Evaluation of Chitosan-based Dressings in a Swine Model of Artery-Injury-Related Shock

Uncontrolled haemorrhage shock is the highest treatment priority for military trauma surgeons. Injuries to the torso area remain the greatest treatment challenge, since external dressings and compression cannot be used here. Bleeding control strategies may thus offer more effective haemostatic management in these cases. Chitosan, a linear polysaccharide derived from chitin, has been considered as an ideal material for bleeding arrest. This study evaluated the potential of chitosan-based dressings relative to commercial gauze to minimise femoral artery haemorrhage in a swine model. Stable haemostasis was achieved in animals treated with chitosan fibre (CF) or chitosan sponge (CS), resulting in stabilisation of mean arterial pressure and a substantially higher survival rate (100% vs. 0% for gauze). Pigs receiving treatment with CF or CS dressings achieved haemostasis within 3.25 ± 1.26 or 2.67 ± 0.58 min, respectively, significantly more rapidly than with commercial gauze (>100 min). Moreover, the survival of animals treated with chitosan-based dressings was dramatically prolonged (>180 min) relative to controls (60.92 ± 0.69 min). In summary, chitosan-based dressings may be suitable first-line treatments for uncontrolled haemorrhage on the battlefield, and require further investigation into their use as alternatives to traditional dressings in prehospital emergency care.

Currently, there are more than 10 commercially available chitosan-based dressings that are utilised in the acute emergency phase. Chitosan haemostatic dressings are superior to traditional granular/powder agents due to their effective bleeding arrest, ease of use, and precise application to deep, penetrating wounds, as well as possible antibacterial properties and regeneration-stimulating effects 12 . In this context, we tested two novel chitosan-based dressings, chitosan fibre (CF) and chitosan sponge (CS), for use as first aid measures in a swine model of standard arterial haemorrhage. These dressings are made from poly-D-glucosamine and poly-N-acetyl glucosamine derived from natural organic chitosan. Chitosan is a linear amino polysaccharide produced by deacetylation of naturally occurring chitin and exhibits interesting biological, physiological, and pharmacological properties. Chitosan is rich in glycosaminoglycan, which participates in cell-cell and cell-matrix interactions, cell proliferation and migration, and in cytokine and growth factor signalling, thus locally modulating biologic activities 13 . Major bioactivities include the promotion of wound healing, haemostatic activity, immunity enhancement, hypolipidemic activity, mucoadhesion, and antimicrobial activity. Despite its low water solubility, chitosan has become a common substance in biomaterials research and has been used for several applications in the biomedical field.
The soft, nonwoven CF dressing is made of chitosan fibres that can accelerate haemostasis and wound care during arterial haemorrhage. With its unique flexibility, the CF dressing is suitable for various wound surfaces, regardless of location, size, sinus, or depth. Chitosan fibre manufacturing techniques have been established during the past two decades. Generally, CF dressing is prepared using a wet-spinning process that produces fibres by dissolving the polymer in a solvent (e.g. acetic acid), after which the polymer solution is extruded via dies into a nonsolvent (e.g. aqueous sodium hydroxide). The polymer precipitate emerges in the form of fibres that can be washed, drained, and dried. The CF dressing uses the properties of chitosan fibres to combine an adequate porous structure with excellent degradability and mechanical properties.
The CS dressing, which is a light-yellow sponge, is made of natural organic chitosan particles and can be comfortably attached to the skin to seal a wound. Generally, CS dressing is prepared using a freeze-drying method, resulting in a sponge-like form. It keeps the wound sterile for as long as seven days. Additionally, the porosity of the CS dressing allows rapid absorption of a large volume of exudate from the injury site. Following absorption, the exudate interacts with the CS dressing to form a moist environment covering the wound for up to seven days.
This study aims to compare the efficacy of chitosan-based dressings and commercial gauze in a swine model. We explored the potential of these two chitosan-based dressings to minimise femoral artery haemorrhage and achieve immediate haemostasis in a swine model. Based on our results, chitosan-based dressings require further study as alternatives to traditional dressings in prehospital emergency care.

