Cobalt(II)-coordination polymers containing glutarates and bipyridyl ligands and their antifungal potential

Three new CoII-coordination polymers (Co-CPs) containing glutarates and bipyridyl ligands, formulated as [Co2(Glu)2(µ-bpa)2]·(H2O)4 (1), [Co4(Glu)4(µ-bpp)2] (2), and [Co2(Glu)2(µ-bpe)2]·(H2O)0.5 (3), were prepared, and their structures were determined by X-ray crystallography. Glutarates bridge CoII ions to form 2D sheets, and the sheets are connected either by bpa or by bpp ligands to form 3D networks 1 and 2, respectively. Both frameworks 1 and 2 are two-fold interpenetrated, and there is no significant void volume in either network. Four glutarates bridge two CoII ions to form chains, and these chains are connected by bpe ligands to form the 2D sheet 3. The antifungal properties of these new Co-CPs were tested against two model fungal pathogens, Candida albicans and Aspergillus niger. Under the maximum concentration of Co-CPs, 2.0 mg mL−1, the inhibition rates of Co-CPs against A. niger were much lower (44–62%) than those (90–99.98%) observed in C. albicans. The results indicate that 1–3 can inactivate C. albicans cells more efficiently than A. niger spores in the same treatment time, and the greater inactivation of C. albicans can be explained by dramatic changes in the morphology of C. albicans cells. We also found that Co-CPs could generate the reactive species NO and H2O2, and these species might play a role in inactivating fungal cells. Additionally, degradation tests confirmed that the leaching of CoII ions from Co-CPs was not significant. The small amount of leached CoII ions and the robust Co-CPs themselves as well as the reactive species generated by Co-CPs can actively participate in fungal inactivation.

hydrazine) 22 . These linkers increased the pore dimensions for the gas sorption behaviour of the frameworks. Solvent-and pressure-induced phase changes in the same 3D Cu-MOFs via glutarate conformational isomerism have also been reported 23 . Recently, these water-stable robust Cu-MOFs containing glutarates and bipyridyl linkers (bpy, bpa, bpe, and bpp) were tested for antibacterial activities against five bacteria 27 . They exhibited excellent antibacterial activities against five kinds of bacteria tested, including both Gram-positive bacteria (Staphylococcus aureus and MRSA) and Gram-negative bacteria (Escherichia coli, Klebsiella pneumonia, and Pseudomonas aeruginosa), with very low MBCs (minimal bactericidal concentration).
As an extension of our previous work, we present here two new 3D Co II -coordination polymers (Co-CPs) containing glutarates and bipyridyl ligands, formulated as [Co 2 (Glu) 2 (µ-bpa) 2 ]·(H 2 O) 4 (1) and [Co 4 (Glu) 4 (µ-bpp) 2 ] (2) (Glu = glutarate) for a systematic study of MOFs and CPs containing glutarates and bipyrdyl ligands. We also present a new 2D Co-CP containing bipyridyl ligands with double bonds, formulated as [Co 2 (Glu) 2 (µ-bpe ) 2 ]·(H 2 O) 0.5 (3). They were prepared by a solvothermal reaction in a DMF solution of the mixture of Co(NO 3 ) 2 , glutaric acid and bpa, bpp, or bpe (Scheme 1). The antifungal properties of these new Co-CPs were tested against two model fungal pathogens, Candida albicans and Aspergillus niger. C. albicans is an opportunistic fungal pathogen that can infect human tissues, causing candidiasis 28 . C. albicans occurs mostly in yeast form but becomes filamentous during infection 29 . A. niger is a filamentous Ascomycete fungus implicated in human and plant infection as well as food contamination and fermentation 29 . This fungus is often found in bakery products, intermediate-moisture food products, cheeses, preserved fruits and grains. Although it has been reported that the water-stable Cu-BTC MOF (BTC = 1,3,5-benzenetricarboxylate) can inhibit the growth rate of C. albicans 30 , and other Cu-based HKUST-1 MOF can inactivate Saccharomyces cerevisiae and its antifungal property is due to releasing Cu II ions from its own structure 31 , Co-based MOFs and CPs have rarely been tested for their antifungal properties. A Co-based MOF (Co-TDM, TDM 8− = [(3,5-dicarboxyphenyl)-oxamethyl]methane) was shown to be highly effective at inactivating E. coli 32 . However, there are few reports on the antifungal activity of Co-based MOFs and CPs.

