Innate immune gene expression in the liver of neonates and 30 day prediabetic rats. (a) Heatmap of Innate and Adaptive Immune Response PCR array gene expression from BBc and BBdp rat neonatal liver. (b) Summary of selected genes from the array showing significant differences (≥1.4 fold-change, p < 0.05) in BBdp rats. Validation of target genes was performed in liver samples (n = 10–12) from (c) neonates and (d) 30 day rats; BBc (open circle) and BBdp rats (red filled circles). (e) Summary of validated genes by RT-qPCR. Data were analyzed using unpaired t-test with Welch’s correction (GraphPad 8) and are expressed as mean ± SD. P values: * ≤ 0.05, ** < 0.01, *** < 0.001, **** < 0.0001.