Results
Background physiological and haematological data. Ten animals were randomly assigned to one of three treatment groups. We collected blood samples for physiological analysis. The pre-experiment baseline physiological and haematological measurements are presented in Table 1. There were no significant differences in baseline measurements among the groups.
Haemostatic achievement. To compare the haemostatic effectiveness of CF, CS, and commercial gauze, the time required to achieve haemostasis was calculated from the initial application of the dressing. Initial haemostasis was considered to have occurred when the bleeding had stopped for at least 3 min after compression. The mean time to haemostasis and other haemostatic outcomes are shown in Table 2. Stable haemostasis was achieved with both the CF and the CS dressings, resulting in stabilisation of MAP and a significant improvement in survival, with 100% of animals surviving throughout the experiment. In contrast, 0% of the gauze-treated animals survived for the entire experiment. The pigs treated with CF and CS dressings achieved haemostasis within 3.25 ± 1.26 and 2.67 ± 0.58 min, respectively, which was significantly less than the time to haemostasis with commercial gauze (>100 min; Fig. 1). The quantity of fluid required for the resuscitation of the CF-and CS-treated animals was 30.55 and 31.58 mL/kg, respectively, which was significantly less than for the gauze-treated pigs (188.32 mL/kg; Table 2). When the stability of the haemostasis provided by the CF and CS dressings was examined under simulated walking, there was no recurrence of bleeding in the animals.
Representative MRI images of the treated pigs showed no significant increase in wound temperature after the application of the haemostatic dressings (data not shown). A complete blockage of blood flow was observed in the femoral artery at the wound site in all surviving animals (Fig. 2). Despite this, blood flow through collateral arteries was not significantly affected.  www.nature.com/scientificreports www.nature.com/scientificreports/ Survival. All of the CF-and CS-treated animals survived for at least 180 min after the procedure, significantly longer than those treated with commercial gauze (60.92 ± 0.69 min; Fig. 3), which died by bleeding to death. The average bleeding times for all dressing types are given in Table 2. Notably, there were no differences in survival rate or time between the two chitosan-based dressing treatments.

Discussion
The present study shows that the CF and CS dressings achieved haemostasis effectively, rapidly controlling moderate-to-severe haemorrhage, which represented a great improvement over commercial gauze. The reduced bleeding time resulted in less blood loss and a lower fluid resuscitation rate. It is reasonable to postulate that the large blood loss with commercial gauze, and the subsequent high fluid resuscitation rate, reduced the clotting capacity of the blood, leading to unfavourable outcomes. Both chitosan-based dressings exhibited similar efficacy   Table 2. Outcomes of treating a groin arterial haemorrhage. Data expressed as mean ± SD and analysed by oneway ANOVA. NS = p > 0.1. ANOVA, analysis of variance; NS, not significant.  www.nature.com/scientificreports www.nature.com/scientificreports/ in this haemorrhage model. Previous studies have also shown that CS dressings effectively achieve haemostasis, even in the presence of anticoagulants in the blood. Therefore, we hypothesise that interactions between the positively charged ions in the chitosan and the negatively charged ions in red blood cell membranes may enhance haemagglutination and encourage platelet adhesion and activation via protein adsorption and orientation, thus affecting cellular responses to proteins 14,15 . It has also been demonstrated that the porosity of this dressing facilitates higher ventilation over the injury site, allowing more oxygen to diffuse inward across the skin, thereby stimulating the healing process.
Chitosan characteristically carries cations such as -NH 3 + , which have a positive effect on haemostasis. If attached to the surface of the chitosan-based dressing, the metal cation-chitosan complex enhances the adhesion of erythrocytes and platelets to the injury site. Chitosan also improves blood coagulation efficiency (either stopping bleeding or controlling haemorrhage). Indeed, it appears to demonstrate better haemostatic efficacy than traditional granular/powder agents, even in the presence of anticoagulants such as heparin. Once the bleeding has stopped, the dressing would then form an antibacterial layer to prevent wound infections and provide an appropriate wound-healing environment. Further, such dressings are compatible with human skin and possess properties that allow ventilation of the wound and absorption of the wound exudate. Previous studies have also demonstrated the safety of chitosan. The biocompatibility and biodegradability of chitosan, combined with its haemostatic and anti-infection activity, make it highly useful and advantageous for use in wound dressings.
