Milk EVs restore intestinal permeability in vivo. (A) Quantification of dextran-FITC in the plasma of healthy and colitic mice different times after feeding with one of the three feeding solutions. Fluorescence is expressed as fold change versus control. (B) Dextran-FITC in plasma at day 7. Statistical comparison. For all conditions, statistical significance was determined by one-way Anova with Bonferroni’s post-hoc correction for multiple comparison with p < 0:05 considered significant (n = 10/group). Significance display. *p < 0.05; **p < 0.01; ***p < 0.001. Abbreviations. CS, control solution; DSS, dextran sodium sulfate; EVs, extracellular vesicles; FITC, fluorescein isothiocyanate; P100K, pellet 100,000 g; P35K, pellet 35,000 g.