Results and Discussion
Structure description. Co-CPs 1 and 2 crystallize in the monoclinic P2 1 /n space group, and single-crystal X-ray analysis (Table 1) revealed that the glutarates bridge Co II ions to form two-dimensional (2D) sheets, and the sheets are also connected either by bpa ligands or by bpp ligands to form 3D networks 1 (Fig. 1) and 2 (Fig. 2), respectively. In 1, two glutarates bridge two Co II ions in bridging mode and chelating/bridging mode to form one-dimensional (1D) chains, and these chains are connected by other glutarates in chelating and monodentate coordination modes to form a 2D sheet. The bpa ligands connect these 2D sheets to form a 3D network. The 3D networks are two-fold interpenetrated (Fig. S1a), and there is no significant void volume for solvents (4.9%, 161.5 Å 3 /3297.9 Å 3 on PLATON analysis 33 ). The coordination geometry of one Co II ion is distorted octahedral constructed by one bridging carboxylate O atom (η 1 :η 1 :μ 2 ), two chelating O atoms (η 1 :η 1 ), one monodentate carboxylate O atom (η 1 :η 0 ), and two pyridyl N atoms. That of the other Co II ions is also distorted octahedral constructed by one bridging O atom (η 1 :η 1 :μ 2 ), two chelating O atoms (η 1 :η 1 ), one chelating/bridging O atom (η 2 :η 1 :μ 2 ), and two pyridyl N atoms. The Co-O bond distances range from 1.994(4) to 2.285(4) Å, and the Co-N distances range from 2.150(3) to 2.184(3) Å ( Table 2). The Co···Co distance in a Co 2 unit is 3.804 Å.
For 2, two Co II ions are bridged by three glutarates in two bridging and one chelating/bridging modes, and one of the Co II ions is chelated by the other glutarate to form 2D sheets. These 2D sheets are connected by bpp ligands to form a 3D network. The 3D networks are also two-fold interpenetrated (Fig. S1b), and there is no void volume for solvents. The coordination geometry of one Co II ion is distorted square pyramidal was constructed by two bridging O atoms, two chelating O atoms, and one pyridyl N atom and that of the other Co II ion is distorted octahedral constructed by two bridging O atoms, two chelating O atoms, one chelating/bridging O atom, and one pyridyl N atom. The Co-O bond distances range from 1.952(3) to 2.239(3) Å, and the Co-N distances range from 2.048(3) to 2.154(3) Å ( Table 2). The Co···Co distances in Co 2 units are 3.49 and 3.49 Å.
The 3D networks of both 1 and 2 indicate a 6-connected uninodal net (pcu alpha-Po primitive cubic) with a Schläfli symbol of 4 12 ·6 3 , assuming that the Co 2 unit acts as a node by ToposPro analysis 34 .
The bulk purities of 1-3 were confirmed by powder X-ray diffraction (PXRD) (Figs S2-S4), elemental analysis (see experimental section), and thermogravimetric analysis (TGA) of the as-prepared samples (Figs S5-S7). The scanning electron microscopy (SEM) images of three Co-CPs are shown in Fig. S8. 1 and 3 have rod type crystals, 2 has aggregated crystals. The crystals of all three compounds were used as prepared without grinding. The structural stabilities of 1-3 in water or 1x PBS (Phosphate Buffered Saline) solution have been tested by PXRD, and the PXRD patterns of 1 and 2 indicate that their structures are stable after 4 days in water or PBS solution (Fig. S9). For 3 after 4 days in water or PBS, the intensities of diffraction planes of (011), (101), (112), and (1-1-1) at 2θ = 8.63, 9.54, 13.677, and 14.484°, respectively, were reduced compared with those of the as-prepared sample. Contrarily, the diffraction planes of (110) and (1-10) at 2θ = 11.457 and 11.962° were maintained. These differences of diffraction patterns between the as-prepared sample and samples after 4 days in water or PBS imply that partial degradation of the framework. This also consists with the small amount of Co II ions released from Co-CPs in PBS which will be discussed in the later part.