The swine model of femoral artery haemorrhage we used in this study represented a severe injury to the groin area with partial destruction of the femoral artery, causing a life-threatening haemorrhage that is impossible to control with commercial gauze, and not manageable using a tourniquet. The strength to this study was that the surgical procedures were performed by a researcher who was blinded to group assignment, which increases confidence in the outcome. Although our approach represents a reliable model for examining the efficacy of haemostatic dressings, there are some limitations of this study that should be addressed in future research. Firstly, considering there are many different types of gauze available for haemostatic management of bleeding, the results in this study can be influenced by the gauze type. Secondly, we are unable to establish a comprehensive comparison between chitosan-based dressings and gauze because of lack of packing time in this study. Although chitosan-based dressings significantly reduced time to haemostasis as compared with control, they require more time for packing according to the procedure. Thirdly, this model does not fully mimic battlefield or accident-induced traumatic injuries. The efficacy of the dressings could be affected by changes in environmental or wound conditions. Lastly, given our small sample size (3 different dressings on 10 animals), this should be followed up with additional studies using a larger sample size.
Currently, the biomedical application of most synthetic polymers is limited by insufficient biocompatibility and poor biodegradability. In contrast, natural polymers such as cellulose, chitin, chitosan, and their derivatives have been proven to be biocompatible and biodegradable. Furthermore, the degradation product of chitosan is nontoxic to humans 16 . Chitosan is also useful as a supporting material for tissue engineering. Previous studies have revealed that both chitosan and chitosan oligomers exhibit antibacterial activity, and that chitosan displays greater antibacterial activity than chitosan oligomers 17,18 . Potential future directions should include conducting chitosan-based dressings testing in more challenging animal models, combining chitosan with other materials to achieve stable haemostasis, and modifying the dressings to facilitate handling to further reduce the packing time.
In summary, a large amount of effort has been invested in the investigation of novel haemostatic products. In this study, a swine model of femoral artery haemorrhage was utilised to evaluate the efficacy of CF and CS dressings. This model mimicked a severe injury to the groin area with partial destruction of the femoral artery, causing a life-threatening haemorrhage that is impossible to control with commercial gauze and not manageable www.nature.com/scientificreports www.nature.com/scientificreports/ with a tourniquet. The results revealed that both CF and CS dressings reduced the time to haemostasis and the fluid resuscitation rate relative to commercial gauze, and the CF-and CS-treated animals survived significantly longer than the control animals. Additionally, the two types of chitosan dressing were equally efficacious in mitigating blood loss and promoting survival. Based on these findings, CF and CS dressings may be suitable first-line treatments for uncontrolled haemorrhage on the battlefield, and require further investigation into their use as alternatives to traditional dressings in prehospital emergency care. Experimental dressing. Surgical cotton gauze was purchased from China Surgical Dressings Company (R.O.C.) and was applied over the haemostatic dressing to fill the wound cavity. The two novel chitosan-based dressings that were used in the experimental groups were purchased from Coreleader Biotech Corporation (CS dressing; R.O.C.) and the Taiwan Textile Research Institute (CF dressing; R.O.C.). The composite fibres were cut to restrict the crimped fibres or yarns to a staple length between approximately 0.25 and 6.35 cm. The staples were carded to create nonwoven attenuated mats or batts, which were then needle-punched and cropped to a size of 10 × 10 cm. The nonwoven multi-layered chitosan fabric had a base weight of approximately 1.55-31 mg/cm 2 . The morphology of the chitosan-based dressings is shown in Fig. 4. In vivo methods. We used ten Yorkshire cross-bred pigs (castrated males only) weighing 34-37 kg in this study. The animals were fasted for 12 h prior to surgery, with unlimited access to water. Before surgery, they were injected with buprenorphine (0.025 mg/kg, intramuscular [i.m.]) for analgesia, and glycopyrrolate (0.01 mg/kg, i.m.) to reduce saliva secretion and block bradycardia. The animals were injected with tiletamine-zolazepam (Telazol, 4-6 mg/kg, i.m.) and anesthetised with 5% isoflurane in oxygen via a face mask. The tidal volume and ventilation rate were adjusted to maintain end-tidal carbon dioxide in the range 38-42 mmHg. Anaesthesia was maintained using 1-2% isoflurane in a mixture of oxygen via a ventilator. An intravenous catheter was inserted into the ear vein for venous access, and Lactated Ringer's solution was maintained at a rate of 5 mL kg −1 h −1 .

