Figure 6 | Scientific Reports

Figure 6

From: Beneficial effects of Lactobacillus reuteri 6475 on bone density in male mice is dependent on lymphocytes

Figure 6

Effect of L. reuteri on naïve splenic CD4+ T Cells cytokine expression. Spleens were isolated from male C57BL/6 mice (12–18 weeks) and naïve CD4+ T cells obtained by magnetic separation. Cells were cultured under non-polarising conditions with (a) live or heat killed L. reuteri 6475 at an MOI of 1, 10 or 100 for 4 days and cytokine expression (IL-10, INFγ, IL17A and TGFβ) analyzed by flow cytometry. Statistical analysis performed by 2-way ANOVA with Bonferroni’s multiple comparison test. *p < 0.05, **p < 0.01 compared to control. n = 10.

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