Reduced macrophages, EMH clusters and higher lipid accumulation in the livers of NOD mice. Livers from neonatal and 30 day C57BL/6J and NOD/ShiLtJ mice were labelled using IHC for the macrophage marker, CD68 (a), with Periodic acid–Schiff (b) and with Oil red O for detection of lipids (c). Inset shows magnified areas of lipid accumulation. (d) Quantification of CD68+ area, EMH clusters/mm2 and percentage of Oil red O+ area in B6 (open triangle) and NOD mice (red filled triangles). Data (n = 5–12) were analyzed using unpaired t-test with Welch’s correction (GraphPad 8) and are expressed as mean ± SD.