Methods
Surgical procedures. The right carotid artery was cannulated for blood collection and the monitoring of blood pressure (systolic, diastolic, and mean) and heart rate throughout the experiment. The femoral artery was identified and partially exposed to approximately 5 cm. The overlying abductor muscle was then removed while taking care to avoid injuring the adjacent femoral vein and nerve. A 2 mL volume of 2% lidocaine was applied to the artery for 5 min to reduce arterial vasospasm and dilate the artery to its normal size. For wound-site temperature monitoring, a microelectrode was sutured to the muscle adjacent to the vessel, but at least 2.5 cm away from the arteriotomy site, to minimise interference with the haemostatic treatment. Baseline mean arterial pressure (MAP) and body temperature were recorded every 10 min during the surgery. A stable MAP of 60 mmHg or www.nature.com/scientificreports www.nature.com/scientificreports/ higher was required before we proceeded with the rest of the experiment. The artery was occluded proximally and distally with vascular clamps and an arteriotomy 6 mm in diameter was made on the anterior surface of the artery using a vascular punch (International Biophysics, Austin, TX). The vascular clamps were then removed to allow free bleeding for 45 s (Fig. 5).
Blood sample processing. A sample of 9.0 ml swine blood was collected from a cannulated femoral artery and transferred to tubes containing 1 mL sodium citrate (3.18%). Haemoglobin (HGB), haematocrit (HCT), and platelets (PLT) were tested. Hemocytometric analysis was measured using automated XT-1800 iv (Sysmex Corp., Japan). For the analysis of blood gas (arterial pH and HCO3), blood samples were immediately analysed with Stat Profile ® pHOx ® (Nova Biomedical, Waltham, MA) analyser.
Wound treatment and resuscitation. The arterial injury, dressing application, and compression were performed by the same investigator (Hsu, S.D.) for all experiments to minimise variability. The surgeon was blinded to the type of test material to be used for each animal prior to dressing application. The products were applied according to the manufacturers' instructions. In brief, a package of one of the types of treatment dressing was opened and applied directly over the arterial wound after the 45 s free bleed. After 30 s of compression, a 500 mL volume of Hextend (6% hetastarch in balanced electrolytes plus glucose) was given intravenously for fluid resuscitation to compensate for pre-treatment blood loss. The colloid fluid was administered intravenously at a rate of 100 mL/min to attain a MAP of 65 mmHg (the average baseline blood pressure of anesthetised pigs). After 5 min of compression, haemostasis was observed for 3 min without removal of the laparotomy gauze. If the initial bleeding had not been controlled, the laparotomy gauze and dressing were removed and fresh dressings were applied. Compression was then repeated for 2 min and haemostasis was observed for the next 3 h.
The animals were monitored for up to 3 h or until death, which was pronounced when the end-tidal PCO 2 and MAP fell below 15 mmHg and 20 mmHg, respectively, and remained at these levels for at least 2 min. The survival time was recorded and the final blood samples (arterial) were collected (for haematological measurements) before the animals were euthanised. The surviving animals were scanned using magnetic resonance imaging (MRI) to obtain images of the arterial blood flow and vascular structures in the legs. In addition, the stability of the haemostasis provided by the haemostatic dressings was tested by flexing and stretching the wounded legs of the surviving animals five times, to mimic walking. At the end of the experiments, the dressings were slowly removed from the wounds to examine the status of the haemostatic clots and patency of the vessels. The animals were then euthanised and tissue samples containing the injured artery, adjacent femoral vein, femoral nerve, and muscle tissues were collected for histologic examination. Data analysis. Data were expressed as mean ± standard error of the mean and analysed by one-way Analysis of Variance (ANOVA) test; p < 0.05 was considered statistically significant 19 .