Macrophages and EMH are decreased in neonatal livers from BBdp rats. (a) Neonatal and 30 day livers from BBc and BBdp rats were labelled with anti-CD68 antibodies. (b) Quantification of the CD68+ area in the liver. (c) Immunofluorescence staining of the liver with the macrophage markers CD68 (red) and CD163 (green). (d) Periodic acid–Schiff staining of neonatal and 30 day livers from BBc and BBdp rats (n = 6–12), yellow arrows indicate extramedullary hematopoiesis (EMH) clusters. (e) Quantification of the number of EMH clusters/mm2. (f) EMH clusters and CD68+ cells. (g) Percentage of CD68+ cells per EMH cluster. (h) Comparison of number of EMH clusters in neonates, 30 day and 60 day BBc and BBdp rats. (For simplicity, data for each age are presented on the same axis). Data were analyzed using unpaired t-test with Welch’s correction (GraphPad 8) and are expressed as mean ± SD.