Metabolism related gene and protein expression in neonate and 30 day rat livers. Expression of glucose and lipid metabolism related genes was investigated in liver samples from (a) neonates and (b) 30 day BBc (black open circle) and BBdp rats (red filled circles) (n = 10–12). (c) Summary of genes analyzed using RT-qPCR. PPARγ, AMPKα, pAMPKα protein expression in neonate (d) and 30 day livers (f) (n = 5–6). Quantification was by densitometric analysis of chemiluminescence signal (e,g); proteins of interest were normalized to expression of β-actin on the same blot and individual animals were normalized to the mean value of the control animals (full length blots in Suppl Figs). Data were analyzed using unpaired t-test with Welch’s correction (GraphPad 8) and are expressed as mean ± SD.