Table 2 Organisms used in this study.

From: The involvement of McpB chemoreceptor from Pseudomonas aeruginosa PAO1 in virulence

Bacteria/Nematodes/Arthropods/Annelids Genotype, relevant characteristics and usesa Reference or source
E. coli MC4100 F- araDI39 Δ(argF-lac) U169 rpsL150 relAl flb5301 deoCI ptsF24 rbsR. Used to evaluate the presence of antibiotic compounds in supernatants of PAO1 strains. 56
E. coli OP50 Uracil auxotroph. Used to feed C. elegans. 57
E. coli β2163 KmREmR; F RP4-2-Tc::Mu ΔdapA::(erm-pir). 58
E. coli DH5α supE44 lacU169 (Ø80lacZΔ M15) hsdR17 (rk-mk-) recA1 endA1 gyrA96 thi-1 relA1. 59
B. cepacia CC-Al74 Wild type. Risk Group 2 bacteria and proposed as plant growth promoting rhizobacteria (PGPR). Used as a pathogenic control in virulence assays. 60
P. putida KT2440 Wild type; Risk Group 1 bacteria and PGPR. Used as negative control in virulence assays. 61
V. fischeri ATCC 49387 Wild type; Used as bioluminescent strain in MicroTox® assays. 62
P. aeruginosa PAO1 Wild type pathogenic strain. 9
P. aeruginosa ΔmcpB In-frame deletion mutant in mcpB (1116 bp Δ). This study
C. elegans Bristol strain N2 Nematode used in virulence assays. Provided by the Laboratory of Nematology, National Museum of Nature Sciences-CSIC (Madrid, Spain) Navas et al., 2007
A. bipunctata Arthropod used in virulence assays. Supplied by ControlBio Co. (Almería, Spain). Ref. CBi K04884
C. carnea Arthropod used in virulence assays. Supplied by ControlBio Co. (Almería, Spain). Ref. CBi 124 K04280
E. foetida Annelid used in virulence assays. Supplied by Lombriventa (Gerona, Spain). 63
D. magna Crustacean used in virulence assays. Supplied by Daphtoxkit™ (Creasel, Belgium). 64