Prediabetic rats have higher lipid accumulation. (a) Neonatal and 30 day livers from BBc and BBdp rats were stained with Oil red O for detection of lipids (n = 4–9). Inset shows magnified area of lipid accumulation. (b) Percentage of Oil red O+ area in the livers. Serum levels (n = 10) of triglycerides (c) and glucose (d) in neonates and 30 day BBc (open circle) and BBdp rats (red filled circles). Data were analyzed using unpaired t-test with Welch’s correction (GraphPad 8) and are expressed as mean ± SD.