Antifungal activities. The antifungal properties of the three Co-CPs against C. albicans and A. niger were assessed by using the plate counting method. C. albicans cells were incubated in different concentrations of each compound (2, 1, 0.5, 0.25, and 0.125 mg mL −1 ) for 4 days. When yeasts were incubated with compounds for 1 day, no significant antifungal effects were observed (Fig. S10a). However, after 4 days, the difference in CFU numbers between the control and treatments became more dramatic (Figs 4 and S11). Representative images of C. albicans colonies on PDA (Potato Dextrose Agar) plates are shown in Fig. 5. 1 inactivated C. albicans by approximately 91% at 2 mg mL −1 , 2 by 99.98% at 0.25 mg mL −1 , and 3 by 99.93% at 0.25 mg mL −1 (Figs 4 and S11). 2 and 3 showed relatively higher antifungal activity against C. albicans than 1 (Figs 4 and 5). The result indicates that the viability of yeast cells is reduced by increasing the concentration of Co-CPs, although a relatively longer incubation time is required to obtain a similar level of antifungal effect to that of the previously reported Cu-BTC, in which C. albicans was effectively inactivated with a 500 ppm (0.5 mg mL −1 ) concentration and a short incubation time (60 min) 30 .
A. niger spores were also incubated in different concentrations of each compound (2, 1, 0.5, 0.25, and 0.125 mg mL −1 ) for 4 days, and spore germination was assessed as an indicator of spore viability. As observed  www.nature.com/scientificreports www.nature.com/scientificreports/ in C. albicans, treatment of A. niger spores with compounds for 1 day did not produce any significant antifungal effects (Fig. S10b). In contrast to C. albicans cells, A. niger spores are pigmented, and this pigmentation may affect the responses to Co-CPs. Inactivation of A. niger spores by Co-CPs measured as number and percentage of spore germination was not dramatic compared to that observed in C. albicans (Figs 6 and S12). Representative images of A. niger colonies on PDA plates are shown in Fig. 7. The germination percentage of A. niger spores was more slowly reduced over increased concentrations of compounds compared to the CFU percentage of C. albicans cells (Figs 4 and 6 and S11, S12). At the maximum concentration of compounds, 2.0 mg mL −1 , the inhibitory effect of 1,    www.nature.com/scientificreports www.nature.com/scientificreports/ 2, and 3 against A. niger were approximately 44%, 46%, and 62%, respectively, after incubation for 4 days (Fig. 6). These inhibition rates were much lower than those (90-99.98%) observed in C. albicans (Figs 4 and 6).
Our results indicate that Co-CPs can inactivate C. albicans cells more efficiently than A. niger spores in the same treatment time. The antifungal activity of Co-CPs is likely to be variable depending on fungal species. The differential sensitivity of fungal species to MOFs was also observed in a previous study in which Cu-BTC inhibited spores of F. oxysporum and A. oryzae up to 30% but was not affected by A. niger spores even after 7 days of incubation 30 . Our results can be compared with those of silver(I) CPs and MOFs showing a notable antimicrobial efficiency against a panel of bacteria (E. coli, P. Aeruginosa and S. aureus) and yeast (C. albicans) with the minimum inhibitory concentrations (MIC) of 30-60 μg mL −1 (normalized MIC of 11-68 nmol mL −1 ), but their antimicrobial action is due to release Ag I ions from Ag-MOFs or Ag-CPs [35][36][37][38] . The antifungal activities of Co-CPs can also be compared with those of other nanomaterials: Ag nanoparticles on C. albicans biofilms 39 , ZnO nanoparticles against E. coli 40 , and reduced graphene oxide nanosheets against A. niger, A. oryzae, and F. oxysporum (Fusarium oxysporum) with half-maximal inhibitory concentrations (IC 50 ) of 50, 100, and 100 μg mL −1 , respectively 41 .
