Ribi-adjuvanted immunization yields ZBTB32-independent recall responses. (A) Schematic of primary immunization, adoptive transfer, and secondary immunizations. (B) ELISA measurement of memory B cell responses. Zbtb32+/+ and Zbtb32−/− mice were immunized Ribi-adjuvanted NP-CGG and 8–10 weeks later splenocytes were transferred to naïve IgHa recipients. One day later, recipients were immunized intravenously with soluble NP-CGG. Donor IgG1b NP-specific antibodies were quantified by ELISA. Each data point represents an individual mouse. Error bars depict geometric means ± 95% confidence interval. Differences were statistically insignificant as determined by Mann–Whitney test (p = 0.41).