.9 mM
B. kashiwanohense APCKJ1 WT 1% 3′FL 4.6 mM 2.7 mM 6.0 mM 58.9 mM 1.3 mM 8.0 mM ND ND 3.5 mM 1.3 mM
B. breve UCC2003 fumA1-fumST1T2 1% lactose ND 7.9 mM 6.0 mM 84.0 mM ND 2.8 mM ND ND ND 1.3 mM
B. breve UCC2003 fumA1-fumST1T2 1% fucose 9.8 mM 8.6 mM 3.4 mM 14.5 mM ND 10.9 mM 38.3 mM ND ND 1.2 mM
B. breve UCC2003 fumA1-fumST1T2 1% 2′-FL 5.1 mM 9.0 mM 5.4 mM 61.6 mM ND 12.0 mM 10.8 mM ND ND 1.3 mM
B. breve UCC2003 fumA1-fumST1T2 1% 3-FL 7.8 mM 9.9 mM 4.0 mM 68.2 mM 1.3 mM 15.0 mM 7.6 mM ND ND 1.3 mM