Our SEM analysis demonstrated that the morphology of C. albicans cells and A. niger spores was differentially changed after treatment with Co-CPs (Fig. 8). Loosened spore surfaces (probably destruction of the fungal cell wall) without severe changes in cell shape were observed in many A. niger spores, while a large proportion of C. albicans cells were severely crushed (Fig. 8). In A. niger treated with Co-CPs, the spore surface was wrinkled, and the surface layers peeled off (Fig. 8b). This result indicates damage to the spore cell wall, most likely making the spores more vulnerable to mechanical and other environmental stresses. However, we could not see   www.nature.com/scientificreports www.nature.com/scientificreports/  www.nature.com/scientificreports www.nature.com/scientificreports/ wrinkles or peel-off of the surface in C. albicans cells treated with Co-CPs. This may be because of the difference in the composition and structure of the cell wall between C. albicans and A. niger, although both are fungi. More dramatic changes in morphology were observed in C. albicans than in A. niger, which might explain the greater inactivation by Co-CPs in C. albicans. The morphological changes observed after treatment with Co-CPs were not significantly different among the 3 types of Co-CPs (Fig. 8), where 1 and 2 are two-fold-interpenetrated 3D frameworks and 3 is a 2D sheet.
To find the mechanism for antifungal effects, ROS (reactive oxygen species) and RNS (reactive nitrogen species) levels in PBS solution were analysed after treatment with Co-CPs. These Co-CPs can generate ROS/RNS that can interact with biomolecules in fungal spores, producing an inhibitory effect on fungal growth 42 . Due to the www.nature.com/scientificreports www.nature.com/scientificreports/ limitations of the available methods, we could not measure many reactive species. The concentrations of nitric oxide (NO) and hydrogen peroxide (H 2 O 2 ) were measured. The levels of both reactive species increased more dramatically with compound concentration in 1 and 2 than in 3 at day 0 ( Fig. 9). After 4 days, NO concentration was generally elevated in all treatments without a significant change in trend among Co-CPs (Fig. 9). However, a similar level of H 2 O 2 (approximately 12 µM) was observed in all treatment concentrations of 2, while the H 2 O 2 level was decreased in the treatment with higher concentrations of 1 and 3 (Fig. 9). Our data demonstrate that Co-CPs can generate NO and H 2 O 2 , and these species may play a role in inactivating fungal cells. Recently, it is reported that ROS can be generated by photocatalysis of MOFs 43,44 . Photoinduced metal ion activates O 2 , producing ROS such as superoxide or singlet oxygen via electron transfer. Since our experiments were performed in the presence of light, light-induced electron transfer from Co metal to O 2 might produce ROS such as superoxide or singlet oxygen. H 2 O 2 and NO can be further generated through reaction with dissolved oxygen and nitrogen in PBS background solution. However, the relatively lower levels of NO and H 2 O 2 observed in the treatment with 3 are not sufficient to explain the high antifungal activity of 3. Moreover, the H 2 O 2 level was maintained at approximately 12 µM in all treatment concentrations of 2 and reduced with the increased concentration of 1 and 3. These results are not well matched with the antifungal effects. In addition to reactive species, other factors, such as mechanical damage to fungal cells by contact with the surface of Co-CPs or toxicity of glutarate (ligand chemical in Co-CPs) and Co II ion, should also be considered.
Additionally, degradation tests were carried out in the 1x PBS solution used for antifungal testing at room temperature to confirm that the leaching of Co II ions from Co-CPs was not significant. The quantities of Co II ions released from Co-CPs were monitored by ICP-MS (ICP-MS measurements were performed in Seoul Center, Korea Basic Science Institute) after 1 day, 2 days, 3 days and 4 days (Fig. S13). The concentration of released Co II ions from 1 mg of a compound in 1 mL of 1x PBS after 4 days was approximately 0.013 mg mL −1 for all three Co-CPs. The amount of Co II ions released from 3 was calculated to be 0.0033 mg mL −1 , which is negligible compared to 0.25 mg mL −1 of the 99.93% inactivating property. Co-CPs cannot easily release Co II ions and are stable in 1x PBS. From these results, we infer that reactive species from Co-CPs, the small amount of leached Co II ions, and Co-CPs themselves actively participate in fungal inactivation.
