Table 1 Composition of perfusate for each circuit.

From: Cold Preflush of Porcine Kidney Grafts Prior to Normothermic Machine Perfusion Aggravates Ischemia Reperfusion Injury

Perfusate
250 ml Ringer’s solution with added:
-Mannitol 5 g/L
-Sodium bicarbonate 2,1 g/L
-Creatinine 0,116 g/L
-Glucose 1,98 g/L
250 ml leucocyte-depleted autologous whole blood
Osmolarity: 290 mosm/L, Oncotic pressure: estim. 8.5 mmHg
Urine replacement
Ringer’s solution
Nutritive solution, added at 11,2 ml/h
Nutriflex peri 44 ml
Cernevit 0,4 ml
Insulin 20 IU
  1. For DNMP kidneys a total of 700 ml of perfusate was prepared and the first 200 ml of venous perfusate were discarded.