Figure 1 | Scientific Reports

Figure 1

From: Storage media and not extraction method has the biggest impact on recovery of bacteria from the oral microbiome

Figure 1

Diagram of experimental design. Dental plaque was collected from 20 healthy human participants, pooled, homogenized and stored in either of two media (75% ethanol or Bead solution) at −80 °C. At each time point (0, 1 month, 3 months and 6 months), samples were subjected to DNA extraction via two commonly used DNA extraction kits (QIAamp and PowerSoil) followed by Illumina sequencing of 16S gene. For all tested conditions, two pools of dental plaque samples were collected as biological replicate and one technical replicate was conducted in the second pool.

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