To find the toxicity of glutarate and Co II ion, we incubated C. albicans cells and A. niger spores in PBS solutions containing glutarate (2 mg/ml, equivalent to Co-CPs concentration showing the highest antifungal activity) and Co salt (0.02 mg/ml, equivalent to the released Co II concentration after 4 days) for 4 days and measured the fungal viability. Glutarate did not affect significantly the viability of both fungal cells (Fig. S14). However, treatment with Co salt reduced the viability of C. albicans cells up to around 50% while no significant effect on A. niger spores was observed (Fig. S14). Although reduction in C. albicans cell viability by Co-CPs (over 90%) was much higher than that by Co salt (around 50%), Co II ion released from Co-CPs could partially contribute to fungal inactivation, probably through the redox activity of Co II ion on biomolecules leading to the fungal cell damage. www.nature.com/scientificreports www.nature.com/scientificreports/ In summary, three new Co-CPs containing glutarates and bipyridyl ligands, formulated as [Co 2 (Glu) 2 (µ-bpa ) 2 ]·(H 2 O) 4 (1), [Co 4 (Glu) 4 (µ-bpp) 2 ] (2), and [Co 2 (Glu) 2 (µ-bpe) 2 ]·(H 2 O) 0.5 (3), were prepared and characterized by X-ray structure determination. Glutarates bridge Co II ions to form 2D sheets, and the sheets were also bridged either by bpa or by bpp ligands to form 3D networks 1 and 2, respectively. Both 1 and 2 are two-fold interpenetrated, and there is no significant void volume in both networks. Four glutarates bridge two Co II ions to form chains, and these chains are connected by bpe ligands to form the 2D sheet 3. The antifungal properties of these new Co-CPs were tested against two model fungal pathogens, C. albicans and A. niger. Under the maximum concentration of Co-CPs, 2.0 mg mL −1 , the inhibition rates of Co-CPs against A. niger were much lower (44-62%) than those (90-99.98%) observed in C. albicans. The results indicate that Co-CPs can inactivate C. albicans cells more efficiently than A. niger spores in the same treatment time, and the greater inactivation of C. albicans by Co-CPs may result from dramatic changes in the morphology of C. albicans. Co-CPs can generate NO and H 2 O 2 , and these species may play a role in inactivating fungal cells. Additionally, degradation tests showed that the leaching of Co II ions from Co-CPs was not significant. These results imply that the small amount of leached Co II ions and Co-CPs themselves as well as reactive species generated by Co-CPs can participate in fungal inactivation. Further investigation into the antifungal mechanisms of Co-CPs and other MOFs will be conducted to obtain a clear conclusion. X-ray crystallography. The X-ray diffraction data for all three Co-CPs were collected on a Bruker APX-II diffractometer equipped with a monochromator in the Mo Kα (λ = 0.71073 Å) incident beam. Each crystal was mounted on a glass fibre. The CCD data were integrated and scaled using the Bruker-SAINT software package, and the structure was solved and refined using SHEXL-2014 45 . All hydrogen atoms were placed in the calculated positions. The crystallographic data for the three compounds are listed in Table 1. The selected bond distances and angles of compounds are listed in Table 2. Structural information was deposited at the Cambridge Crystallographic Data Centre. The CCDC reference numbers are 1561941 for 1, 1561902 for 2, and 1561903 for 3.

Instrumentation.
Thermogravimetric analysis was carried out on a TGA Q5000 (TA Instruments) under a nitrogen atmosphere. The elemental compositions of Co-CP samples were analysed at the Organic Chemistry Research Center, Sogang University (Seoul, Korea) by using EA1112 (CE Instruments, Italy). Powder X-ray diffraction patterns were recorded on a Bruker D8 Focus diffractometer (40 kV, 30 mA, step size = 0.02°). IR spectra were measured on a BIO RAD FTS 135 spectrometer as KBr pellets.
Antifungal assays. We treated C. albicans (KCTC7270) cells and A. niger (130708) spores with 3 types of Co-CPs to assess the antifungal activity of Co-CPs. Both C. albicans and A. niger were maintained and propagated in PDA medium. To obtain C. albicans cells for the assay, 1-2 colonies from PDA plates were suspended in 1 mL of PDB (potato dextrose broth). Then, 10 µL of suspension was inoculated into 15 mL of PDB placed in a 50 ml conical tube. The tube was incubated at 30 °C with shaking for 16-20 h and then centrifuged at 3,134 × g for 5 min. After the liquid part was discarded, the C. albicans cell pellet was washed with 1x PBS. The washed pellet was resuspended in new PBS, and the cell concentration was adjusted to 10 8 per mL.
Spores of A. niger were harvested from 2-to 3-week-old PDA culture plates. Approximately 20 mL of sterile deionized (DI) water was added to each PDA culture plate, and fungal materials were scraped using a plastic scraper. The resulting suspension was filtered through 3 layers of sterile Miracloth (Millipore, Burlington, MA, USA) to remove fungal mycelia. The spore suspension was centrifuged at 3,134 × g for 5 min, and the liquid was www.nature.com/scientificreports www.nature.com/scientificreports/ discarded. The spore pellet was washed with 1x PBS and then resuspended in fresh 1x PBS, and the spore concentration was adjusted to 10 8 per ml.
For treatment with Co-CPs, a suspension of C. albicans cells or A. niger spores was placed in a 24-well plate (1 mL per well). Then, the Co-CP (suspended in PBS) was added to each well to the indicated concentrations (2, 1, 0.5, 0.25, 0.125 mg mL −1 ). The well plate was shaken at room temperature for 4 days. The treated suspension was serially diluted, and then 100 µL of diluted suspension was spread on PDA plates. The plates were incubated at 30 °C for 2 days, and the number of colonies was counted. Three replicates of treatment were performed at each compound concentration, and colonies were counted from 9 replicate plates. Analysis of morphology by scanning electron microscopy (SEM). Morphological changes in fungal cells and spores were analysed using SEM following Co-CP treatment. After treatment with Co-CPs (2 mg mL −1 ) for 4 days, control (no Co-CPs) and treated C. albicans cells and A. niger spores were washed twice with PBS and fixed in Karnovsky's fixative [2% (v/v) paraformaldehyde and 2% (v/v) glutaraldehyde in 1x PBS] overnight at 4 °C. After primary fixation, the fungal cells and spores were washed three times with PBS. The washed cells and spores were mixed with approximately 1 ml of 1% (v/v) osmium tetroxide (Ted Pella Inc., Redding, CA, USA) and incubated for 2 h at room temperature in the dark. The cells and spores were then washed twice with PBS and dehydrated via serial incubations in 30, 50, 70, 80, 90, and 100% (twice) ethanol. In each dehydration step, the cells and spores were mixed with 1 ml of ethanol and incubated for 5 min at 4 °C. After dehydration, 500 µL of hexamethyldisilazane (HMDS) was applied to the samples, and the samples were incubated for 15 min for drying. This drying step was repeated once. Then, the dried cells and spores were mounted on carbon tape, coated with platinum, and examined under a scanning electron microscope (SEM) (JEOL, Tokyo, Japan).

Measurement of NO (nitric oxide) and H 2 o 2 concentration. To analyse NO and H 2 O 2 concentrations
in 1x PBS solution treated with Co-CPs, 1 mL of PBS solution was placed in each well of a plate, and the Co-CPs (suspended in PBS) were added at the indicated concentrations (2, 1, 0.5, 0.25, 0.125 mg mL −1 ). The well plate was shaken at room temperature for 4 days. The concentrations of NO and H 2 O 2 were measured immediately after Co-CPs were added (0 day) and after 4 days. The NO and H 2 O 2 levels were measured using a QuantiChromTM Nitric Oxide Assay Kit (BioAssay Systems, Hayward, CA, USA) and an Amplex ™ Red Hydrogen Peroxide/ Peroxidase Assay Kit (Molecular Probes, Eugene, OR, USA), respectively, following the manufacturer's protocols. co-cp degradation and release of co ii ion. To assess whether Co-CPs will be able to release their constituent Co II ions, 1 mL of 1x PBS solution was added to 1 mg of each compound and stirred for 1 day-4 days at r.t. After different times, each sample was centrifuged, and the supernatant was removed from the reaction tube. The amount of Co II released in the sample was quantified by ICP-MS (ICP-MS measurements were performed in Seoul Center, Korea Basic Science Institute). The degree of degradation is represented as the concentration of Co II ions released into the medium.